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Monopolin recruits condensin to organize centromere DNA and repetitive DNA sequences

The establishment and maintenance of higher-order structure at centromeres is essential for accurate chromosome segregation. The monopolin complex is thought to cross-link multiple kinetochore complexes to prevent merotelic attachments that result in chromosome missegregation. This model is based on...

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Autores principales: Burrack, Laura S., Applen Clancey, Shelly E., Chacón, Jeremy M., Gardner, Melissa K., Berman, Judith
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3771944/
https://www.ncbi.nlm.nih.gov/pubmed/23885115
http://dx.doi.org/10.1091/mbc.E13-05-0229
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author Burrack, Laura S.
Applen Clancey, Shelly E.
Chacón, Jeremy M.
Gardner, Melissa K.
Berman, Judith
author_facet Burrack, Laura S.
Applen Clancey, Shelly E.
Chacón, Jeremy M.
Gardner, Melissa K.
Berman, Judith
author_sort Burrack, Laura S.
collection PubMed
description The establishment and maintenance of higher-order structure at centromeres is essential for accurate chromosome segregation. The monopolin complex is thought to cross-link multiple kinetochore complexes to prevent merotelic attachments that result in chromosome missegregation. This model is based on structural analysis and the requirement that monopolin execute mitotic and meiotic chromosome segregation in Schizosaccharomyces pombe, which has more than one kinetochore–microtubule attachment/centromere, and co-orient sister chromatids in meiosis I in Saccharomyces cerevisiae. Recent data from S. pombe suggest an alternative possibility: that the recruitment of condensin is the primary function of monopolin. Here we test these models using the yeast Candida albicans. C. albicans cells lacking monopolin exhibit defects in chromosome segregation, increased distance between centromeres, and decreased stability of several types of repeat DNA. Of note, changing kinetochore–microtubule copy number from one to more than one kinetochore–microtubule/centromere does not alter the requirement for monopolin. Furthermore, monopolin recruits condensin to C. albicans centromeres, and overexpression of condensin suppresses chromosome segregation defects in strains lacking monopolin. We propose that the key function of monopolin is to recruit condensin in order to promote the assembly of higher-order structure at centromere and repetitive DNA.
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spelling pubmed-37719442013-11-30 Monopolin recruits condensin to organize centromere DNA and repetitive DNA sequences Burrack, Laura S. Applen Clancey, Shelly E. Chacón, Jeremy M. Gardner, Melissa K. Berman, Judith Mol Biol Cell Articles The establishment and maintenance of higher-order structure at centromeres is essential for accurate chromosome segregation. The monopolin complex is thought to cross-link multiple kinetochore complexes to prevent merotelic attachments that result in chromosome missegregation. This model is based on structural analysis and the requirement that monopolin execute mitotic and meiotic chromosome segregation in Schizosaccharomyces pombe, which has more than one kinetochore–microtubule attachment/centromere, and co-orient sister chromatids in meiosis I in Saccharomyces cerevisiae. Recent data from S. pombe suggest an alternative possibility: that the recruitment of condensin is the primary function of monopolin. Here we test these models using the yeast Candida albicans. C. albicans cells lacking monopolin exhibit defects in chromosome segregation, increased distance between centromeres, and decreased stability of several types of repeat DNA. Of note, changing kinetochore–microtubule copy number from one to more than one kinetochore–microtubule/centromere does not alter the requirement for monopolin. Furthermore, monopolin recruits condensin to C. albicans centromeres, and overexpression of condensin suppresses chromosome segregation defects in strains lacking monopolin. We propose that the key function of monopolin is to recruit condensin in order to promote the assembly of higher-order structure at centromere and repetitive DNA. The American Society for Cell Biology 2013-09-15 /pmc/articles/PMC3771944/ /pubmed/23885115 http://dx.doi.org/10.1091/mbc.E13-05-0229 Text en © 2013 Burrack et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Burrack, Laura S.
Applen Clancey, Shelly E.
Chacón, Jeremy M.
Gardner, Melissa K.
Berman, Judith
Monopolin recruits condensin to organize centromere DNA and repetitive DNA sequences
title Monopolin recruits condensin to organize centromere DNA and repetitive DNA sequences
title_full Monopolin recruits condensin to organize centromere DNA and repetitive DNA sequences
title_fullStr Monopolin recruits condensin to organize centromere DNA and repetitive DNA sequences
title_full_unstemmed Monopolin recruits condensin to organize centromere DNA and repetitive DNA sequences
title_short Monopolin recruits condensin to organize centromere DNA and repetitive DNA sequences
title_sort monopolin recruits condensin to organize centromere dna and repetitive dna sequences
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3771944/
https://www.ncbi.nlm.nih.gov/pubmed/23885115
http://dx.doi.org/10.1091/mbc.E13-05-0229
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