Selection of Single Domain Antibodies from Immune Libraries Displayed on the Surface of E. coli Cells with Two β-Domains of Opposite Topologies

Screening of antibody (Ab) libraries by direct display on the surface of E. coli cells is hampered by the presence of the outer membrane (OM). In this work we demonstrate that the native β-domains of EhaA autotransporter and intimin, two proteins from enterohemorrhagic E. coli O157:H7 (EHEC) with op...

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Detalles Bibliográficos
Autores principales: Salema, Valencio, Marín, Elvira, Martínez-Arteaga, Rocio, Ruano-Gallego, David, Fraile, Sofía, Margolles, Yago, Teira, Xema, Gutierrez, Carlos, Bodelón, Gustavo, Fernández, Luis Ángel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3781032/
https://www.ncbi.nlm.nih.gov/pubmed/24086454
http://dx.doi.org/10.1371/journal.pone.0075126

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