Cargando…

Deletion of Lipoprotein PG0717 in Porphyromonas gingivalis W83 Reduces Gingipain Activity and Alters Trafficking in and Response by Host Cells

P. gingivalis (Pg), a causative agent of chronic generalized periodontitis, has been implicated in promoting cardiovascular disease. Expression of lipoprotein gene PG0717 of Pg strain W83 was found to be transiently upregulated during invasion of human coronary artery endothelial cells (HCAEC), sugg...

Descripción completa

Detalles Bibliográficos
Autores principales: Reyes, Leticia, Eiler-McManis, Eileen, Rodrigues, Paulo H., Chadda, Amandeep S., Wallet, Shannon M., Bélanger, Myriam, Barrett, Amanda G., Alvarez, Sophie, Akin, Debra, Dunn, William A., Progulske-Fox, Ann
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3772042/
https://www.ncbi.nlm.nih.gov/pubmed/24069284
http://dx.doi.org/10.1371/journal.pone.0074230
_version_ 1782284272492609536
author Reyes, Leticia
Eiler-McManis, Eileen
Rodrigues, Paulo H.
Chadda, Amandeep S.
Wallet, Shannon M.
Bélanger, Myriam
Barrett, Amanda G.
Alvarez, Sophie
Akin, Debra
Dunn, William A.
Progulske-Fox, Ann
author_facet Reyes, Leticia
Eiler-McManis, Eileen
Rodrigues, Paulo H.
Chadda, Amandeep S.
Wallet, Shannon M.
Bélanger, Myriam
Barrett, Amanda G.
Alvarez, Sophie
Akin, Debra
Dunn, William A.
Progulske-Fox, Ann
author_sort Reyes, Leticia
collection PubMed
description P. gingivalis (Pg), a causative agent of chronic generalized periodontitis, has been implicated in promoting cardiovascular disease. Expression of lipoprotein gene PG0717 of Pg strain W83 was found to be transiently upregulated during invasion of human coronary artery endothelial cells (HCAEC), suggesting this protein may be involved in virulence. We characterized the virulence phenotype of a PG0717 deletion mutant of pg W83. There were no differences in the ability of W83Δ717 to adhere and invade HCAEC. However, the increased proportion of internalized W83 at 24 hours post-inoculation was not observed with W83∆717. Deletion of PG0717 also impaired the ability of W83 to usurp the autophagic pathway in HCAEC and to induce autophagy in Saos-2 sarcoma cells. HCAEC infected with W83Δ717 also secreted significantly greater amounts of MCP-1, IL-8, IL-6, GM-CSF, and soluble ICAM-1, VCAM-1, and E-selectin when compared to W83. Further characterization of W83Δ717 revealed that neither capsule nor lipid A structure was affected by deletion of PG0717. Interestingly, the activity of both arginine (Rgp) and lysine (Kgp) gingipains was reduced in whole-cell extracts and culture supernatant of W83Δ717. RT-PCR revealed a corresponding decrease in transcription of rgpB but not rgpA or kgp. Quantitative proteome studies of the two strains revealed that both RgpA and RgpB, along with putative virulence factors peptidylarginine deiminase and Clp protease were significantly decreased in the W83Δ717. Our results suggest that PG0717 has pleiotropic effects on W83 that affect microbial induced manipulation of host responses important for microbial clearance and infection control.
format Online
Article
Text
id pubmed-3772042
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-37720422013-09-25 Deletion of Lipoprotein PG0717 in Porphyromonas gingivalis W83 Reduces Gingipain Activity and Alters Trafficking in and Response by Host Cells Reyes, Leticia Eiler-McManis, Eileen Rodrigues, Paulo H. Chadda, Amandeep S. Wallet, Shannon M. Bélanger, Myriam Barrett, Amanda G. Alvarez, Sophie Akin, Debra Dunn, William A. Progulske-Fox, Ann PLoS One Research Article P. gingivalis (Pg), a causative agent of chronic generalized periodontitis, has been implicated in promoting cardiovascular disease. Expression of lipoprotein gene PG0717 of Pg strain W83 was found to be transiently upregulated during invasion of human coronary artery endothelial cells (HCAEC), suggesting this protein may be involved in virulence. We characterized the virulence phenotype of a PG0717 deletion mutant of pg W83. There were no differences in the ability of W83Δ717 to adhere and invade HCAEC. However, the increased proportion of internalized W83 at 24 hours post-inoculation was not observed with W83∆717. Deletion of PG0717 also impaired the ability of