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Effect of Gambisan on the Inhibition of Adipogenesis in 3T3-L1 Adipocytes
This study was conducted to explore the antiadipogenic effect and possible mechanism of Gambisan on 3T3-L1 cells. For quality control, Gambisan was standardized by HPLC and the standard compounds ephedrine, epigallocatechin-3-gallate, and caffeine were screened. Cultured 3T3-L1 cells that had been i...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3773429/ https://www.ncbi.nlm.nih.gov/pubmed/24069055 http://dx.doi.org/10.1155/2013/789067 |
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author | Kang, Jung Won Nam, Dongwoo Kim, Kun Hyung Huh, Jeong-Eun Lee, Jae-Dong |
author_facet | Kang, Jung Won Nam, Dongwoo Kim, Kun Hyung Huh, Jeong-Eun Lee, Jae-Dong |
author_sort | Kang, Jung Won |
collection | PubMed |
description | This study was conducted to explore the antiadipogenic effect and possible mechanism of Gambisan on 3T3-L1 cells. For quality control, Gambisan was standardized by HPLC and the standard compounds ephedrine, epigallocatechin-3-gallate, and caffeine were screened. Cultured 3T3-L1 cells that had been induced to differentiate were treated with various concentrations of Gambisan or its major component extracts (Ephedra intermedia Schrenk, Atractylodes lancea DC., and Thea sinensis L.) for 72 hours for MTT assay to determine cell viability or 10 days for LDH assay, triglyceride assay, DNA content measurement, Oil red O staining, RT-PCR, and western blot. Gambisan significantly inhibited adipogenesis in 3T3-L1 cells by reducing triglyceride contents and lipid accumulation in a dose-dependent manner without obvious cytotoxicity. Viability and DNA content in 3T3-L1 cells treated with Gambisan were significantly higher than cells treated with the major component extracts at every concentration. The anti-adipogenic effects of Gambisan appeared to be mediated by a significant downregulation of the expression of lipoprotein lipase mRNA and PPARγ, C/EBPα, and SREBP-1 protein apart from the expression of hormone-sensitive lipase. Gambisan could act as a possible therapeutic agent for obesity. However, further studies including in vivo assays and clinical trials are needed to confirm the efficacy, safety and mechanisms of the antiobesity effects of Gambisan. |
format | Online Article Text |
id | pubmed-3773429 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-37734292013-09-25 Effect of Gambisan on the Inhibition of Adipogenesis in 3T3-L1 Adipocytes Kang, Jung Won Nam, Dongwoo Kim, Kun Hyung Huh, Jeong-Eun Lee, Jae-Dong Evid Based Complement Alternat Med Research Article This study was conducted to explore the antiadipogenic effect and possible mechanism of Gambisan on 3T3-L1 cells. For quality control, Gambisan was standardized by HPLC and the standard compounds ephedrine, epigallocatechin-3-gallate, and caffeine were screened. Cultured 3T3-L1 cells that had been induced to differentiate were treated with various concentrations of Gambisan or its major component extracts (Ephedra intermedia Schrenk, Atractylodes lancea DC., and Thea sinensis L.) for 72 hours for MTT assay to determine cell viability or 10 days for LDH assay, triglyceride assay, DNA content measurement, Oil red O staining, RT-PCR, and western blot. Gambisan significantly inhibited adipogenesis in 3T3-L1 cells by reducing triglyceride contents and lipid accumulation in a dose-dependent manner without obvious cytotoxicity. Viability and DNA content in 3T3-L1 cells treated with Gambisan were significantly higher than cells treated with the major component extracts at every concentration. The anti-adipogenic effects of Gambisan appeared to be mediated by a significant downregulation of the expression of lipoprotein lipase mRNA and PPARγ, C/EBPα, and SREBP-1 protein apart from the expression of hormone-sensitive lipase. Gambisan could act as a possible therapeutic agent for obesity. However, further studies including in vivo assays and clinical trials are needed to confirm the efficacy, safety and mechanisms of the antiobesity effects of Gambisan. Hindawi Publishing Corporation 2013 2013-08-20 /pmc/articles/PMC3773429/ /pubmed/24069055 http://dx.doi.org/10.1155/2013/789067 Text en Copyright © 2013 Jung Won Kang et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Kang, Jung Won Nam, Dongwoo Kim, Kun Hyung Huh, Jeong-Eun Lee, Jae-Dong Effect of Gambisan on the Inhibition of Adipogenesis in 3T3-L1 Adipocytes |
title | Effect of Gambisan on the Inhibition of Adipogenesis in 3T3-L1 Adipocytes |
title_full | Effect of Gambisan on the Inhibition of Adipogenesis in 3T3-L1 Adipocytes |
title_fullStr | Effect of Gambisan on the Inhibition of Adipogenesis in 3T3-L1 Adipocytes |
title_full_unstemmed | Effect of Gambisan on the Inhibition of Adipogenesis in 3T3-L1 Adipocytes |
title_short | Effect of Gambisan on the Inhibition of Adipogenesis in 3T3-L1 Adipocytes |
title_sort | effect of gambisan on the inhibition of adipogenesis in 3t3-l1 adipocytes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3773429/ https://www.ncbi.nlm.nih.gov/pubmed/24069055 http://dx.doi.org/10.1155/2013/789067 |
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