Cargando…
Direct Cloning of Isogenic Murine DNA in Yeast and Relevance of Isogenicity for Targeting in Embryonic Stem Cells
Efficient gene targeting in embryonic stem cells requires that modifying DNA sequences are identical to those in the targeted chromosomal locus. Yet, there is a paucity of isogenic genomic clones for human cell lines and PCR amplification cannot be used in many mutation-sensitive applications. Here,...
Autores principales: | Andréasson, Claes, Schick, Anna J., Pfeiffer, Susanne M., Sarov, Mihail, Stewart, Francis, Wurst, Wolfgang, Schick, Joel A. |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3772885/ https://www.ncbi.nlm.nih.gov/pubmed/24058528 http://dx.doi.org/10.1371/journal.pone.0074207 |
Ejemplares similares
-
Targeted isolation of cloned genomic regions by recombineering for haplotype phasing and isogenic targeting
por: Nedelkova, Marta, et al.
Publicado: (2011) -
Genetic screens in isogenic mammalian cell lines without single cell cloning
por: DeWeirdt, Peter C., et al.
Publicado: (2020) -
Metabolic heterogeneity and cross-feeding within isogenic yeast populations captured by DILAC
por: Kamrad, Stephan, et al.
Publicado: (2023) -
Fitness of Isogenic Colony Morphology Variants of Pseudomonas aeruginosa in Murine Airway Infection
por: Rakhimova, Elza, et al.
Publicado: (2008) -
Comparison of the molecular profiles of human embryonic and induced pluripotent stem cells of isogenic origin
por: Mallon, Barbara S., et al.
Publicado: (2013)