Molecular Detection of Peripheral Blood Breast Cancer mRNA Transcripts as a Surrogate Biomarker for Circulating Tumor Cells

Circulating tumor cells (CTCs) are becoming a scientifically recognized indicator of primary tumors and/or metastasis. These cells can now be accurately detected and characterized as the result of technological advances. We analyzed the presence of CTCs in the peripheral blood of patients with metas...

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Autores principales: Lasa, Adriana, Garcia, Arnal, Alonso, Carmen, Millet, Pilar, Cornet, Mónica, Cajal, Teresa Ramón y, Baiget, Montserrat, Barnadas, Agusti
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3776801/
https://www.ncbi.nlm.nih.gov/pubmed/24058517
http://dx.doi.org/10.1371/journal.pone.0074079
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author Lasa, Adriana
Garcia, Arnal
Alonso, Carmen
Millet, Pilar
Cornet, Mónica
Cajal, Teresa Ramón y
Baiget, Montserrat
Barnadas, Agusti
author_facet Lasa, Adriana
Garcia, Arnal
Alonso, Carmen
Millet, Pilar
Cornet, Mónica
Cajal, Teresa Ramón y
Baiget, Montserrat
Barnadas, Agusti
author_sort Lasa, Adriana
collection PubMed
description Circulating tumor cells (CTCs) are becoming a scientifically recognized indicator of primary tumors and/or metastasis. These cells can now be accurately detected and characterized as the result of technological advances. We analyzed the presence of CTCs in the peripheral blood of patients with metastatic breast cancer by real-time reverse-transcription PCR (RT-qPCR) using a panel of selected genes. The analysis of a single marker, without an EpCAM based enrichment approach, allowed the positive identification of 35% of the metastatic breast cancer patients. The analysis of five genes (SCGB2, TFF1, TFF3, Muc1, KRT20) performed in all the samples increased the detection to 61%. We describe a sensitive, reproducible and easy to implement approach to characterize CTC in patients with metastasic breast cancer.
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spelling pubmed-37768012013-09-20 Molecular Detection of Peripheral Blood Breast Cancer mRNA Transcripts as a Surrogate Biomarker for Circulating Tumor Cells Lasa, Adriana Garcia, Arnal Alonso, Carmen Millet, Pilar Cornet, Mónica Cajal, Teresa Ramón y Baiget, Montserrat Barnadas, Agusti PLoS One Research Article Circulating tumor cells (CTCs) are becoming a scientifically recognized indicator of primary tumors and/or metastasis. These cells can now be accurately detected and characterized as the result of technological advances. We analyzed the presence of CTCs in the peripheral blood of patients with metastatic breast cancer by real-time reverse-transcription PCR (RT-qPCR) using a panel of selected genes. The analysis of a single marker, without an EpCAM based enrichment approach, allowed the positive identification of 35% of the metastatic breast cancer patients. The analysis of five genes (SCGB2, TFF1, TFF3, Muc1, KRT20) performed in all the samples increased the detection to 61%. We describe a sensitive, reproducible and easy to implement approach to characterize CTC in patients with metastasic breast cancer. Public Library of Science 2013-09-18 /pmc/articles/PMC3776801/ /pubmed/24058517 http://dx.doi.org/10.1371/journal.pone.0074079 Text en © 2013 Lasa et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Lasa, Adriana
Garcia, Arnal
Alonso, Carmen
Millet, Pilar
Cornet, Mónica
Cajal, Teresa Ramón y
Baiget, Montserrat
Barnadas, Agusti
Molecular Detection of Peripheral Blood Breast Cancer mRNA Transcripts as a Surrogate Biomarker for Circulating Tumor Cells
title Molecular Detection of Peripheral Blood Breast Cancer mRNA Transcripts as a Surrogate Biomarker for Circulating Tumor Cells
title_full Molecular Detection of Peripheral Blood Breast Cancer mRNA Transcripts as a Surrogate Biomarker for Circulating Tumor Cells
title_fullStr Molecular Detection of Peripheral Blood Breast Cancer mRNA Transcripts as a Surrogate Biomarker for Circulating Tumor Cells
title_full_unstemmed Molecular Detection of Peripheral Blood Breast Cancer mRNA Transcripts as a Surrogate Biomarker for Circulating Tumor Cells
title_short Molecular Detection of Peripheral Blood Breast Cancer mRNA Transcripts as a Surrogate Biomarker for Circulating Tumor Cells
title_sort molecular detection of peripheral blood breast cancer mrna transcripts as a surrogate biomarker for circulating tumor cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3776801/
https://www.ncbi.nlm.nih.gov/pubmed/24058517
http://dx.doi.org/10.1371/journal.pone.0074079
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