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Direct Cloning of Isogenic Murine DNA in Yeast and Relevance of Isogenicity for Targeting in Embryonic Stem Cells

Efficient gene targeting in embryonic stem cells requires that modifying DNA sequences are identical to those in the targeted chromosomal locus. Yet, there is a paucity of isogenic genomic clones for human cell lines and PCR amplification cannot be used in many mutation-sensitive applications. Here,...

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Detalles Bibliográficos
Autores principales:	Andréasson, Claes, Schick, Anna J., Pfeiffer, Susanne M., Sarov, Mihail, Stewart, Francis, Wurst, Wolfgang, Schick, Joel A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2013
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3772885/ https://www.ncbi.nlm.nih.gov/pubmed/24058528 http://dx.doi.org/10.1371/journal.pone.0074207

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