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Chromatin in a marine picoeukaryote is a disordered assemblage of nucleosomes

Chromatin organization is central to many conserved biological processes, but it is generally unknown how the underlying nucleosomes are arranged in situ. Here, we have used electron cryotomography to study chromatin in the picoplankton Ostreococcus tauri, the smallest known free-living eukaryote. B...

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Detalles Bibliográficos
Autores principales: Gan, Lu, Ladinsky, Mark S., Jensen, Grant J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3777167/
https://www.ncbi.nlm.nih.gov/pubmed/23818178
http://dx.doi.org/10.1007/s00412-013-0423-z
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author Gan, Lu
Ladinsky, Mark S.
Jensen, Grant J.
author_facet Gan, Lu
Ladinsky, Mark S.
Jensen, Grant J.
author_sort Gan, Lu
collection PubMed
description Chromatin organization is central to many conserved biological processes, but it is generally unknown how the underlying nucleosomes are arranged in situ. Here, we have used electron cryotomography to study chromatin in the picoplankton Ostreococcus tauri, the smallest known free-living eukaryote. By visualizing the nucleosome densities directly, we find that O. tauri chromosomes do not arrange into discrete, compact bodies or any other higher level of order. In contrast to the textbook 30-nm fiber model, O. tauri chromatin resembles a disordered assemblage of nucleosomes akin to the polymer melt model. This disorganized nucleosome arrangement has important implications for potentially conserved functions in tiny eukaryotes such as the clustering of nonhomologous chromosomes at the kinetochore during mitosis and the independent regulation of closely positioned adjacent genes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00412-013-0423-z) contains supplementary material, which is available to authorized users.
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spelling pubmed-37771672013-09-25 Chromatin in a marine picoeukaryote is a disordered assemblage of nucleosomes Gan, Lu Ladinsky, Mark S. Jensen, Grant J. Chromosoma Research Article Chromatin organization is central to many conserved biological processes, but it is generally unknown how the underlying nucleosomes are arranged in situ. Here, we have used electron cryotomography to study chromatin in the picoplankton Ostreococcus tauri, the smallest known free-living eukaryote. By visualizing the nucleosome densities directly, we find that O. tauri chromosomes do not arrange into discrete, compact bodies or any other higher level of order. In contrast to the textbook 30-nm fiber model, O. tauri chromatin resembles a disordered assemblage of nucleosomes akin to the polymer melt model. This disorganized nucleosome arrangement has important implications for potentially conserved functions in tiny eukaryotes such as the clustering of nonhomologous chromosomes at the kinetochore during mitosis and the independent regulation of closely positioned adjacent genes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00412-013-0423-z) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2013-07-03 2013 /pmc/articles/PMC3777167/ /pubmed/23818178 http://dx.doi.org/10.1007/s00412-013-0423-z Text en © The Author(s) 2013 https://creativecommons.org/licenses/by-nc/2.0/ Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Research Article
Gan, Lu
Ladinsky, Mark S.
Jensen, Grant J.
Chromatin in a marine picoeukaryote is a disordered assemblage of nucleosomes
title Chromatin in a marine picoeukaryote is a disordered assemblage of nucleosomes
title_full Chromatin in a marine picoeukaryote is a disordered assemblage of nucleosomes
title_fullStr Chromatin in a marine picoeukaryote is a disordered assemblage of nucleosomes
title_full_unstemmed Chromatin in a marine picoeukaryote is a disordered assemblage of nucleosomes
title_short Chromatin in a marine picoeukaryote is a disordered assemblage of nucleosomes
title_sort chromatin in a marine picoeukaryote is a disordered assemblage of nucleosomes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3777167/
https://www.ncbi.nlm.nih.gov/pubmed/23818178
http://dx.doi.org/10.1007/s00412-013-0423-z
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