High-Throughput RNA FISH Analysis by Imaging Flow Cytometry Reveals That Pioneer Factor Foxa1 Reduces Transcriptional Stochasticity

Genes are regulated at the single-cell level. Here, we performed RNA FISH of thousands of cells by flow cytometry (flow-RNA FISH) to gain insight into transcriptional variability between individual cells. These experiments utilized the murine adenocarcinoma 3134 cell line with 200 copies of the MMTV...

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Detalles Bibliográficos
Autores principales: Lalmansingh, Avin S., Arora, Kamalpreet, DeMarco, Richard A., Hager, Gordon L., Nagaich, Akhilesh K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3779185/
https://www.ncbi.nlm.nih.gov/pubmed/24073287
http://dx.doi.org/10.1371/journal.pone.0076043

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