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Effect of an angiotensin II type 1 receptor blocker on caveolin-1 expression in prostate cancer cells

INTRODUCTION: Caveolin-1, the major structural protein of caveolae, interacts directly with the AT1 receptor. The biological functions of caveolin-1 in cancer are compound, multifaceted, and depend on cell type, tumour grade and cancer stage. The AT1-R-caveolin complex in caveolae may coordinate ang...

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Autores principales: Piastowska-Ciesielska, Agnieszka Wanda, Kozłowski, Marcin, Wagner, Waldemar, Domińska, Kamila, Ochędalski, Tomasz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Termedia Publishing House 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3776164/
https://www.ncbi.nlm.nih.gov/pubmed/24049538
http://dx.doi.org/10.5114/aoms.2012.30955
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author Piastowska-Ciesielska, Agnieszka Wanda
Kozłowski, Marcin
Wagner, Waldemar
Domińska, Kamila
Ochędalski, Tomasz
author_facet Piastowska-Ciesielska, Agnieszka Wanda
Kozłowski, Marcin
Wagner, Waldemar
Domińska, Kamila
Ochędalski, Tomasz
author_sort Piastowska-Ciesielska, Agnieszka Wanda
collection PubMed
description INTRODUCTION: Caveolin-1, the major structural protein of caveolae, interacts directly with the AT1 receptor. The biological functions of caveolin-1 in cancer are compound, multifaceted, and depend on cell type, tumour grade and cancer stage. The AT1-R-caveolin complex in caveolae may coordinate angiotensin II (Ang II) induced signalling. The aim of this study was to determine the effect of the angiotensin II receptor type 1 blocker candesartan on caveolin expression in human metastatic prostate adenocarcinoma cells PC-3. MATERIAL AND METHODS: WST-1 and BrdU assays were used as indicators of cell viability and proliferation after angiotensin II and/or candesartan stimulation. Real-time RT–PCR and western blot were used to study the effect of Ang II and/or candesartan on the expression of Cav-1 and AT1-R in PC-3 cells RESULTS: We found that the expression of caveolin-1 mRNA in the PC-3 cells treated with CV was significantly decreased in comparison with the control (2.9 ±0.17, 4.7 ±0.6, p < 0.05), whereas a higher caveolin-1 mRNA expression was observed in those after Ang II treatment (6.0 ±0.43, 4.7 ±0.6, p < 0.05). Protein analysis indicate that the expression of caveolin-1 protein in the PC-3 cells treated with candesartan was significantly decreased when compared with the control (0.69 ±0.05, 1.6 ±0.12, p < 0.05), whereas higher caveolin-1 protein expression was observed after Ang II treatment (2.5 ±0.20, 1.6 ±0.12, p < 0.05). CONCLUSIONS: These results provide new information on the action of candesartan and may improve the knowledge about AT1 receptor inhibitors, which can be potentially useful in prostate cancer therapy.
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spelling pubmed-37761642013-09-18 Effect of an angiotensin II type 1 receptor blocker on caveolin-1 expression in prostate cancer cells Piastowska-Ciesielska, Agnieszka Wanda Kozłowski, Marcin Wagner, Waldemar Domińska, Kamila Ochędalski, Tomasz Arch Med Sci Basic Research INTRODUCTION: Caveolin-1, the major structural protein of caveolae, interacts directly with the AT1 receptor. The biological functions of caveolin-1 in cancer are compound, multifaceted, and depend on cell type, tumour grade and cancer stage. The AT1-R-caveolin complex in caveolae may coordinate angiotensin II (Ang II) induced signalling. The aim of this study was to determine the effect of the angiotensin II receptor type 1 blocker candesartan on caveolin expression in human metastatic prostate adenocarcinoma cells PC-3. MATERIAL AND METHODS: WST-1 and BrdU assays were used as indicators of cell viability and proliferation after angiotensin II and/or candesartan stimulation. Real-time RT–PCR and western blot were used to study the effect of Ang II and/or candesartan on the expression of Cav-1 and AT1-R in PC-3 cells RESULTS: We found that the expression of caveolin-1 mRNA in the PC-3 cells treated with CV was significantly decreased in comparison with the control (2.9 ±0.17, 4.7 ±0.6, p < 0.05), whereas a higher caveolin-1 mRNA expression was observed in those after Ang II treatment (6.0 ±0.43, 4.7 ±0.6, p < 0.05). Protein analysis indicate that the expression of caveolin-1 protein in the PC-3 cells treated with candesartan was significantly decreased when compared with the control (0.69 ±0.05, 1.6 ±0.12, p < 0.05), whereas higher caveolin-1 protein expression was observed after Ang II treatment (2.5 ±0.20, 1.6 ±0.12, p < 0.05). CONCLUSIONS: These results provide new information on the action of candesartan and may improve the knowledge about AT1 receptor inhibitors, which can be potentially useful in prostate cancer therapy. Termedia Publishing House 2012-10-08 2013-08-30 /pmc/articles/PMC3776164/ /pubmed/24049538 http://dx.doi.org/10.5114/aoms.2012.30955 Text en Copyright © 2013 Termedia & Banach http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 3.0 Unported License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Basic Research
Piastowska-Ciesielska, Agnieszka Wanda
Kozłowski, Marcin
Wagner, Waldemar
Domińska, Kamila
Ochędalski, Tomasz
Effect of an angiotensin II type 1 receptor blocker on caveolin-1 expression in prostate cancer cells
title Effect of an angiotensin II type 1 receptor blocker on caveolin-1 expression in prostate cancer cells
title_full Effect of an angiotensin II type 1 receptor blocker on caveolin-1 expression in prostate cancer cells
title_fullStr Effect of an angiotensin II type 1 receptor blocker on caveolin-1 expression in prostate cancer cells
title_full_unstemmed Effect of an angiotensin II type 1 receptor blocker on caveolin-1 expression in prostate cancer cells
title_short Effect of an angiotensin II type 1 receptor blocker on caveolin-1 expression in prostate cancer cells
title_sort effect of an angiotensin ii type 1 receptor blocker on caveolin-1 expression in prostate cancer cells
topic Basic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3776164/
https://www.ncbi.nlm.nih.gov/pubmed/24049538
http://dx.doi.org/10.5114/aoms.2012.30955
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