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Radixin inhibition decreases adult neural progenitor cell migration and proliferation in vitro and in vivo
Neuronal progenitors capable of long distance migration are produced throughout life in the subventricular zone (SVZ). Migration from the SVZ is carried out along a well-defined pathway called the rostral migratory stream (RMS). Our recent finding of the specific expression of the cytoskeleton linke...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2013
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3781578/ https://www.ncbi.nlm.nih.gov/pubmed/24065889 http://dx.doi.org/10.3389/fncel.2013.00161 |
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author | Persson, Åsa Lindberg, Olle R. Kuhn, Hans G. |
author_facet | Persson, Åsa Lindberg, Olle R. Kuhn, Hans G. |
author_sort | Persson, Åsa |
collection | PubMed |
description | Neuronal progenitors capable of long distance migration are produced throughout life in the subventricular zone (SVZ). Migration from the SVZ is carried out along a well-defined pathway called the rostral migratory stream (RMS). Our recent finding of the specific expression of the cytoskeleton linker protein radixin in neuroblasts suggests a functional role for radixin in RMS migration. The ezrin-radixin-moesin (ERM) family of proteins is capable of regulating migration through interaction with the actin cytoskeleton and transmembrane proteins. The ERM proteins are differentially expressed in the RMS with radixin and moesin localized to neuroblasts, and ezrin expression confined to astrocytes of the glial tubes. Here, we inhibited radixin function using the quinocarmycin analog DX52-1 which resulted in reduced neuroblast migration in vitro, while glial migration remained unaltered. Furthermore, the morphology of neuroblasts was distorted resulting in a rounded shape with no or short polysialylated neural cell adhesion molecule positive processes. Intracerebroventricular infusion of the radixin inhibitor resulted in accumulation of neuroblasts in the anterior SVZ. Neuroblast chains were short and intermittently interrupted in the SVZ and considerably disorganized in the RMS. Moreover, we studied the proliferation activity in the RMS after radixin inhibition, since concentrated radixin expression has been demonstrated in the cleavage furrow of dividing cells, which indicates a role of radixin in cell division. Radixin inhibition decreased neuroblast proliferation, whereas the proliferation of other cells in the RMS was not affected. Our results demonstrate a significant role for radixin in neuroblast proliferation and migration. |
format | Online Article Text |
id | pubmed-3781578 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-37815782013-09-24 Radixin inhibition decreases adult neural progenitor cell migration and proliferation in vitro and in vivo Persson, Åsa Lindberg, Olle R. Kuhn, Hans G. Front Cell Neurosci Neuroscience Neuronal progenitors capable of long distance migration are produced throughout life in the subventricular zone (SVZ). Migration from the SVZ is carried out along a well-defined pathway called the rostral migratory stream (RMS). Our recent finding of the specific expression of the cytoskeleton linker protein radixin in neuroblasts suggests a functional role for radixin in RMS migration. The ezrin-radixin-moesin (ERM) family of proteins is capable of regulating migration through interaction with the actin cytoskeleton and transmembrane proteins. The ERM proteins are differentially expressed in the RMS with radixin and moesin localized to neuroblasts, and ezrin expression confined to astrocytes of the glial tubes. Here, we inhibited radixin function using the quinocarmycin analog DX52-1 which resulted in reduced neuroblast migration in vitro, while glial migration remained unaltered. Furthermore, the morphology of neuroblasts was distorted resulting in a rounded shape with no or short polysialylated neural cell adhesion molecule positive processes. Intracerebroventricular infusion of the radixin inhibitor resulted in accumulation of neuroblasts in the anterior SVZ. Neuroblast chains were short and intermittently interrupted in the SVZ and considerably disorganized in the RMS. Moreover, we studied the proliferation activity in the RMS after radixin inhibition, since concentrated radixin expression has been demonstrated in the cleavage furrow of dividing cells, which indicates a role of radixin in cell division. Radixin inhibition decreased neuroblast proliferation, whereas the proliferation of other cells in the RMS was not affected. Our results demonstrate a significant role for radixin in neuroblast proliferation and migration. Frontiers Media S.A. 2013-09-24 /pmc/articles/PMC3781578/ /pubmed/24065889 http://dx.doi.org/10.3389/fncel.2013.00161 Text en Copyright © Persson, Lindberg and Kuhn. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroscience Persson, Åsa Lindberg, Olle R. Kuhn, Hans G. Radixin inhibition decreases adult neural progenitor cell migration and proliferation in vitro and in vivo |
title | Radixin inhibition decreases adult neural progenitor cell migration and proliferation in vitro and in vivo |
title_full | Radixin inhibition decreases adult neural progenitor cell migration and proliferation in vitro and in vivo |
title_fullStr | Radixin inhibition decreases adult neural progenitor cell migration and proliferation in vitro and in vivo |
title_full_unstemmed | Radixin inhibition decreases adult neural progenitor cell migration and proliferation in vitro and in vivo |
title_short | Radixin inhibition decreases adult neural progenitor cell migration and proliferation in vitro and in vivo |
title_sort | radixin inhibition decreases adult neural progenitor cell migration and proliferation in vitro and in vivo |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3781578/ https://www.ncbi.nlm.nih.gov/pubmed/24065889 http://dx.doi.org/10.3389/fncel.2013.00161 |
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