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M2-like macrophages are responsible for collagen degradation through a mannose receptor–mediated pathway

Tissue remodeling processes critically depend on the timely removal and remodeling of preexisting collagen scaffolds. Nevertheless, many aspects related to the turnover of this abundant extracellular matrix component in vivo are still incompletely understood. We therefore took advantage of recent ad...

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Autores principales: Madsen, Daniel H., Leonard, Daniel, Masedunskas, Andrius, Moyer, Amanda, Jürgensen, Henrik Jessen, Peters, Diane E., Amornphimoltham, Panomwat, Selvaraj, Arul, Yamada, Susan S., Brenner, David A., Burgdorf, Sven, Engelholm, Lars H., Behrendt, Niels, Holmbeck, Kenn, Weigert, Roberto, Bugge, Thomas H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3776354/
https://www.ncbi.nlm.nih.gov/pubmed/24019537
http://dx.doi.org/10.1083/jcb.201301081
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author Madsen, Daniel H.
Leonard, Daniel
Masedunskas, Andrius
Moyer, Amanda
Jürgensen, Henrik Jessen
Peters, Diane E.
Amornphimoltham, Panomwat
Selvaraj, Arul
Yamada, Susan S.
Brenner, David A.
Burgdorf, Sven
Engelholm, Lars H.
Behrendt, Niels
Holmbeck, Kenn
Weigert, Roberto
Bugge, Thomas H.
author_facet Madsen, Daniel H.
Leonard, Daniel
Masedunskas, Andrius
Moyer, Amanda
Jürgensen, Henrik Jessen
Peters, Diane E.
Amornphimoltham, Panomwat
Selvaraj, Arul
Yamada, Susan S.
Brenner, David A.
Burgdorf, Sven
Engelholm, Lars H.
Behrendt, Niels
Holmbeck, Kenn
Weigert, Roberto
Bugge, Thomas H.
author_sort Madsen, Daniel H.
collection PubMed
description Tissue remodeling processes critically depend on the timely removal and remodeling of preexisting collagen scaffolds. Nevertheless, many aspects related to the turnover of this abundant extracellular matrix component in vivo are still incompletely understood. We therefore took advantage of recent advances in optical imaging to develop an assay to visualize collagen turnover in situ and identify cell types and molecules involved in this process. Collagen introduced into the dermis of mice underwent cellular endocytosis in a partially matrix metalloproteinase–dependent manner and was subsequently routed to lysosomes for complete degradation. Collagen uptake was predominantly executed by a quantitatively minor population of M2-like macrophages, whereas more abundant Col1a1-expressing fibroblasts and Cx3cr1-expressing macrophages internalized collagen at lower levels. Genetic ablation of the collagen receptors mannose receptor (Mrc1) and urokinase plasminogen activator receptor–associated protein (Endo180 and Mrc2) impaired this intracellular collagen degradation pathway. This study demonstrates the importance of receptor-mediated cellular uptake to collagen turnover in vivo and identifies a key role of M2-like macrophages in this process.
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spelling pubmed-37763542014-03-16 M2-like macrophages are responsible for collagen degradation through a mannose receptor–mediated pathway Madsen, Daniel H. Leonard, Daniel Masedunskas, Andrius Moyer, Amanda Jürgensen, Henrik Jessen Peters, Diane E. Amornphimoltham, Panomwat Selvaraj, Arul Yamada, Susan S. Brenner, David A. Burgdorf, Sven Engelholm, Lars H. Behrendt, Niels Holmbeck, Kenn Weigert, Roberto Bugge, Thomas H. J Cell Biol Research Articles Tissue remodeling processes critically depend on the timely removal and remodeling of preexisting collagen scaffolds. Nevertheless, many aspects related to the turnover of this abundant extracellular matrix component in vivo are still incompletely understood. We therefore took advantage of recent advances in optical imaging to develop an assay to visualize collagen turnover in situ and identify cell types and molecules involved in this process. Collagen introduced into the dermis of mice underwent cellular endocytosis in a partially matrix metalloproteinase–dependent manner and was subsequently routed to lysosomes for complete degradation. Collagen uptake was predominantly executed by a quantitatively minor population of M2-like macrophages, whereas more abundant Col1a1-expressing fibroblasts and Cx3cr1-expressing macrophages internalized collagen at lower levels. Genetic ablation of the collagen receptors mannose receptor (Mrc1) and urokinase plasminogen activator receptor–associated protein (Endo180 and Mrc2) impaired this intracellular collagen degradation pathway. This study demonstrates the importance of receptor-mediated cellular uptake to collagen turnover in vivo and identifies a key role of M2-like macrophages in this process. The Rockefeller University Press 2013-09-16 /pmc/articles/PMC3776354/ /pubmed/24019537 http://dx.doi.org/10.1083/jcb.201301081 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Madsen, Daniel H.
Leonard, Daniel
Masedunskas, Andrius
Moyer, Amanda
Jürgensen, Henrik Jessen
Peters, Diane E.
Amornphimoltham, Panomwat
Selvaraj, Arul
Yamada, Susan S.
Brenner, David A.
Burgdorf, Sven
Engelholm, Lars H.
Behrendt, Niels
Holmbeck, Kenn
Weigert, Roberto
Bugge, Thomas H.
M2-like macrophages are responsible for collagen degradation through a mannose receptor–mediated pathway
title M2-like macrophages are responsible for collagen degradation through a mannose receptor–mediated pathway
title_full M2-like macrophages are responsible for collagen degradation through a mannose receptor–mediated pathway
title_fullStr M2-like macrophages are responsible for collagen degradation through a mannose receptor–mediated pathway
title_full_unstemmed M2-like macrophages are responsible for collagen degradation through a mannose receptor–mediated pathway
title_short M2-like macrophages are responsible for collagen degradation through a mannose receptor–mediated pathway
title_sort m2-like macrophages are responsible for collagen degradation through a mannose receptor–mediated pathway
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3776354/
https://www.ncbi.nlm.nih.gov/pubmed/24019537
http://dx.doi.org/10.1083/jcb.201301081
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