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Multilocus Sequence Typing of Borrelia burgdorferi Suggests Existence of Lineages with Differential Pathogenic Properties in Humans

The clinical manifestations of Lyme disease, caused by Borrelia burgdorferi, vary considerably in different patients, possibly due to infection by strains with varying pathogenicity. Both rRNA intergenic spacer and ospC typing methods have proven to be useful tools for categorizing B. burgdorferi st...

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Autores principales: Hanincova, Klara, Mukherjee, Priyanka, Ogden, Nicholas H., Margos, Gabriele, Wormser, Gary P., Reed, Kurt D., Meece, Jennifer K., Vandermause, Mary F., Schwartz, Ira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3775742/
https://www.ncbi.nlm.nih.gov/pubmed/24069170
http://dx.doi.org/10.1371/journal.pone.0073066
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author Hanincova, Klara
Mukherjee, Priyanka
Ogden, Nicholas H.
Margos, Gabriele
Wormser, Gary P.
Reed, Kurt D.
Meece, Jennifer K.
Vandermause, Mary F.
Schwartz, Ira
author_facet Hanincova, Klara
Mukherjee, Priyanka
Ogden, Nicholas H.
Margos, Gabriele
Wormser, Gary P.
Reed, Kurt D.
Meece, Jennifer K.
Vandermause, Mary F.
Schwartz, Ira
author_sort Hanincova, Klara
collection PubMed
description The clinical manifestations of Lyme disease, caused by Borrelia burgdorferi, vary considerably in different patients, possibly due to infection by strains with varying pathogenicity. Both rRNA intergenic spacer and ospC typing methods have proven to be useful tools for categorizing B. burgdorferi strains that vary in their tendency to disseminate in humans. Neither method, however, is suitable for inferring intraspecific relationships among strains that are important for understanding the evolution of pathogenicity and the geographic spread of disease. In this study, multilocus sequence typing (MLST) was employed to investigate the population structure of B. burgdorferi recovered from human Lyme disease patients. A total of 146 clinical isolates from patients in New York and Wisconsin were divided into 53 sequence types (STs). A goeBURST analysis, that also included previously published STs from the northeastern and upper Midwestern US and adjoining areas of Canada, identified 11 major and 3 minor clonal complexes, as well as 14 singletons. The data revealed that patients from New York and Wisconsin were infected with two distinct, but genetically and phylogenetically closely related, populations of B. burgdorferi. Importantly, the data suggest the existence of B. burgdorferi lineages with differential capabilities for dissemination in humans. Interestingly, the data also indicate that MLST is better able to predict the outcome of localized or disseminated infection than is ospC typing.
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spelling pubmed-37757422013-09-25 Multilocus Sequence Typing of Borrelia burgdorferi Suggests Existence of Lineages with Differential Pathogenic Properties in Humans Hanincova, Klara Mukherjee, Priyanka Ogden, Nicholas H. Margos, Gabriele Wormser, Gary P. Reed, Kurt D. Meece, Jennifer K. Vandermause, Mary F. Schwartz, Ira PLoS One Research Article The clinical manifestations of Lyme disease, caused by Borrelia burgdorferi, vary considerably in different patients, possibly due to infection by strains with varying pathogenicity. Both rRNA intergenic spacer and ospC typing methods have proven to be useful tools for categorizing B. burgdorferi strains that vary in their tendency to disseminate in humans. Neither method, however, is suitable for inferring intraspecific relationships among strains that are important for understanding the evolution of pathogenicity and the geographic spread of disease. In this study, multilocus sequence typing (MLST) was employed to investigate the population structure of B. burgdorferi recovered from human Lyme disease patients. A total of 146 clinical isolates from patients in New York and Wisconsin were divided into 53 sequence types (STs). A goeBURST analysis, that also included previously published STs from the northeastern and upper Midwestern US and adjoining areas of Canada, identified 11 major and 3 minor clonal complexes, as well as 14 singletons. The data revealed that patients from New York and Wisconsin were infected with two distinct, but genetically and phylogenetically closely related, populations of B. burgdorferi. Importantly, the data suggest the existence of B. burgdorferi lineages with differential capabilities for dissemination in humans. Interestingly, the data also indicate that MLST is better able to predict the outcome of localized or disseminated infection than is ospC typing. Public Library of Science 2013-09-17 /pmc/articles/PMC3775742/ /pubmed/24069170 http://dx.doi.org/10.1371/journal.pone.0073066 Text en © 2013 Hanincova et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Hanincova, Klara
Mukherjee, Priyanka
Ogden, Nicholas H.
Margos, Gabriele
Wormser, Gary P.
Reed, Kurt D.
Meece, Jennifer K.
Vandermause, Mary F.
Schwartz, Ira
Multilocus Sequence Typing of Borrelia burgdorferi Suggests Existence of Lineages with Differential Pathogenic Properties in Humans
title Multilocus Sequence Typing of Borrelia burgdorferi Suggests Existence of Lineages with Differential Pathogenic Properties in Humans
title_full Multilocus Sequence Typing of Borrelia burgdorferi Suggests Existence of Lineages with Differential Pathogenic Properties in Humans
title_fullStr Multilocus Sequence Typing of Borrelia burgdorferi Suggests Existence of Lineages with Differential Pathogenic Properties in Humans
title_full_unstemmed Multilocus Sequence Typing of Borrelia burgdorferi Suggests Existence of Lineages with Differential Pathogenic Properties in Humans
title_short Multilocus Sequence Typing of Borrelia burgdorferi Suggests Existence of Lineages with Differential Pathogenic Properties in Humans
title_sort multilocus sequence typing of borrelia burgdorferi suggests existence of lineages with differential pathogenic properties in humans
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3775742/
https://www.ncbi.nlm.nih.gov/pubmed/24069170
http://dx.doi.org/10.1371/journal.pone.0073066
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