Contrasting Quiescent G(0) Phase with Mitotic Cell Cycling in the Mouse Immune System

A transgenic mouse line expressing Fucci (fluorescent ubiquitination-based cell-cycle indicator) probes allows us to monitor the cell cycle in the hematopoietic system. Two populations with high and low intensities of Fucci signals for Cdt1(30/120) accumulation were identified by FACS analysis, and...

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Autores principales: Tomura, Michio, Sakaue-Sawano, Asako, Mori, Yoshiko, Takase-Utsugi, Mitsuyo, Hata, Akihiro, Ohtawa, Kenji, Kanagawa, Osami, Miyawaki, Atsushi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3774768/
https://www.ncbi.nlm.nih.gov/pubmed/24066072
http://dx.doi.org/10.1371/journal.pone.0073801
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author Tomura, Michio
Sakaue-Sawano, Asako
Mori, Yoshiko
Takase-Utsugi, Mitsuyo
Hata, Akihiro
Ohtawa, Kenji
Kanagawa, Osami
Miyawaki, Atsushi
author_facet Tomura, Michio
Sakaue-Sawano, Asako
Mori, Yoshiko
Takase-Utsugi, Mitsuyo
Hata, Akihiro
Ohtawa, Kenji
Kanagawa, Osami
Miyawaki, Atsushi
author_sort Tomura, Michio
collection PubMed
description A transgenic mouse line expressing Fucci (fluorescent ubiquitination-based cell-cycle indicator) probes allows us to monitor the cell cycle in the hematopoietic system. Two populations with high and low intensities of Fucci signals for Cdt1(30/120) accumulation were identified by FACS analysis, and these correspond to quiescent G(0) and cycling G(1) cells, respectively. We observed the transition of immune cells between quiescent and proliferative phases in lymphoid organs during differentiation and immune responses.
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spelling pubmed-37747682013-09-24 Contrasting Quiescent G(0) Phase with Mitotic Cell Cycling in the Mouse Immune System Tomura, Michio Sakaue-Sawano, Asako Mori, Yoshiko Takase-Utsugi, Mitsuyo Hata, Akihiro Ohtawa, Kenji Kanagawa, Osami Miyawaki, Atsushi PLoS One Research Article A transgenic mouse line expressing Fucci (fluorescent ubiquitination-based cell-cycle indicator) probes allows us to monitor the cell cycle in the hematopoietic system. Two populations with high and low intensities of Fucci signals for Cdt1(30/120) accumulation were identified by FACS analysis, and these correspond to quiescent G(0) and cycling G(1) cells, respectively. We observed the transition of immune cells between quiescent and proliferative phases in lymphoid organs during differentiation and immune responses. Public Library of Science 2013-09-16 /pmc/articles/PMC3774768/ /pubmed/24066072 http://dx.doi.org/10.1371/journal.pone.0073801 Text en © 2013 Tomura et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Tomura, Michio
Sakaue-Sawano, Asako
Mori, Yoshiko
Takase-Utsugi, Mitsuyo
Hata, Akihiro
Ohtawa, Kenji
Kanagawa, Osami
Miyawaki, Atsushi
Contrasting Quiescent G(0) Phase with Mitotic Cell Cycling in the Mouse Immune System
title Contrasting Quiescent G(0) Phase with Mitotic Cell Cycling in the Mouse Immune System
title_full Contrasting Quiescent G(0) Phase with Mitotic Cell Cycling in the Mouse Immune System
title_fullStr Contrasting Quiescent G(0) Phase with Mitotic Cell Cycling in the Mouse Immune System
title_full_unstemmed Contrasting Quiescent G(0) Phase with Mitotic Cell Cycling in the Mouse Immune System
title_short Contrasting Quiescent G(0) Phase with Mitotic Cell Cycling in the Mouse Immune System
title_sort contrasting quiescent g(0) phase with mitotic cell cycling in the mouse immune system
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3774768/
https://www.ncbi.nlm.nih.gov/pubmed/24066072
http://dx.doi.org/10.1371/journal.pone.0073801
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