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The elastic properties of single double-stranded DNA chains of different lengths as measured with optical tweezers

Optical tweezers are microscopic tools with extraordinary precision in the determination of the position (±2 nm) of a colloid (diameter: ∼2.0 μm) in 3D-space and in the measurement of small forces in the range between 0.1 and 100 pN (pN=10(−12) N). Experiments are reported in which single double-str...

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Detalles Bibliográficos
Autores principales: Salomo, M., Kegler, K., Gutsche, C., Struhalla, M., Reinmuth, J., Skokow, W., Hahn, U., Kremer, F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3776250/
https://www.ncbi.nlm.nih.gov/pubmed/24058243
http://dx.doi.org/10.1007/s00396-006-1517-4
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author Salomo, M.
Kegler, K.
Gutsche, C.
Struhalla, M.
Reinmuth, J.
Skokow, W.
Hahn, U.
Kremer, F.
author_facet Salomo, M.
Kegler, K.
Gutsche, C.
Struhalla, M.
Reinmuth, J.
Skokow, W.
Hahn, U.
Kremer, F.
author_sort Salomo, M.
collection PubMed
description Optical tweezers are microscopic tools with extraordinary precision in the determination of the position (±2 nm) of a colloid (diameter: ∼2.0 μm) in 3D-space and in the measurement of small forces in the range between 0.1 and 100 pN (pN=10(−12) N). Experiments are reported in which single double-stranded (ds)-DNA chains of different length [2,000 base pairs (bp), 3,000, 4,000, and 6,000 bp] are spanned between two colloidal particles by use of appropriate molecular linkers. For the forces applied (≤40 pN) a fully reversible and well reproducible force–extension dependence is found. The data can be well described by both the worm-like chain model or by an approach developed by R. G. Winkler. For the resulting persistence length, a pronounced dependence on the ionic concentration in the surrounding medium is found.
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spelling pubmed-37762502013-09-25 The elastic properties of single double-stranded DNA chains of different lengths as measured with optical tweezers Salomo, M. Kegler, K. Gutsche, C. Struhalla, M. Reinmuth, J. Skokow, W. Hahn, U. Kremer, F. Colloid Polym Sci Original Contribution Optical tweezers are microscopic tools with extraordinary precision in the determination of the position (±2 nm) of a colloid (diameter: ∼2.0 μm) in 3D-space and in the measurement of small forces in the range between 0.1 and 100 pN (pN=10(−12) N). Experiments are reported in which single double-stranded (ds)-DNA chains of different length [2,000 base pairs (bp), 3,000, 4,000, and 6,000 bp] are spanned between two colloidal particles by use of appropriate molecular linkers. For the forces applied (≤40 pN) a fully reversible and well reproducible force–extension dependence is found. The data can be well described by both the worm-like chain model or by an approach developed by R. G. Winkler. For the resulting persistence length, a pronounced dependence on the ionic concentration in the surrounding medium is found. Springer-Verlag 2006-05-25 2006 /pmc/articles/PMC3776250/ /pubmed/24058243 http://dx.doi.org/10.1007/s00396-006-1517-4 Text en © Springer-Verlag 2006
spellingShingle Original Contribution
Salomo, M.
Kegler, K.
Gutsche, C.
Struhalla, M.
Reinmuth, J.
Skokow, W.
Hahn, U.
Kremer, F.
The elastic properties of single double-stranded DNA chains of different lengths as measured with optical tweezers
title The elastic properties of single double-stranded DNA chains of different lengths as measured with optical tweezers
title_full The elastic properties of single double-stranded DNA chains of different lengths as measured with optical tweezers
title_fullStr The elastic properties of single double-stranded DNA chains of different lengths as measured with optical tweezers
title_full_unstemmed The elastic properties of single double-stranded DNA chains of different lengths as measured with optical tweezers
title_short The elastic properties of single double-stranded DNA chains of different lengths as measured with optical tweezers
title_sort elastic properties of single double-stranded dna chains of different lengths as measured with optical tweezers
topic Original Contribution
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3776250/
https://www.ncbi.nlm.nih.gov/pubmed/24058243
http://dx.doi.org/10.1007/s00396-006-1517-4
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