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The elastic properties of single double-stranded DNA chains of different lengths as measured with optical tweezers
Optical tweezers are microscopic tools with extraordinary precision in the determination of the position (±2 nm) of a colloid (diameter: ∼2.0 μm) in 3D-space and in the measurement of small forces in the range between 0.1 and 100 pN (pN=10(−12) N). Experiments are reported in which single double-str...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer-Verlag
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3776250/ https://www.ncbi.nlm.nih.gov/pubmed/24058243 http://dx.doi.org/10.1007/s00396-006-1517-4 |
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author | Salomo, M. Kegler, K. Gutsche, C. Struhalla, M. Reinmuth, J. Skokow, W. Hahn, U. Kremer, F. |
author_facet | Salomo, M. Kegler, K. Gutsche, C. Struhalla, M. Reinmuth, J. Skokow, W. Hahn, U. Kremer, F. |
author_sort | Salomo, M. |
collection | PubMed |
description | Optical tweezers are microscopic tools with extraordinary precision in the determination of the position (±2 nm) of a colloid (diameter: ∼2.0 μm) in 3D-space and in the measurement of small forces in the range between 0.1 and 100 pN (pN=10(−12) N). Experiments are reported in which single double-stranded (ds)-DNA chains of different length [2,000 base pairs (bp), 3,000, 4,000, and 6,000 bp] are spanned between two colloidal particles by use of appropriate molecular linkers. For the forces applied (≤40 pN) a fully reversible and well reproducible force–extension dependence is found. The data can be well described by both the worm-like chain model or by an approach developed by R. G. Winkler. For the resulting persistence length, a pronounced dependence on the ionic concentration in the surrounding medium is found. |
format | Online Article Text |
id | pubmed-3776250 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | Springer-Verlag |
record_format | MEDLINE/PubMed |
spelling | pubmed-37762502013-09-25 The elastic properties of single double-stranded DNA chains of different lengths as measured with optical tweezers Salomo, M. Kegler, K. Gutsche, C. Struhalla, M. Reinmuth, J. Skokow, W. Hahn, U. Kremer, F. Colloid Polym Sci Original Contribution Optical tweezers are microscopic tools with extraordinary precision in the determination of the position (±2 nm) of a colloid (diameter: ∼2.0 μm) in 3D-space and in the measurement of small forces in the range between 0.1 and 100 pN (pN=10(−12) N). Experiments are reported in which single double-stranded (ds)-DNA chains of different length [2,000 base pairs (bp), 3,000, 4,000, and 6,000 bp] are spanned between two colloidal particles by use of appropriate molecular linkers. For the forces applied (≤40 pN) a fully reversible and well reproducible force–extension dependence is found. The data can be well described by both the worm-like chain model or by an approach developed by R. G. Winkler. For the resulting persistence length, a pronounced dependence on the ionic concentration in the surrounding medium is found. Springer-Verlag 2006-05-25 2006 /pmc/articles/PMC3776250/ /pubmed/24058243 http://dx.doi.org/10.1007/s00396-006-1517-4 Text en © Springer-Verlag 2006 |
spellingShingle | Original Contribution Salomo, M. Kegler, K. Gutsche, C. Struhalla, M. Reinmuth, J. Skokow, W. Hahn, U. Kremer, F. The elastic properties of single double-stranded DNA chains of different lengths as measured with optical tweezers |
title | The elastic properties of single double-stranded DNA chains of different lengths as measured with optical tweezers |
title_full | The elastic properties of single double-stranded DNA chains of different lengths as measured with optical tweezers |
title_fullStr | The elastic properties of single double-stranded DNA chains of different lengths as measured with optical tweezers |
title_full_unstemmed | The elastic properties of single double-stranded DNA chains of different lengths as measured with optical tweezers |
title_short | The elastic properties of single double-stranded DNA chains of different lengths as measured with optical tweezers |
title_sort | elastic properties of single double-stranded dna chains of different lengths as measured with optical tweezers |
topic | Original Contribution |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3776250/ https://www.ncbi.nlm.nih.gov/pubmed/24058243 http://dx.doi.org/10.1007/s00396-006-1517-4 |
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