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Effects of Constitutive β-Catenin Activation on Vertebral Bone Growth and Remodeling at Different Postnatal Stages in Mice
BACKGROUND AND OBJECTIVE: The Wnt/β-catenin signaling pathway is essential for controlling bone mass; however, little is known about the variable effects of the constitutive activation of β-catenin (CA-β-catenin) on bone growth and remodeling at different postnatal stages. The goal of the present st...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3774640/ https://www.ncbi.nlm.nih.gov/pubmed/24066100 http://dx.doi.org/10.1371/journal.pone.0074093 |
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author | Jia, Min Chen, Sixu Zhang, Bo Liang, Huaping Feng, Jianquan Zong, Zhaowen |
author_facet | Jia, Min Chen, Sixu Zhang, Bo Liang, Huaping Feng, Jianquan Zong, Zhaowen |
author_sort | Jia, Min |
collection | PubMed |
description | BACKGROUND AND OBJECTIVE: The Wnt/β-catenin signaling pathway is essential for controlling bone mass; however, little is known about the variable effects of the constitutive activation of β-catenin (CA-β-catenin) on bone growth and remodeling at different postnatal stages. The goal of the present study was to observe the effects of CA-β-catenin on vertebral bone growth and remodeling in mice at different postnatal stages. In particular, special attention was paid to whether CA-β-catenin has detrimental effects on these processes. METHODS: Catnblox(ex 3) mice were crossed with mice expressing the TM-inducible Cre fusion protein, which could be activated at designated time points via injection of tamoxifen. β-catenin was stabilized by tamoxifen injection 3 days, and 2, 4, 5, and 7 months after birth, and the effects lasted for one month. Radiographic imaging, micro-computed tomography, immunohistochemistry, and safranin O and tartrate-resistant acid phosphatase staining were employed to observe the effects of CA-β-catenin on vertebral bone growth and remodeling. RESULTS: CA-β-catenin in both early (3 days after birth) and late stages (2, 4, 5, and 7 months after birth) increased bone formation and decreased bone resorption, which together increased vertebral bone volume. However, when β-catenin was stabilized in the early stage, vertebral linear growth was retarded, and the mice demonstrated shorter statures. In addition, the newly formed bone was mainly immature and located close to the growth plate. In contrast, when β-catenin was stabilized in the late stage, vertebral linear growth was unaffected, and the newly formed bone was mainly mature and evenly distributed throughout the vertebral body. CONCLUSIONS: CA-β-catenin in both early and late stages of growth can increase vertebral bone volume, but β-catenin has differential effects on vertebral growth and remodeling when activated at different postnatal stages. |
format | Online Article Text |
id | pubmed-3774640 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-37746402013-09-24 Effects of Constitutive β-Catenin Activation on Vertebral Bone Growth and Remodeling at Different Postnatal Stages in Mice Jia, Min Chen, Sixu Zhang, Bo Liang, Huaping Feng, Jianquan Zong, Zhaowen PLoS One Research Article BACKGROUND AND OBJECTIVE: The Wnt/β-catenin signaling pathway is essential for controlling bone mass; however, little is known about the variable effects of the constitutive activation of β-catenin (CA-β-catenin) on bone growth and remodeling at different postnatal stages. The goal of the present study was to observe the effects of CA-β-catenin on vertebral bone growth and remodeling in mice at different postnatal stages. In particular, special attention was paid to whether CA-β-catenin has detrimental effects on these processes. METHODS: Catnblox(ex 3) mice were crossed with mice expressing the TM-inducible Cre fusion protein, which could be activated at designated time points via injection of tamoxifen. β-catenin was stabilized by tamoxifen injection 3 days, and 2, 4, 5, and 7 months after birth, and the effects lasted for one month. Radiographic imaging, micro-computed tomography, immunohistochemistry, and safranin O and tartrate-resistant acid phosphatase staining were employed to observe the effects of CA-β-catenin on vertebral bone growth and remodeling. RESULTS: CA-β-catenin in both early (3 days after birth) and late stages (2, 4, 5, and 7 months after birth) increased bone formation and decreased bone resorption, which together increased vertebral bone volume. However, when β-catenin was stabilized in the early stage, vertebral linear growth was retarded, and the mice demonstrated shorter statures. In addition, the newly formed bone was mainly immature and located close to the growth plate. In contrast, when β-catenin was stabilized in the late stage, vertebral linear growth was unaffected, and the newly formed bone was mainly mature and evenly distributed throughout the vertebral body. CONCLUSIONS: CA-β-catenin in both early and late stages of growth can increase vertebral bone volume, but β-catenin has differential effects on vertebral growth and remodeling when activated at different postnatal stages. Public Library of Science 2013-09-16 /pmc/articles/PMC3774640/ /pubmed/24066100 http://dx.doi.org/10.1371/journal.pone.0074093 Text en © 2013 Jia et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Jia, Min Chen, Sixu Zhang, Bo Liang, Huaping Feng, Jianquan Zong, Zhaowen Effects of Constitutive β-Catenin Activation on Vertebral Bone Growth and Remodeling at Different Postnatal Stages in Mice |
title | Effects of Constitutive β-Catenin Activation on Vertebral Bone Growth and Remodeling at Different Postnatal Stages in Mice |
title_full | Effects of Constitutive β-Catenin Activation on Vertebral Bone Growth and Remodeling at Different Postnatal Stages in Mice |
title_fullStr | Effects of Constitutive β-Catenin Activation on Vertebral Bone Growth and Remodeling at Different Postnatal Stages in Mice |
title_full_unstemmed | Effects of Constitutive β-Catenin Activation on Vertebral Bone Growth and Remodeling at Different Postnatal Stages in Mice |
title_short | Effects of Constitutive β-Catenin Activation on Vertebral Bone Growth and Remodeling at Different Postnatal Stages in Mice |
title_sort | effects of constitutive β-catenin activation on vertebral bone growth and remodeling at different postnatal stages in mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3774640/ https://www.ncbi.nlm.nih.gov/pubmed/24066100 http://dx.doi.org/10.1371/journal.pone.0074093 |
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