Cargando…
The effects of acute doxorubicin treatment on proteome lysine acetylation status and apical caspases in skeletal muscle of fasted animals
BACKGROUND: Doxorubicin treatment is known to cause muscular weakness. However, the cellular mechanisms have not been elucidated. We aimed to determine the effects of acute doxorubicin treatment on proteome lysine acetylation status, an indication of the apoptotic and inflammatory environment, and t...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3774913/ https://www.ncbi.nlm.nih.gov/pubmed/23529675 http://dx.doi.org/10.1007/s13539-013-0104-z |
_version_ | 1782284539204206592 |
---|---|
author | Dirks-Naylor, Amie J. Tran, Ngan T. K. Yang, Sendra Mabolo, Raean Kouzi, Samir A. |
author_facet | Dirks-Naylor, Amie J. Tran, Ngan T. K. Yang, Sendra Mabolo, Raean Kouzi, Samir A. |
author_sort | Dirks-Naylor, Amie J. |
collection | PubMed |
description | BACKGROUND: Doxorubicin treatment is known to cause muscular weakness. However, the cellular mechanisms have not been elucidated. We aimed to determine the effects of acute doxorubicin treatment on proteome lysine acetylation status, an indication of the apoptotic and inflammatory environment, and the expression and activation of various apical caspases involved in the initiation of apoptosis. METHODS: Six-week-old male F344 rats were injected intraperitoneally with 20 mg/kg of doxorubicin or saline. Once the treatment was administered, both groups of animals were fasted with no food or water until sacrifice 24 h posttreatment. RESULTS: Doxorubicin treatment affected neither the proteome lysine acetylation status nor the expression of sirtuin 1, sirtuin 3, SOD1, or SOD2 in soleus of fasted animals. Doxorubicin treatment also did not affect the expression or activation of procaspase-1, procaspase-8, procaspase-9, or procaspase−12. CONCLUSION: We suggest that doxorubicin does not exert a direct effect on these catabolic parameters in skeletal muscle in vivo. |
format | Online Article Text |
id | pubmed-3774913 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-37749132013-09-17 The effects of acute doxorubicin treatment on proteome lysine acetylation status and apical caspases in skeletal muscle of fasted animals Dirks-Naylor, Amie J. Tran, Ngan T. K. Yang, Sendra Mabolo, Raean Kouzi, Samir A. J Cachexia Sarcopenia Muscle Original Article BACKGROUND: Doxorubicin treatment is known to cause muscular weakness. However, the cellular mechanisms have not been elucidated. We aimed to determine the effects of acute doxorubicin treatment on proteome lysine acetylation status, an indication of the apoptotic and inflammatory environment, and the expression and activation of various apical caspases involved in the initiation of apoptosis. METHODS: Six-week-old male F344 rats were injected intraperitoneally with 20 mg/kg of doxorubicin or saline. Once the treatment was administered, both groups of animals were fasted with no food or water until sacrifice 24 h posttreatment. RESULTS: Doxorubicin treatment affected neither the proteome lysine acetylation status nor the expression of sirtuin 1, sirtuin 3, SOD1, or SOD2 in soleus of fasted animals. Doxorubicin treatment also did not affect the expression or activation of procaspase-1, procaspase-8, procaspase-9, or procaspase−12. CONCLUSION: We suggest that doxorubicin does not exert a direct effect on these catabolic parameters in skeletal muscle in vivo. Springer Berlin Heidelberg 2013-03-26 2013-09 /pmc/articles/PMC3774913/ /pubmed/23529675 http://dx.doi.org/10.1007/s13539-013-0104-z Text en © Springer-Verlag Berlin Heidelberg 2013 |
spellingShingle | Original Article Dirks-Naylor, Amie J. Tran, Ngan T. K. Yang, Sendra Mabolo, Raean Kouzi, Samir A. The effects of acute doxorubicin treatment on proteome lysine acetylation status and apical caspases in skeletal muscle of fasted animals |
title | The effects of acute doxorubicin treatment on proteome lysine acetylation status and apical caspases in skeletal muscle of fasted animals |
title_full | The effects of acute doxorubicin treatment on proteome lysine acetylation status and apical caspases in skeletal muscle of fasted animals |
title_fullStr | The effects of acute doxorubicin treatment on proteome lysine acetylation status and apical caspases in skeletal muscle of fasted animals |
title_full_unstemmed | The effects of acute doxorubicin treatment on proteome lysine acetylation status and apical caspases in skeletal muscle of fasted animals |
title_short | The effects of acute doxorubicin treatment on proteome lysine acetylation status and apical caspases in skeletal muscle of fasted animals |
title_sort | effects of acute doxorubicin treatment on proteome lysine acetylation status and apical caspases in skeletal muscle of fasted animals |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3774913/ https://www.ncbi.nlm.nih.gov/pubmed/23529675 http://dx.doi.org/10.1007/s13539-013-0104-z |
work_keys_str_mv | AT dirksnayloramiej theeffectsofacutedoxorubicintreatmentonproteomelysineacetylationstatusandapicalcaspasesinskeletalmuscleoffastedanimals AT tranngantk theeffectsofacutedoxorubicintreatmentonproteomelysineacetylationstatusandapicalcaspasesinskeletalmuscleoffastedanimals AT yangsendra theeffectsofacutedoxorubicintreatmentonproteomelysineacetylationstatusandapicalcaspasesinskeletalmuscleoffastedanimals AT maboloraean theeffectsofacutedoxorubicintreatmentonproteomelysineacetylationstatusandapicalcaspasesinskeletalmuscleoffastedanimals AT kouzisamira theeffectsofacutedoxorubicintreatmentonproteomelysineacetylationstatusandapicalcaspasesinskeletalmuscleoffastedanimals AT dirksnayloramiej effectsofacutedoxorubicintreatmentonproteomelysineacetylationstatusandapicalcaspasesinskeletalmuscleoffastedanimals AT tranngantk effectsofacutedoxorubicintreatmentonproteomelysineacetylationstatusandapicalcaspasesinskeletalmuscleoffastedanimals AT yangsendra effectsofacutedoxorubicintreatmentonproteomelysineacetylationstatusandapicalcaspasesinskeletalmuscleoffastedanimals AT maboloraean effectsofacutedoxorubicintreatmentonproteomelysineacetylationstatusandapicalcaspasesinskeletalmuscleoffastedanimals AT kouzisamira effectsofacutedoxorubicintreatmentonproteomelysineacetylationstatusandapicalcaspasesinskeletalmuscleoffastedanimals |