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Mutually exclusive signaling signatures define the hepatic and pancreatic progenitor cell lineage divergence
Understanding how distinct cell types arise from multipotent progenitor cells is a major quest in stem cell biology. The liver and pancreas share many aspects of their early development and possibly originate from a common progenitor. However, how liver and pancreas cells diverge from a common endod...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3778245/ https://www.ncbi.nlm.nih.gov/pubmed/24013505 http://dx.doi.org/10.1101/gad.220244.113 |
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author | Rodríguez-Seguel, Elisa Mah, Nancy Naumann, Heike Pongrac, Igor M. Cerdá-Esteban, Nuria Fontaine, Jean-Fred Wang, Yongbo Chen, Wei Andrade-Navarro, Miguel A. Spagnoli, Francesca M. |
author_facet | Rodríguez-Seguel, Elisa Mah, Nancy Naumann, Heike Pongrac, Igor M. Cerdá-Esteban, Nuria Fontaine, Jean-Fred Wang, Yongbo Chen, Wei Andrade-Navarro, Miguel A. Spagnoli, Francesca M. |
author_sort | Rodríguez-Seguel, Elisa |
collection | PubMed |
description | Understanding how distinct cell types arise from multipotent progenitor cells is a major quest in stem cell biology. The liver and pancreas share many aspects of their early development and possibly originate from a common progenitor. However, how liver and pancreas cells diverge from a common endoderm progenitor population and adopt specific fates remains elusive. Using RNA sequencing (RNA-seq), we defined the molecular identity of liver and pancreas progenitors that were isolated from the mouse embryo at two time points, spanning the period when the lineage decision is made. The integration of temporal and spatial gene expression profiles unveiled mutually exclusive signaling signatures in hepatic and pancreatic progenitors. Importantly, we identified the noncanonical Wnt pathway as a potential developmental regulator of this fate decision and capable of inducing the pancreas program in endoderm and liver cells. Our study offers an unprecedented view of gene expression programs in liver and pancreas progenitors and forms the basis for formulating lineage-reprogramming strategies to convert adult hepatic cells into pancreatic cells. |
format | Online Article Text |
id | pubmed-3778245 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-37782452014-03-01 Mutually exclusive signaling signatures define the hepatic and pancreatic progenitor cell lineage divergence Rodríguez-Seguel, Elisa Mah, Nancy Naumann, Heike Pongrac, Igor M. Cerdá-Esteban, Nuria Fontaine, Jean-Fred Wang, Yongbo Chen, Wei Andrade-Navarro, Miguel A. Spagnoli, Francesca M. Genes Dev Resource/Methodology Understanding how distinct cell types arise from multipotent progenitor cells is a major quest in stem cell biology. The liver and pancreas share many aspects of their early development and possibly originate from a common progenitor. However, how liver and pancreas cells diverge from a common endoderm progenitor population and adopt specific fates remains elusive. Using RNA sequencing (RNA-seq), we defined the molecular identity of liver and pancreas progenitors that were isolated from the mouse embryo at two time points, spanning the period when the lineage decision is made. The integration of temporal and spatial gene expression profiles unveiled mutually exclusive signaling signatures in hepatic and pancreatic progenitors. Importantly, we identified the noncanonical Wnt pathway as a potential developmental regulator of this fate decision and capable of inducing the pancreas program in endoderm and liver cells. Our study offers an unprecedented view of gene expression programs in liver and pancreas progenitors and forms the basis for formulating lineage-reprogramming strategies to convert adult hepatic cells into pancreatic cells. Cold Spring Harbor Laboratory Press 2013-09-01 /pmc/articles/PMC3778245/ /pubmed/24013505 http://dx.doi.org/10.1101/gad.220244.113 Text en © 2013, Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/3.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/. |
spellingShingle | Resource/Methodology Rodríguez-Seguel, Elisa Mah, Nancy Naumann, Heike Pongrac, Igor M. Cerdá-Esteban, Nuria Fontaine, Jean-Fred Wang, Yongbo Chen, Wei Andrade-Navarro, Miguel A. Spagnoli, Francesca M. Mutually exclusive signaling signatures define the hepatic and pancreatic progenitor cell lineage divergence |
title | Mutually exclusive signaling signatures define the hepatic and pancreatic progenitor cell lineage divergence |
title_full | Mutually exclusive signaling signatures define the hepatic and pancreatic progenitor cell lineage divergence |
title_fullStr | Mutually exclusive signaling signatures define the hepatic and pancreatic progenitor cell lineage divergence |
title_full_unstemmed | Mutually exclusive signaling signatures define the hepatic and pancreatic progenitor cell lineage divergence |
title_short | Mutually exclusive signaling signatures define the hepatic and pancreatic progenitor cell lineage divergence |
title_sort | mutually exclusive signaling signatures define the hepatic and pancreatic progenitor cell lineage divergence |
topic | Resource/Methodology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3778245/ https://www.ncbi.nlm.nih.gov/pubmed/24013505 http://dx.doi.org/10.1101/gad.220244.113 |
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