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P2Y2 receptor promotes cell invasion and metastasis in prostate cancer cells

BACKGROUND: Our previous study demonstrated that extracellular adenosine 5′-triphosphate (ATP) stimulated prostate cancer cell invasion via P2Y receptors. However, the purinergic receptor subtype(s) involved in this process remains unclear. Here we aimed to determine whether P2Y2, one subtype of P2Y...

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Autores principales: Li, W-H, Qiu, Y, Zhang, H-Q, Liu, Y, You, J-F, Tian, X-X, Fang, W-G
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3776994/
https://www.ncbi.nlm.nih.gov/pubmed/23969730
http://dx.doi.org/10.1038/bjc.2013.484
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author Li, W-H
Qiu, Y
Zhang, H-Q
Liu, Y
You, J-F
Tian, X-X
Fang, W-G
author_facet Li, W-H
Qiu, Y
Zhang, H-Q
Liu, Y
You, J-F
Tian, X-X
Fang, W-G
author_sort Li, W-H
collection PubMed
description BACKGROUND: Our previous study demonstrated that extracellular adenosine 5′-triphosphate (ATP) stimulated prostate cancer cell invasion via P2Y receptors. However, the purinergic receptor subtype(s) involved in this process remains unclear. Here we aimed to determine whether P2Y2, one subtype of P2Y receptors, was involved in the invasion and metastasis of prostate cancer cells, and elucidated the underlying mechanism. METHODS: RNAi was introduced to silence the expression of P2Y2. In vitro invasion and migration assays and in vivo experiments were carried out to examine the role of P2Y2 receptor in cell invasion and metastasis. cDNA microarray was performed to identify the differentially expressed genes downstream of ATP treatment. RESULTS: P2Y2 was significantly expressed in the prostate cancer cells. Knockdown of P2Y2 receptor suppressed cell invasion and metastasis in vitro and in vivo. Further experiments identified that ATP could promote IL-8 and Snail expression and inhibit E-cadherin and Claudin-1 expression. Knockdown of P2Y2 receptor affected the expression of these EMT/invasion-related genes in vitro and in vivo. CONCLUSION: P2Y2 receptor promotes cell invasion and metastasis in prostate cancer cells via some EMT/invasion-related genes. Thereby, P2Y2 receptor could be a potential therapeutic target for the treatment of prostate cancer.
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spelling pubmed-37769942014-09-17 P2Y2 receptor promotes cell invasion and metastasis in prostate cancer cells Li, W-H Qiu, Y Zhang, H-Q Liu, Y You, J-F Tian, X-X Fang, W-G Br J Cancer Molecular Diagnostics BACKGROUND: Our previous study demonstrated that extracellular adenosine 5′-triphosphate (ATP) stimulated prostate cancer cell invasion via P2Y receptors. However, the purinergic receptor subtype(s) involved in this process remains unclear. Here we aimed to determine whether P2Y2, one subtype of P2Y receptors, was involved in the invasion and metastasis of prostate cancer cells, and elucidated the underlying mechanism. METHODS: RNAi was introduced to silence the expression of P2Y2. In vitro invasion and migration assays and in vivo experiments were carried out to examine the role of P2Y2 receptor in cell invasion and metastasis. cDNA microarray was performed to identify the differentially expressed genes downstream of ATP treatment. RESULTS: P2Y2 was significantly expressed in the prostate cancer cells. Knockdown of P2Y2 receptor suppressed cell invasion and metastasis in vitro and in vivo. Further experiments identified that ATP could promote IL-8 and Snail expression and inhibit E-cadherin and Claudin-1 expression. Knockdown of P2Y2 receptor affected the expression of these EMT/invasion-related genes in vitro and in vivo. CONCLUSION: P2Y2 receptor promotes cell invasion and metastasis in prostate cancer cells via some EMT/invasion-related genes. Thereby, P2Y2 receptor could be a potential therapeutic target for the treatment of prostate cancer. Nature Publishing Group 2013-09-17 2013-08-22 /pmc/articles/PMC3776994/ /pubmed/23969730 http://dx.doi.org/10.1038/bjc.2013.484 Text en Copyright © 2013 Cancer Research UK http://creativecommons.org/licenses/by-nc-sa/3.0/ From twelve months after its original publication, this work is licensed under the Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
spellingShingle Molecular Diagnostics
Li, W-H
Qiu, Y
Zhang, H-Q
Liu, Y
You, J-F
Tian, X-X
Fang, W-G
P2Y2 receptor promotes cell invasion and metastasis in prostate cancer cells
title P2Y2 receptor promotes cell invasion and metastasis in prostate cancer cells
title_full P2Y2 receptor promotes cell invasion and metastasis in prostate cancer cells
title_fullStr P2Y2 receptor promotes cell invasion and metastasis in prostate cancer cells
title_full_unstemmed P2Y2 receptor promotes cell invasion and metastasis in prostate cancer cells
title_short P2Y2 receptor promotes cell invasion and metastasis in prostate cancer cells
title_sort p2y2 receptor promotes cell invasion and metastasis in prostate cancer cells
topic Molecular Diagnostics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3776994/
https://www.ncbi.nlm.nih.gov/pubmed/23969730
http://dx.doi.org/10.1038/bjc.2013.484
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