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Bisdemethoxycurcumin Increases Sirt1 to Antagonize t-BHP-Induced Premature Senescence in WI38 Fibroblast Cells
Curcuminoids are well known for their capabilities to combat risk factors that are associated with ageing and cellular senescence. Recent reports have demonstrated that curcuminoids can extend the lifespan of model organisms. However, the underlying mechanisms by which these polyphenic compounds exe...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3775445/ https://www.ncbi.nlm.nih.gov/pubmed/24078830 http://dx.doi.org/10.1155/2013/851714 |
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author | Li, Ying-Bo Zhong, Zhang-Feng Chen, Mei-Wan Bao, Jiao-Lin Wu, Guo-Sheng Zhang, Qing-Wen Lee, Simon Ming-Yuen Hoi, Pui-Man Wang, Yi-Tao |
author_facet | Li, Ying-Bo Zhong, Zhang-Feng Chen, Mei-Wan Bao, Jiao-Lin Wu, Guo-Sheng Zhang, Qing-Wen Lee, Simon Ming-Yuen Hoi, Pui-Man Wang, Yi-Tao |
author_sort | Li, Ying-Bo |
collection | PubMed |
description | Curcuminoids are well known for their capabilities to combat risk factors that are associated with ageing and cellular senescence. Recent reports have demonstrated that curcuminoids can extend the lifespan of model organisms. However, the underlying mechanisms by which these polyphenic compounds exert these beneficial effects remain unknown. In this study, t-BHP-induced premature senescence model in human fibroblasts was chosen to explore the protective effects of a curcuminoid, bisdemethoxycurcumin (BDMC), on cellular senescence. The results demonstrated that BDMC attenuated oxidative stress-induced senescence-like features which include the induction of an enlarged cellular appearance, higher frequency of senescence-associated β-galactosidase staining activity, appearance of senescence-associated heterochromatic foci in nuclei, decrease in proliferation capability, and alteration in related molecules such as p16 and retinoblastoma protein. Notably, we found that BDMC treatment activated Sirt1/AMPK signaling pathway. Moreover, downregulating Sirt1 by the pharmacological inhibitor nicotianamine or small interfering RNA blocked BDMC-mediated protection against t-BHP-mediated decrease in proliferation. These results suggested that BDMC prevented t-BHP-induced cellular senescence, and BDMC-induced Sirt1 may be a mechanism mediating its beneficial effects. |
format | Online Article Text |
id | pubmed-3775445 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-37754452013-09-29 Bisdemethoxycurcumin Increases Sirt1 to Antagonize t-BHP-Induced Premature Senescence in WI38 Fibroblast Cells Li, Ying-Bo Zhong, Zhang-Feng Chen, Mei-Wan Bao, Jiao-Lin Wu, Guo-Sheng Zhang, Qing-Wen Lee, Simon Ming-Yuen Hoi, Pui-Man Wang, Yi-Tao Evid Based Complement Alternat Med Research Article Curcuminoids are well known for their capabilities to combat risk factors that are associated with ageing and cellular senescence. Recent reports have demonstrated that curcuminoids can extend the lifespan of model organisms. However, the underlying mechanisms by which these polyphenic compounds exert these beneficial effects remain unknown. In this study, t-BHP-induced premature senescence model in human fibroblasts was chosen to explore the protective effects of a curcuminoid, bisdemethoxycurcumin (BDMC), on cellular senescence. The results demonstrated that BDMC attenuated oxidative stress-induced senescence-like features which include the induction of an enlarged cellular appearance, higher frequency of senescence-associated β-galactosidase staining activity, appearance of senescence-associated heterochromatic foci in nuclei, decrease in proliferation capability, and alteration in related molecules such as p16 and retinoblastoma protein. Notably, we found that BDMC treatment activated Sirt1/AMPK signaling pathway. Moreover, downregulating Sirt1 by the pharmacological inhibitor nicotianamine or small interfering RNA blocked BDMC-mediated protection against t-BHP-mediated decrease in proliferation. These results suggested that BDMC prevented t-BHP-induced cellular senescence, and BDMC-induced Sirt1 may be a mechanism mediating its beneficial effects. Hindawi Publishing Corporation 2013 2013-09-02 /pmc/articles/PMC3775445/ /pubmed/24078830 http://dx.doi.org/10.1155/2013/851714 Text en Copyright © 2013 Ying-Bo Li et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Li, Ying-Bo Zhong, Zhang-Feng Chen, Mei-Wan Bao, Jiao-Lin Wu, Guo-Sheng Zhang, Qing-Wen Lee, Simon Ming-Yuen Hoi, Pui-Man Wang, Yi-Tao Bisdemethoxycurcumin Increases Sirt1 to Antagonize t-BHP-Induced Premature Senescence in WI38 Fibroblast Cells |
title | Bisdemethoxycurcumin Increases Sirt1 to Antagonize t-BHP-Induced Premature Senescence in WI38 Fibroblast Cells |
title_full | Bisdemethoxycurcumin Increases Sirt1 to Antagonize t-BHP-Induced Premature Senescence in WI38 Fibroblast Cells |
title_fullStr | Bisdemethoxycurcumin Increases Sirt1 to Antagonize t-BHP-Induced Premature Senescence in WI38 Fibroblast Cells |
title_full_unstemmed | Bisdemethoxycurcumin Increases Sirt1 to Antagonize t-BHP-Induced Premature Senescence in WI38 Fibroblast Cells |
title_short | Bisdemethoxycurcumin Increases Sirt1 to Antagonize t-BHP-Induced Premature Senescence in WI38 Fibroblast Cells |
title_sort | bisdemethoxycurcumin increases sirt1 to antagonize t-bhp-induced premature senescence in wi38 fibroblast cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3775445/ https://www.ncbi.nlm.nih.gov/pubmed/24078830 http://dx.doi.org/10.1155/2013/851714 |
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