In vitro antioxidant and H(+), K(+)-ATPase inhibition activities of Acalypha wilkesiana foliage extract

AIMS: The aim of this study was to evaluate the antioxidant activty and anti-acid property of Acalypha wilkesiana foliage extract. MATERIALS AND METHODS: Hot and cold aqueous extracts were prepared from healthy leaves of A. wilkesiana. Free radical scavenging activity and H(+), K(+)-ATPase inhibition activities of aqueous foliage extracts was screened by in vitro models. STATISTICAL ANALYSIS USED: All experiments were performed in triplicate and results are expressed as mean ± SEM. RESULTS: A. wilkesiana hot aqueous extract (AWHE) showed significant antioxidants and free radical scavenging activity. Further, AWHE has shown a potent H(+), K(+)-ATPase inhibitory activity (IC(50): 51.5 ± 0.28 μg/ml) when compare to standard proton pump inhibitor omeprazole (56.2 ± 0.64 μg/ml); however, latter activity is equal to A. wilkesiana cold aqueous extract (AWCE). Quantitative analysis of AWHE has revealed more content of phenols and flavonoids; this is found to be the reason for good antioxidant activity over AWCE. Molecular docking was carried out against H(+), K(+)-ATPase enzyme crystal structure to validate the anti-acid activity of A. wilkesiana major phytochemicals. CONCLUSIONS: The present study indicates that the constituents of AWHE and AWCE have good antacid and free radical scavenging activity.