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Novel 3D Co-Culture Model for Epithelial-Stromal Cells Interaction in Prostate Cancer

Paracrine function is a major mechanism of cell-cell communication within tissue microenvironment in normal development and disease. In vitro cell culture models simulating tissue or tumor microenvironment are necessary tools to delineate epithelial-stromal interactions including paracrine function,...

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Autores principales: Fang, Xiaolan, Sittadjody, Sivanandane, Gyabaah, Kenneth, Opara, Emmanuel C., Balaji, Kethandapatti C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3779160/
https://www.ncbi.nlm.nih.gov/pubmed/24073251
http://dx.doi.org/10.1371/journal.pone.0075187
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author Fang, Xiaolan
Sittadjody, Sivanandane
Gyabaah, Kenneth
Opara, Emmanuel C.
Balaji, Kethandapatti C.
author_facet Fang, Xiaolan
Sittadjody, Sivanandane
Gyabaah, Kenneth
Opara, Emmanuel C.
Balaji, Kethandapatti C.
author_sort Fang, Xiaolan
collection PubMed
description Paracrine function is a major mechanism of cell-cell communication within tissue microenvironment in normal development and disease. In vitro cell culture models simulating tissue or tumor microenvironment are necessary tools to delineate epithelial-stromal interactions including paracrine function, yet an ideal three-dimensional (3D) tumor model specifically studying paracrine function is currently lacking. In order to fill this void we developed a novel 3D co-culture model in double-layered alginate hydrogel microspheres, incorporating prostate cancer epithelial and stromal cells in separate compartments of the microspheres. The cells remained confined and viable within their respective spheres for over 30 days. As a proof of principle regarding paracrine function of the model, we measured shedded component of E-cadherin (sE-cad) in the conditioned media, a major membrane bound cell adhesive molecule that is highly dysregulated in cancers including prostate cancer. In addition to demonstrating that sE-cad can be reliably quantified in the conditioned media, the time course experiments also demonstrated that the amount of sE-cad is influenced by epithelial-stromal interaction. In conclusion, the study establishes a novel 3D in vitro co-culture model that can be used to study cell-cell paracrine interaction.
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spelling pubmed-37791602013-09-26 Novel 3D Co-Culture Model for Epithelial-Stromal Cells Interaction in Prostate Cancer Fang, Xiaolan Sittadjody, Sivanandane Gyabaah, Kenneth Opara, Emmanuel C. Balaji, Kethandapatti C. PLoS One Research Article Paracrine function is a major mechanism of cell-cell communication within tissue microenvironment in normal development and disease. In vitro cell culture models simulating tissue or tumor microenvironment are necessary tools to delineate epithelial-stromal interactions including paracrine function, yet an ideal three-dimensional (3D) tumor model specifically studying paracrine function is currently lacking. In order to fill this void we developed a novel 3D co-culture model in double-layered alginate hydrogel microspheres, incorporating prostate cancer epithelial and stromal cells in separate compartments of the microspheres. The cells remained confined and viable within their respective spheres for over 30 days. As a proof of principle regarding paracrine function of the model, we measured shedded component of E-cadherin (sE-cad) in the conditioned media, a major membrane bound cell adhesive molecule that is highly dysregulated in cancers including prostate cancer. In addition to demonstrating that sE-cad can be reliably quantified in the conditioned media, the time course experiments also demonstrated that the amount of sE-cad is influenced by epithelial-stromal interaction. In conclusion, the study establishes a novel 3D in vitro co-culture model that can be used to study cell-cell paracrine interaction. Public Library of Science 2013-09-20 /pmc/articles/PMC3779160/ /pubmed/24073251 http://dx.doi.org/10.1371/journal.pone.0075187 Text en © 2013 Fang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Fang, Xiaolan
Sittadjody, Sivanandane
Gyabaah, Kenneth
Opara, Emmanuel C.
Balaji, Kethandapatti C.
Novel 3D Co-Culture Model for Epithelial-Stromal Cells Interaction in Prostate Cancer
title Novel 3D Co-Culture Model for Epithelial-Stromal Cells Interaction in Prostate Cancer
title_full Novel 3D Co-Culture Model for Epithelial-Stromal Cells Interaction in Prostate Cancer
title_fullStr Novel 3D Co-Culture Model for Epithelial-Stromal Cells Interaction in Prostate Cancer
title_full_unstemmed Novel 3D Co-Culture Model for Epithelial-Stromal Cells Interaction in Prostate Cancer
title_short Novel 3D Co-Culture Model for Epithelial-Stromal Cells Interaction in Prostate Cancer
title_sort novel 3d co-culture model for epithelial-stromal cells interaction in prostate cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3779160/
https://www.ncbi.nlm.nih.gov/pubmed/24073251
http://dx.doi.org/10.1371/journal.pone.0075187
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