W83 to usurp the autophagic pathway in HCAEC and to induce autophagy in Saos-2 sarcoma cells. HCAEC infected with W83Δ717 also secreted significantly greater amounts of MCP-1, IL-8, IL-6, GM-CSF, and soluble ICAM-1, VCAM-1, and E-selectin when compared to W83. Further characterization of W83Δ717 revealed that neither capsule nor lipid A structure was affected by deletion of PG0717. Interestingly, the activity of both arginine (Rgp) and lysine (Kgp) gingipains was reduced in whole-cell extracts and culture supernatant of W83Δ717. RT-PCR revealed a corresponding decrease in transcription of rgpB but not rgpA or kgp. Quantitative proteome studies of the two strains revealed that both RgpA and RgpB, along with putative virulence factors peptidylarginine deiminase and Clp protease were significantly decreased in the W83Δ717. Our results suggest that PG0717 has pleiotropic effects on W83 that affect microbial induced manipulation of host responses important for microbial clearance and infection control. Public Library of Science 2013-09-12 /pmc/articles/PMC3772042/ /pubmed/24069284 http://dx.doi.org/10.1371/journal.pone.0074230 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
spellingShingle Research Article
Reyes, Leticia
Eiler-McManis, Eileen
Rodrigues, Paulo H.
Chadda, Amandeep S.
Wallet, Shannon M.
Bélanger, Myriam
Barrett, Amanda G.
Alvarez, Sophie
Akin, Debra
Dunn, William A.
Progulske-Fox, Ann
Deletion of Lipoprotein PG0717 in Porphyromonas gingivalis W83 Reduces Gingipain Activity and Alters Trafficking in and Response by Host Cells
title Deletion of Lipoprotein PG0717 in Porphyromonas gingivalis W83 Reduces Gingipain Activity and Alters Trafficking in and Response by Host Cells
title_full Deletion of Lipoprotein PG0717 in Porphyromonas gingivalis W83 Reduces Gingipain Activity and Alters Trafficking in and Response by Host Cells
title_fullStr Deletion of Lipoprotein PG0717 in Porphyromonas gingivalis W83 Reduces Gingipain Activity and Alters Trafficking in and Response by Host Cells
title_full_unstemmed Deletion of Lipoprotein PG0717 in Porphyromonas gingivalis W83 Reduces Gingipain Activity and Alters Trafficking in and Response by Host Cells
title_short Deletion of Lipoprotein PG0717 in Porphyromonas gingivalis W83 Reduces Gingipain Activity and Alters Trafficking in and Response by Host Cells
title_sort deletion of lipoprotein pg0717 in porphyromonas gingivalis w83 reduces gingipain activity and alters trafficking in and response by host cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3772042/
https://www.ncbi.nlm.nih.gov/pubmed/24069284
http://dx.doi.org/10.1371/journal.pone.0074230
work_keys_str_mv AT reyesleticia deletionoflipoproteinpg0717inporphyromonasgingivalisw83reducesgingipainactivityandalterstraffickinginandresponsebyhostcells
AT eilermcmaniseileen deletionoflipoproteinpg0717inporphyromonasgingivalisw83reducesgingipainactivityandalterstraffickinginandresponsebyhostcells
AT rodriguespauloh deletionoflipoproteinpg0717inporphyromonasgingivalisw83reducesgingipainactivityandalterstraffickinginandresponsebyhostcells
AT chaddaamandeeps deletionoflipoproteinpg0717inporphyromonasgingivalisw83reducesgingipainactivityandalterstraffickinginandresponsebyhostcells
AT walletshannonm deletionoflipoproteinpg0717inporphyromonasgingivalisw83reducesgingipainactivityandalterstraffickinginandresponsebyhostcells
AT belangermyriam deletionoflipoproteinpg0717inporphyromonasgingivalisw83reducesgingipainactivityandalterstraffickinginandresponsebyhostcells
AT barrettamandag deletionoflipoproteinpg0717inporphyromonasgingivalisw83reducesgingipainactivityandalterstraffickinginandresponsebyhostcells
AT alvarezsophie deletionoflipoproteinpg0717inporphyromonasgingivalisw83reducesgingipainactivityandalterstraffickinginandresponsebyhostcells
AT akindebra deletionoflipoproteinpg0717inporphyromonasgingivalisw83reducesgingipainactivityandalterstraffickinginandresponsebyhostcells
AT dunnwilliama deletionoflipoproteinpg0717inporphyromonasgingivalisw83reducesgingipainactivityandalterstraffickinginandresponsebyhostcells
AT progulskefoxann deletionoflipoproteinpg0717inporphyromonasgingivalisw83reducesgingipainactivityandalterstraffickinginandresponsebyhostcells