Cargando…

Cryomicroscopy of radiation sensitive specimens on unmodified graphene sheets: Reduction of electron-optical effects of charging()

Images of radiation-sensitive specimens obtained by electron microscopy suffer a reduction in quality beyond that expected from radiation damage alone due to electron beam-induced charging or movement of the specimen. For biological specimens, charging and movement are most severe when they are susp...

Descripción completa

Detalles Bibliográficos
Autores principales: Sader, Kasim, Stopps, Martyn, Calder, Lesley J., Rosenthal, Peter B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academic Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3778975/
https://www.ncbi.nlm.nih.gov/pubmed/23664842
http://dx.doi.org/10.1016/j.jsb.2013.04.014
_version_ 1782285187678208000
author Sader, Kasim
Stopps, Martyn
Calder, Lesley J.
Rosenthal, Peter B.
author_facet Sader, Kasim
Stopps, Martyn
Calder, Lesley J.
Rosenthal, Peter B.
author_sort Sader, Kasim
collection PubMed
description Images of radiation-sensitive specimens obtained by electron microscopy suffer a reduction in quality beyond that expected from radiation damage alone due to electron beam-induced charging or movement of the specimen. For biological specimens, charging and movement are most severe when they are suspended in an insulating layer of vitreous ice, which is otherwise optimal for preserving hydrated specimens in a near native state. We image biological specimens, including a single particle protein complex and a lipid-enveloped virus in thin, vitreous ice films over suspended sheets of unmodified graphene. We show that in such preparations, the charging of ice, as assessed by electron-optical perturbation of the imaging beam, is eliminated. We also use the same specimen supports to record high resolution images at liquid nitrogen temperature of monolayer paraffin crystals grown over graphene.
format Online
Article
Text
id pubmed-3778975
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Academic Press
record_format MEDLINE/PubMed
spelling pubmed-37789752013-09-23 Cryomicroscopy of radiation sensitive specimens on unmodified graphene sheets: Reduction of electron-optical effects of charging() Sader, Kasim Stopps, Martyn Calder, Lesley J. Rosenthal, Peter B. J Struct Biol Technical Note Images of radiation-sensitive specimens obtained by electron microscopy suffer a reduction in quality beyond that expected from radiation damage alone due to electron beam-induced charging or movement of the specimen. For biological specimens, charging and movement are most severe when they are suspended in an insulating layer of vitreous ice, which is otherwise optimal for preserving hydrated specimens in a near native state. We image biological specimens, including a single particle protein complex and a lipid-enveloped virus in thin, vitreous ice films over suspended sheets of unmodified graphene. We show that in such preparations, the charging of ice, as assessed by electron-optical perturbation of the imaging beam, is eliminated. We also use the same specimen supports to record high resolution images at liquid nitrogen temperature of monolayer paraffin crystals grown over graphene. Academic Press 2013-09 /pmc/articles/PMC3778975/ /pubmed/23664842 http://dx.doi.org/10.1016/j.jsb.2013.04.014 Text en © 2013 The Authors https://creativecommons.org/licenses/by-nc-nd/3.0/ Open Access under CC BY-NC-ND 3.0 (https://creativecommons.org/licenses/by-nc-nd/3.0/) license
spellingShingle Technical Note
Sader, Kasim
Stopps, Martyn
Calder, Lesley J.
Rosenthal, Peter B.
Cryomicroscopy of radiation sensitive specimens on unmodified graphene sheets: Reduction of electron-optical effects of charging()
title Cryomicroscopy of radiation sensitive specimens on unmodified graphene sheets: Reduction of electron-optical effects of charging()
title_full Cryomicroscopy of radiation sensitive specimens on unmodified graphene sheets: Reduction of electron-optical effects of charging()
title_fullStr Cryomicroscopy of radiation sensitive specimens on unmodified graphene sheets: Reduction of electron-optical effects of charging()
title_full_unstemmed Cryomicroscopy of radiation sensitive specimens on unmodified graphene sheets: Reduction of electron-optical effects of charging()
title_short Cryomicroscopy of radiation sensitive specimens on unmodified graphene sheets: Reduction of electron-optical effects of charging()
title_sort cryomicroscopy of radiation sensitive specimens on unmodified graphene sheets: reduction of electron-optical effects of charging()
topic Technical Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3778975/
https://www.ncbi.nlm.nih.gov/pubmed/23664842
http://dx.doi.org/10.1016/j.jsb.2013.04.014
work_keys_str_mv AT saderkasim cryomicroscopyofradiationsensitivespecimensonunmodifiedgraphenesheetsreductionofelectronopticaleffectsofcharging
AT stoppsmartyn cryomicroscopyofradiationsensitivespecimensonunmodifiedgraphenesheetsreductionofelectronopticaleffectsofcharging
AT calderlesleyj cryomicroscopyofradiationsensitivespecimensonunmodifiedgraphenesheetsreductionofelectronopticaleffectsofcharging
AT rosenthalpeterb cryomicroscopyofradiationsensitivespecimensonunmodifiedgraphenesheetsreductionofelectronopticaleffectsofcharging