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Development of semi-defined rice straw-based medium for butanol production and its kinetic study

Rice straw is one of the potential economic feedstock for biobutanol production through ABE fermentation. However, the rice straw hydrolysate-based fermentation medium needs to be supported with nutritional elements. In this study, an attempt is made to optimize the rice straw hydrolysate-based ferm...

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Autores principales: Ranjan, Amrita, Mayank, Rahul, Moholkar, Vijayanand S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3781261/
https://www.ncbi.nlm.nih.gov/pubmed/28324335
http://dx.doi.org/10.1007/s13205-013-0120-x
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author Ranjan, Amrita
Mayank, Rahul
Moholkar, Vijayanand S.
author_facet Ranjan, Amrita
Mayank, Rahul
Moholkar, Vijayanand S.
author_sort Ranjan, Amrita
collection PubMed
description Rice straw is one of the potential economic feedstock for biobutanol production through ABE fermentation. However, the rice straw hydrolysate-based fermentation medium needs to be supported with nutritional elements. In this study, an attempt is made to optimize the rice straw hydrolysate-based fermentation medium employing Clostridium acetobutylicum MTCC 481 using Taguchi design of experiments (DOE) statistical model. Initially, a set of 12 nutrient components viz. MgNO(3)·6H(2)O, FeNO(3), NH(4)NO(3), yeast extract, PABA, biotin, PABA + biotin mixture, CaCl(2), KCl, NaCl, MgSO(4) and CH(3)COONa were screened through classical (one-variable-at-a-time) method. Based on the results, four components (PABA, yeast extract, MgSO(4) and CH(3)COONa) were found to have significant impact, and were further subjected to statistical optimization through Taguchi DOE method. These experiments revealed that RSH supported with 3 g L(−1) of yeast extract and 4 mg L(−1) PABA to RSH was the most optimum fermentation medium. Experiments using 2 L bioreactor with this optimum fermentation medium showed nearly complete utilization of soluble sugars with the production of 8.7 g L(−1) of total solvents and 6 g L(−1) of butanol. The experimental data were fitted to kinetic models reported in the literature to determine the kinetic parameters of the fermentation process. An interesting result was revealed from this analysis that the under optimized fermentation medium, the kinetic parameters for both shake flask and bioreactor level were similar. This essentially means that effect of scale of operation is rendered insignificant when fermentation medium is under optimum conditions. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s13205-013-0120-x) contains supplementary material, which is available to authorized users.
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spelling pubmed-37812612013-09-25 Development of semi-defined rice straw-based medium for butanol production and its kinetic study Ranjan, Amrita Mayank, Rahul Moholkar, Vijayanand S. 3 Biotech Original Article Rice straw is one of the potential economic feedstock for biobutanol production through ABE fermentation. However, the rice straw hydrolysate-based fermentation medium needs to be supported with nutritional elements. In this study, an attempt is made to optimize the rice straw hydrolysate-based fermentation medium employing Clostridium acetobutylicum MTCC 481 using Taguchi design of experiments (DOE) statistical model. Initially, a set of 12 nutrient components viz. MgNO(3)·6H(2)O, FeNO(3), NH(4)NO(3), yeast extract, PABA, biotin, PABA + biotin mixture, CaCl(2), KCl, NaCl, MgSO(4) and CH(3)COONa were screened through classical (one-variable-at-a-time) method. Based on the results, four components (PABA, yeast extract, MgSO(4) and CH(3)COONa) were found to have significant impact, and were further subjected to statistical optimization through Taguchi DOE method. These experiments revealed that RSH supported with 3 g L(−1) of yeast extract and 4 mg L(−1) PABA to RSH was the most optimum fermentation medium. Experiments using 2 L bioreactor with this optimum fermentation medium showed nearly complete utilization of soluble sugars with the production of 8.7 g L(−1) of total solvents and 6 g L(−1) of butanol. The experimental data were fitted to kinetic models reported in the literature to determine the kinetic parameters of the fermentation process. An interesting result was revealed from this analysis that the under optimized fermentation medium, the kinetic parameters for both shake flask and bioreactor level were similar. This essentially means that effect of scale of operation is rendered insignificant when fermentation medium is under optimum conditions. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s13205-013-0120-x) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2013-03-09 2013-10 /pmc/articles/PMC3781261/ /pubmed/28324335 http://dx.doi.org/10.1007/s13205-013-0120-x Text en © The Author(s) 2013 https://creativecommons.org/licenses/by/2.0/ This article is published under license to BioMed Central Ltd. Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Original Article
Ranjan, Amrita
Mayank, Rahul
Moholkar, Vijayanand S.
Development of semi-defined rice straw-based medium for butanol production and its kinetic study
title Development of semi-defined rice straw-based medium for butanol production and its kinetic study
title_full Development of semi-defined rice straw-based medium for butanol production and its kinetic study
title_fullStr Development of semi-defined rice straw-based medium for butanol production and its kinetic study
title_full_unstemmed Development of semi-defined rice straw-based medium for butanol production and its kinetic study
title_short Development of semi-defined rice straw-based medium for butanol production and its kinetic study
title_sort development of semi-defined rice straw-based medium for butanol production and its kinetic study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3781261/
https://www.ncbi.nlm.nih.gov/pubmed/28324335
http://dx.doi.org/10.1007/s13205-013-0120-x
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