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Effect of Gambisan on the Inhibition of Adipogenesis in 3T3-L1 Adipocytes

This study was conducted to explore the antiadipogenic effect and possible mechanism of Gambisan on 3T3-L1 cells. For quality control, Gambisan was standardized by HPLC and the standard compounds ephedrine, epigallocatechin-3-gallate, and caffeine were screened. Cultured 3T3-L1 cells that had been i...

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Autores principales: Kang, Jung Won, Nam, Dongwoo, Kim, Kun Hyung, Huh, Jeong-Eun, Lee, Jae-Dong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3773429/
https://www.ncbi.nlm.nih.gov/pubmed/24069055
http://dx.doi.org/10.1155/2013/789067
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author Kang, Jung Won
Nam, Dongwoo
Kim, Kun Hyung
Huh, Jeong-Eun
Lee, Jae-Dong
author_facet Kang, Jung Won
Nam, Dongwoo
Kim, Kun Hyung
Huh, Jeong-Eun
Lee, Jae-Dong
author_sort Kang, Jung Won
collection PubMed
description This study was conducted to explore the antiadipogenic effect and possible mechanism of Gambisan on 3T3-L1 cells. For quality control, Gambisan was standardized by HPLC and the standard compounds ephedrine, epigallocatechin-3-gallate, and caffeine were screened. Cultured 3T3-L1 cells that had been induced to differentiate were treated with various concentrations of Gambisan or its major component extracts (Ephedra intermedia Schrenk, Atractylodes lancea DC., and Thea sinensis L.) for 72 hours for MTT assay to determine cell viability or 10 days for LDH assay, triglyceride assay, DNA content measurement, Oil red O staining, RT-PCR, and western blot. Gambisan significantly inhibited adipogenesis in 3T3-L1 cells by reducing triglyceride contents and lipid accumulation in a dose-dependent manner without obvious cytotoxicity. Viability and DNA content in 3T3-L1 cells treated with Gambisan were significantly higher than cells treated with the major component extracts at every concentration. The anti-adipogenic effects of Gambisan appeared to be mediated by a significant downregulation of the expression of lipoprotein lipase mRNA and PPARγ, C/EBPα, and SREBP-1 protein apart from the expression of hormone-sensitive lipase. Gambisan could act as a possible therapeutic agent for obesity. However, further studies including in vivo assays and clinical trials are needed to confirm the efficacy, safety and mechanisms of the antiobesity effects of Gambisan.
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spelling pubmed-37734292013-09-25 Effect of Gambisan on the Inhibition of Adipogenesis in 3T3-L1 Adipocytes Kang, Jung Won Nam, Dongwoo Kim, Kun Hyung Huh, Jeong-Eun Lee, Jae-Dong Evid Based Complement Alternat Med Research Article This study was conducted to explore the antiadipogenic effect and possible mechanism of Gambisan on 3T3-L1 cells. For quality control, Gambisan was standardized by HPLC and the standard compounds ephedrine, epigallocatechin-3-gallate, and caffeine were screened. Cultured 3T3-L1 cells that had been induced to differentiate were treated with various concentrations of Gambisan or its major component extracts (Ephedra intermedia Schrenk, Atractylodes lancea DC., and Thea sinensis L.) for 72 hours for MTT assay to determine cell viability or 10 days for LDH assay, triglyceride assay, DNA content measurement, Oil red O staining, RT-PCR, and western blot. Gambisan significantly inhibited adipogenesis in 3T3-L1 cells by reducing triglyceride contents and lipid accumulation in a dose-dependent manner without obvious cytotoxicity. Viability and DNA content in 3T3-L1 cells treated with Gambisan were significantly higher than cells treated with the major component extracts at every concentration. The anti-adipogenic effects of Gambisan appeared to be mediated by a significant downregulation of the expression of lipoprotein lipase mRNA and PPARγ, C/EBPα, and SREBP-1 protein apart from the expression of hormone-sensitive lipase. Gambisan could act as a possible therapeutic agent for obesity. However, further studies including in vivo assays and clinical trials are needed to confirm the efficacy, safety and mechanisms of the antiobesity effects of Gambisan. Hindawi Publishing Corporation 2013 2013-08-20 /pmc/articles/PMC3773429/ /pubmed/24069055 http://dx.doi.org/10.1155/2013/789067 Text en Copyright © 2013 Jung Won Kang et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kang, Jung Won
Nam, Dongwoo
Kim, Kun Hyung
Huh, Jeong-Eun
Lee, Jae-Dong
Effect of Gambisan on the Inhibition of Adipogenesis in 3T3-L1 Adipocytes
title Effect of Gambisan on the Inhibition of Adipogenesis in 3T3-L1 Adipocytes
title_full Effect of Gambisan on the Inhibition of Adipogenesis in 3T3-L1 Adipocytes
title_fullStr Effect of Gambisan on the Inhibition of Adipogenesis in 3T3-L1 Adipocytes
title_full_unstemmed Effect of Gambisan on the Inhibition of Adipogenesis in 3T3-L1 Adipocytes
title_short Effect of Gambisan on the Inhibition of Adipogenesis in 3T3-L1 Adipocytes
title_sort effect of gambisan on the inhibition of adipogenesis in 3t3-l1 adipocytes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3773429/
https://www.ncbi.nlm.nih.gov/pubmed/24069055
http://dx.doi.org/10.1155/2013/789067
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