Contrasting Quiescent G(0) Phase with Mitotic Cell Cycling in the Mouse Immune System

A transgenic mouse line expressing Fucci (fluorescent ubiquitination-based cell-cycle indicator) probes allows us to monitor the cell cycle in the hematopoietic system. Two populations with high and low intensities of Fucci signals for Cdt1(30/120) accumulation were identified by FACS analysis, and...

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Detalles Bibliográficos
Autores principales: Tomura, Michio, Sakaue-Sawano, Asako, Mori, Yoshiko, Takase-Utsugi, Mitsuyo, Hata, Akihiro, Ohtawa, Kenji, Kanagawa, Osami, Miyawaki, Atsushi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3774768/
https://www.ncbi.nlm.nih.gov/pubmed/24066072
http://dx.doi.org/10.1371/journal.pone.0073801
Descripción
Sumario:A transgenic mouse line expressing Fucci (fluorescent ubiquitination-based cell-cycle indicator) probes allows us to monitor the cell cycle in the hematopoietic system. Two populations with high and low intensities of Fucci signals for Cdt1(30/120) accumulation were identified by FACS analysis, and these correspond to quiescent G(0) and cycling G(1) cells, respectively. We observed the transition of immune cells between quiescent and proliferative phases in lymphoid organs during differentiation and immune responses.

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