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Mesenchymal Stem Cells and Platelet Gel Improve Bone Deposition within CAD-CAM Custom-Made Ceramic HA Scaffolds for Condyle Substitution

Purpose. This study evaluated the efficacy of a regenerative approach using mesenchymal stem cells (MSCs) and CAD-CAM customized pure and porous hydroxyapatite (HA) scaffolds to replace the temporomandibular joint (TMJ) condyle. Methods. Pure HA scaffolds with a 70% total porosity volume were protot...

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Autores principales: Ciocca, L., Donati, D., Ragazzini, S., Dozza, B., Rossi, F., Fantini, M., Spadari, A., Romagnoli, N., Landi, E., Tampieri, A., Piattelli, A., Iezzi, G., Scotti, R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3773948/
https://www.ncbi.nlm.nih.gov/pubmed/24073409
http://dx.doi.org/10.1155/2013/549762
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author Ciocca, L.
Donati, D.
Ragazzini, S.
Dozza, B.
Rossi, F.
Fantini, M.
Spadari, A.
Romagnoli, N.
Landi, E.
Tampieri, A.
Piattelli, A.
Iezzi, G.
Scotti, R.
author_facet Ciocca, L.
Donati, D.
Ragazzini, S.
Dozza, B.
Rossi, F.
Fantini, M.
Spadari, A.
Romagnoli, N.
Landi, E.
Tampieri, A.
Piattelli, A.
Iezzi, G.
Scotti, R.
author_sort Ciocca, L.
collection PubMed
description Purpose. This study evaluated the efficacy of a regenerative approach using mesenchymal stem cells (MSCs) and CAD-CAM customized pure and porous hydroxyapatite (HA) scaffolds to replace the temporomandibular joint (TMJ) condyle. Methods. Pure HA scaffolds with a 70% total porosity volume were prototyped using CAD-CAM technology to replace the two temporomandibular condyles (left and right) of the same animal. MSCs were derived from the aspirated iliac crest bone marrow, and platelets were obtained from the venous blood of the sheep. Custom-made surgical guides were created by direct metal laser sintering and were used to export the virtual planning of the bone cut lines into the surgical environment. Sheep were sacrificed 4 months postoperatively. The HA scaffolds were explanted, histological specimens were prepared, and histomorphometric analysis was performed. Results. Analysis of the porosity reduction for apposition of newly formed bone showed a statistically significant difference in bone formation between condyles loaded with MSC and condyles without (P < 0.05). The bone ingrowth (BI) relative values of split-mouth comparison (right versus left side) showed a significant difference between condyles with and without MSCs (P < 0.05). Analysis of the test and control sides in the same animal using a split-mouth study design was performed; the condyle with MSCs showed greater bone formation. Conclusion. The split-mouth design confirmed an increment of bone regeneration into the HA scaffold of up to 797% upon application of MSCs.
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spelling pubmed-37739482013-09-26 Mesenchymal Stem Cells and Platelet Gel Improve Bone Deposition within CAD-CAM Custom-Made Ceramic HA Scaffolds for Condyle Substitution Ciocca, L. Donati, D. Ragazzini, S. Dozza, B. Rossi, F. Fantini, M. Spadari, A. Romagnoli, N. Landi, E. Tampieri, A. Piattelli, A. Iezzi, G. Scotti, R. Biomed Res Int Research Article Purpose. This study evaluated the efficacy of a regenerative approach using mesenchymal stem cells (MSCs) and CAD-CAM customized pure and porous hydroxyapatite (HA) scaffolds to replace the temporomandibular joint (TMJ) condyle. Methods. Pure HA scaffolds with a 70% total porosity volume were prototyped using CAD-CAM technology to replace the two temporomandibular condyles (left and right) of the same animal. MSCs were derived from the aspirated iliac crest bone marrow, and platelets were obtained from the venous blood of the sheep. Custom-made surgical guides were created by direct metal laser sintering and were used to export the virtual planning of the bone cut lines into the surgical environment. Sheep were sacrificed 4 months postoperatively. The HA scaffolds were explanted, histological specimens were prepared, and histomorphometric analysis was performed. Results. Analysis of the porosity reduction for apposition of newly formed bone showed a statistically significant difference in bone formation between condyles loaded with MSC and condyles without (P < 0.05). The bone ingrowth (BI) relative values of split-mouth comparison (right versus left side) showed a significant difference between condyles with and without MSCs (P < 0.05). Analysis of the test and control sides in the same animal using a split-mouth study design was performed; the condyle with MSCs showed greater bone formation. Conclusion. The split-mouth design confirmed an increment of bone regeneration into the HA scaffold of up to 797% upon application of MSCs. Hindawi Publishing Corporation 2013 2013-09-01 /pmc/articles/PMC3773948/ /pubmed/24073409 http://dx.doi.org/10.1155/2013/549762 Text en Copyright © 2013 L. Ciocca et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ciocca, L.
Donati, D.
Ragazzini, S.
Dozza, B.
Rossi, F.
Fantini, M.
Spadari, A.
Romagnoli, N.
Landi, E.
Tampieri, A.
Piattelli, A.
Iezzi, G.
Scotti, R.
Mesenchymal Stem Cells and Platelet Gel Improve Bone Deposition within CAD-CAM Custom-Made Ceramic HA Scaffolds for Condyle Substitution
title Mesenchymal Stem Cells and Platelet Gel Improve Bone Deposition within CAD-CAM Custom-Made Ceramic HA Scaffolds for Condyle Substitution
title_full Mesenchymal Stem Cells and Platelet Gel Improve Bone Deposition within CAD-CAM Custom-Made Ceramic HA Scaffolds for Condyle Substitution
title_fullStr Mesenchymal Stem Cells and Platelet Gel Improve Bone Deposition within CAD-CAM Custom-Made Ceramic HA Scaffolds for Condyle Substitution
title_full_unstemmed Mesenchymal Stem Cells and Platelet Gel Improve Bone Deposition within CAD-CAM Custom-Made Ceramic HA Scaffolds for Condyle Substitution
title_short Mesenchymal Stem Cells and Platelet Gel Improve Bone Deposition within CAD-CAM Custom-Made Ceramic HA Scaffolds for Condyle Substitution
title_sort mesenchymal stem cells and platelet gel improve bone deposition within cad-cam custom-made ceramic ha scaffolds for condyle substitution
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3773948/
https://www.ncbi.nlm.nih.gov/pubmed/24073409
http://dx.doi.org/10.1155/2013/549762
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