Improvement of innate immune responses and defense activity in tiger shrimp (Penaeus monodon Fab.) by intramuscular administration of the outer membrane protein Vibrio alginolyticus

The Outer Membrane Protein (OMP) of Vibrio alginolyticus cell wall was administered intramuscularly (IM) to the tiger shrimp (Penaeus monodon Fab.) at 10, 20, 30 μg/kg bw. After 14 days infection, the tiger shrimps were challenged with 10(7) bacterial density of Vibrio harveyi for 24 hours. The tota...

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Autores principales: Maftuch, Prasetio, E, Sudianto, A, Rozik, M, Nurdiyani, R, Sanusi, E, Nursyam, H, Fariedah, F, Marsoedi, Murachman
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2013
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3777020/
https://www.ncbi.nlm.nih.gov/pubmed/24058892
http://dx.doi.org/10.1186/2193-1801-2-432
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author Maftuch
Prasetio, E
Sudianto, A
Rozik, M
Nurdiyani, R
Sanusi, E
Nursyam, H
Fariedah, F
Marsoedi
Murachman
author_facet Maftuch
Prasetio, E
Sudianto, A
Rozik, M
Nurdiyani, R
Sanusi, E
Nursyam, H
Fariedah, F
Marsoedi
Murachman
author_sort Maftuch
collection PubMed
description The Outer Membrane Protein (OMP) of Vibrio alginolyticus cell wall was administered intramuscularly (IM) to the tiger shrimp (Penaeus monodon Fab.) at 10, 20, 30 μg/kg bw. After 14 days infection, the tiger shrimps were challenged with 10(7) bacterial density of Vibrio harveyi for 24 hours. The total haemocyte count (THC), differential haemocyte count (DHC) and amount of total protein plasma (TPP), superoxide dismutase and protease enzyme activity were monitored. The results showed that intramuscular administration of OMP enhanced an immunomodulatory effect and protection against V. harveyi. The beneficial effect of OMP on the tiger shrimp is dose-dependent and OMP-20 μg/kg bw is an optimal dose after two times of boosters for 14 days against V. harveyi infection.
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spelling pubmed-37770202013-09-20 Improvement of innate immune responses and defense activity in tiger shrimp (Penaeus monodon Fab.) by intramuscular administration of the outer membrane protein Vibrio alginolyticus Maftuch Prasetio, E Sudianto, A Rozik, M Nurdiyani, R Sanusi, E Nursyam, H Fariedah, F Marsoedi Murachman Springerplus Research The Outer Membrane Protein (OMP) of Vibrio alginolyticus cell wall was administered intramuscularly (IM) to the tiger shrimp (Penaeus monodon Fab.) at 10, 20, 30 μg/kg bw. After 14 days infection, the tiger shrimps were challenged with 10(7) bacterial density of Vibrio harveyi for 24 hours. The total haemocyte count (THC), differential haemocyte count (DHC) and amount of total protein plasma (TPP), superoxide dismutase and protease enzyme activity were monitored. The results showed that intramuscular administration of OMP enhanced an immunomodulatory effect and protection against V. harveyi. The beneficial effect of OMP on the tiger shrimp is dose-dependent and OMP-20 μg/kg bw is an optimal dose after two times of boosters for 14 days against V. harveyi infection. Springer International Publishing 2013-09-03 /pmc/articles/PMC3777020/ /pubmed/24058892 http://dx.doi.org/10.1186/2193-1801-2-432 Text en © Maftuch et al.; licensee Springer. 2013 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Maftuch
Prasetio, E
Sudianto, A
Rozik, M
Nurdiyani, R
Sanusi, E
Nursyam, H
Fariedah, F
Marsoedi
Murachman
Improvement of innate immune responses and defense activity in tiger shrimp (Penaeus monodon Fab.) by intramuscular administration of the outer membrane protein Vibrio alginolyticus
title Improvement of innate immune responses and defense activity in tiger shrimp (Penaeus monodon Fab.) by intramuscular administration of the outer membrane protein Vibrio alginolyticus
title_full Improvement of innate immune responses and defense activity in tiger shrimp (Penaeus monodon Fab.) by intramuscular administration of the outer membrane protein Vibrio alginolyticus
title_fullStr Improvement of innate immune responses and defense activity in tiger shrimp (Penaeus monodon Fab.) by intramuscular administration of the outer membrane protein Vibrio alginolyticus
title_full_unstemmed Improvement of innate immune responses and defense activity in tiger shrimp (Penaeus monodon Fab.) by intramuscular administration of the outer membrane protein Vibrio alginolyticus
title_short Improvement of innate immune responses and defense activity in tiger shrimp (Penaeus monodon Fab.) by intramuscular administration of the outer membrane protein Vibrio alginolyticus
title_sort improvement of innate immune responses and defense activity in tiger shrimp (penaeus monodon fab.) by intramuscular administration of the outer membrane protein vibrio alginolyticus
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3777020/
https://www.ncbi.nlm.nih.gov/pubmed/24058892
http://dx.doi.org/10.1186/2193-1801-2-432
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