Visualizing SNVs to quantify allele-specific expression in single cells

We present a high efficiency fluorescence in situ hybridization method for detecting single nucleotide variants (SNVs) on individual RNA transcripts, both exonic and intronic. We used this method to quantify allelic expression at the population and single cell level, and also to distinguish maternal...

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Detalles Bibliográficos
Autores principales: Levesque, Marshall J., Ginart, Paul, Wei, Yichen, Raj, Arjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3771873/
https://www.ncbi.nlm.nih.gov/pubmed/23913259
http://dx.doi.org/10.1038/nmeth.2589
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author Levesque, Marshall J.
Ginart, Paul
Wei, Yichen
Raj, Arjun
author_facet Levesque, Marshall J.
Ginart, Paul
Wei, Yichen
Raj, Arjun
author_sort Levesque, Marshall J.
collection PubMed
description We present a high efficiency fluorescence in situ hybridization method for detecting single nucleotide variants (SNVs) on individual RNA transcripts, both exonic and intronic. We used this method to quantify allelic expression at the population and single cell level, and also to distinguish maternal from paternal chromosomes in single cells.
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spelling pubmed-37718732014-03-01 Visualizing SNVs to quantify allele-specific expression in single cells Levesque, Marshall J. Ginart, Paul Wei, Yichen Raj, Arjun Nat Methods Article We present a high efficiency fluorescence in situ hybridization method for detecting single nucleotide variants (SNVs) on individual RNA transcripts, both exonic and intronic. We used this method to quantify allelic expression at the population and single cell level, and also to distinguish maternal from paternal chromosomes in single cells. 2013-08-04 2013-09 /pmc/articles/PMC3771873/ /pubmed/23913259 http://dx.doi.org/10.1038/nmeth.2589 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Levesque, Marshall J.
Ginart, Paul
Wei, Yichen
Raj, Arjun
Visualizing SNVs to quantify allele-specific expression in single cells
title Visualizing SNVs to quantify allele-specific expression in single cells
title_full Visualizing SNVs to quantify allele-specific expression in single cells
title_fullStr Visualizing SNVs to quantify allele-specific expression in single cells
title_full_unstemmed Visualizing SNVs to quantify allele-specific expression in single cells
title_short Visualizing SNVs to quantify allele-specific expression in single cells
title_sort visualizing snvs to quantify allele-specific expression in single cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3771873/
https://www.ncbi.nlm.nih.gov/pubmed/23913259
http://dx.doi.org/10.1038/nmeth.2589
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AT rajarjun visualizingsnvstoquantifyallelespecificexpressioninsinglecells

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