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Long-term molecular changes in WHO grade II astrocytomas following radiotherapy
Monitoring the long-term radiotherapy-associated molecular changes in low-grade gliomas (LGGs) facilitates the understanding of LGG response to radiotherapy. In this study, we used immunohistochemistry to analyze the expression of Ki-67, tumor protein P53 (TP53), P21, and P27 in 8 paired WHO grade I...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Sun Yat-sen University Cancer Center
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3777483/ https://www.ncbi.nlm.nih.gov/pubmed/22313596 http://dx.doi.org/10.5732/cjc.011.10149 |
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author | Yue, Wei-Ying Sai, Ke Wu, Qiu-Liang Xia, Yun-Fei Yu, Su-Huan Chen, Zhong-Ping |
author_facet | Yue, Wei-Ying Sai, Ke Wu, Qiu-Liang Xia, Yun-Fei Yu, Su-Huan Chen, Zhong-Ping |
author_sort | Yue, Wei-Ying |
collection | PubMed |
description | Monitoring the long-term radiotherapy-associated molecular changes in low-grade gliomas (LGGs) facilitates the understanding of LGG response to radiotherapy. In this study, we used immunohistochemistry to analyze the expression of Ki-67, tumor protein P53 (TP53), P21, and P27 in 8 paired WHO grade II astrocytoma samples. The interval between radiotherapy (RT) and the second surgery was more than 3 months in all cases. The average Ki-67 labeling index (LI) was 5.3% in pre-RT samples and 11.54% in post-RT samples. Ki-67 LI was higher in the primary tumors that underwent malignant transformation observed at the second surgery after radiation. Post-RT Ki-67 LI decreased in 2 cases with an interval of less than 12 months between RT and the second surgery. TP53 expression was found in 3 out of 4 pre-RT samples with malignant transformation and in 1 out of 4 pre-RT samples without malignant transformation. Post-RT TP53 increased in 2 cases in which increased expression of P21 or P27 was also observed. Our study suggests that radiotherapy can inhibit WHO grade II astrocytoma proliferation as reflected by Ki-67 LI, but the effect attenuates with time. In addition, there is a tendency of malignant transformation for WHO grade II astrocytomas with a high Ki-67 level or TP53 expression in initial samples. |
format | Online Article Text |
id | pubmed-3777483 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Sun Yat-sen University Cancer Center |
record_format | MEDLINE/PubMed |
spelling | pubmed-37774832013-12-11 Long-term molecular changes in WHO grade II astrocytomas following radiotherapy Yue, Wei-Ying Sai, Ke Wu, Qiu-Liang Xia, Yun-Fei Yu, Su-Huan Chen, Zhong-Ping Chin J Cancer Original Article Monitoring the long-term radiotherapy-associated molecular changes in low-grade gliomas (LGGs) facilitates the understanding of LGG response to radiotherapy. In this study, we used immunohistochemistry to analyze the expression of Ki-67, tumor protein P53 (TP53), P21, and P27 in 8 paired WHO grade II astrocytoma samples. The interval between radiotherapy (RT) and the second surgery was more than 3 months in all cases. The average Ki-67 labeling index (LI) was 5.3% in pre-RT samples and 11.54% in post-RT samples. Ki-67 LI was higher in the primary tumors that underwent malignant transformation observed at the second surgery after radiation. Post-RT Ki-67 LI decreased in 2 cases with an interval of less than 12 months between RT and the second surgery. TP53 expression was found in 3 out of 4 pre-RT samples with malignant transformation and in 1 out of 4 pre-RT samples without malignant transformation. Post-RT TP53 increased in 2 cases in which increased expression of P21 or P27 was also observed. Our study suggests that radiotherapy can inhibit WHO grade II astrocytoma proliferation as reflected by Ki-67 LI, but the effect attenuates with time. In addition, there is a tendency of malignant transformation for WHO grade II astrocytomas with a high Ki-67 level or TP53 expression in initial samples. Sun Yat-sen University Cancer Center 2012-03 /pmc/articles/PMC3777483/ /pubmed/22313596 http://dx.doi.org/10.5732/cjc.011.10149 Text en Chinese Journal of Cancer http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License, which allows readers to alter, transform, or build upon the article and then distribute the resulting work under the same or similar license to this one. The work must be attributed back to the original author and commercial use is not permitted without specific permission. |
spellingShingle | Original Article Yue, Wei-Ying Sai, Ke Wu, Qiu-Liang Xia, Yun-Fei Yu, Su-Huan Chen, Zhong-Ping Long-term molecular changes in WHO grade II astrocytomas following radiotherapy |
title | Long-term molecular changes in WHO grade II astrocytomas following radiotherapy |
title_full | Long-term molecular changes in WHO grade II astrocytomas following radiotherapy |
title_fullStr | Long-term molecular changes in WHO grade II astrocytomas following radiotherapy |
title_full_unstemmed | Long-term molecular changes in WHO grade II astrocytomas following radiotherapy |
title_short | Long-term molecular changes in WHO grade II astrocytomas following radiotherapy |
title_sort | long-term molecular changes in who grade ii astrocytomas following radiotherapy |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3777483/ https://www.ncbi.nlm.nih.gov/pubmed/22313596 http://dx.doi.org/10.5732/cjc.011.10149 |
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