Knockdown of ZBED6 is not associated with changes in murine preadipocyte proliferation or differentiation

ZBED6 was identified as a transcription factor that affects muscle mass and fat deposition in pigs. Mechanisms mediating effects on fat mass are unclear. The objective was to determine the effect of ZBED6 mRNA knockdown on 3T3-L1 preadipocyte differentiation and gene expression. Differentiation was...

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Detalles Bibliográficos
Autores principales: Zhang, Wei, Cline, Mark A, Liu, Dongmin, Gilbert, Elizabeth R
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Landes Bioscience 2013
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3774701/
https://www.ncbi.nlm.nih.gov/pubmed/24052901
http://dx.doi.org/10.4161/adip.26028
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author Zhang, Wei
Cline, Mark A
Liu, Dongmin
Gilbert, Elizabeth R
author_facet Zhang, Wei
Cline, Mark A
Liu, Dongmin
Gilbert, Elizabeth R
author_sort Zhang, Wei
collection PubMed
description ZBED6 was identified as a transcription factor that affects muscle mass and fat deposition in pigs. Mechanisms mediating effects on fat mass are unclear. The objective was to determine the effect of ZBED6 mRNA knockdown on 3T3-L1 preadipocyte differentiation and gene expression. Differentiation was associated with increased mRNA abundance of CEBP/α (P < 0.05), CEBP/β (P < 0.05), CEBP/δ (P < 0.05), FASN (P < 0.05), PPARγ (P < 0.05), and SREBP-1 (P < 0.05), and decreased abundance of PREF-1 (P < 0.05). Knockdown of ZBED6 was not associated with changes in mRNA abundance of selected genes, lipid accumulation, lipid droplet size, or cell number. These results suggest that ZBED6 does not play a major role in preadipocyte differentiation.
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spelling pubmed-37747012013-09-19 Knockdown of ZBED6 is not associated with changes in murine preadipocyte proliferation or differentiation Zhang, Wei Cline, Mark A Liu, Dongmin Gilbert, Elizabeth R Adipocyte Brief Report ZBED6 was identified as a transcription factor that affects muscle mass and fat deposition in pigs. Mechanisms mediating effects on fat mass are unclear. The objective was to determine the effect of ZBED6 mRNA knockdown on 3T3-L1 preadipocyte differentiation and gene expression. Differentiation was associated with increased mRNA abundance of CEBP/α (P < 0.05), CEBP/β (P < 0.05), CEBP/δ (P < 0.05), FASN (P < 0.05), PPARγ (P < 0.05), and SREBP-1 (P < 0.05), and decreased abundance of PREF-1 (P < 0.05). Knockdown of ZBED6 was not associated with changes in mRNA abundance of selected genes, lipid accumulation, lipid droplet size, or cell number. These results suggest that ZBED6 does not play a major role in preadipocyte differentiation. Landes Bioscience 2013-10-01 2013-08-13 /pmc/articles/PMC3774701/ /pubmed/24052901 http://dx.doi.org/10.4161/adip.26028 Text en Copyright © 2013 Landes Bioscience http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Brief Report
Zhang, Wei
Cline, Mark A
Liu, Dongmin
Gilbert, Elizabeth R
Knockdown of ZBED6 is not associated with changes in murine preadipocyte proliferation or differentiation
title Knockdown of ZBED6 is not associated with changes in murine preadipocyte proliferation or differentiation
title_full Knockdown of ZBED6 is not associated with changes in murine preadipocyte proliferation or differentiation
title_fullStr Knockdown of ZBED6 is not associated with changes in murine preadipocyte proliferation or differentiation
title_full_unstemmed Knockdown of ZBED6 is not associated with changes in murine preadipocyte proliferation or differentiation
title_short Knockdown of ZBED6 is not associated with changes in murine preadipocyte proliferation or differentiation
title_sort knockdown of zbed6 is not associated with changes in murine preadipocyte proliferation or differentiation
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3774701/
https://www.ncbi.nlm.nih.gov/pubmed/24052901
http://dx.doi.org/10.4161/adip.26028
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