Gene transfection in primary stem-like cells of giant cell tumor of bone

The neoplastic stem-like stromal cell of giant cell tumor of bone (GCT) survives for multiple passages in primary culture with a stable phenotype, and exhibits multipotent characteristics. The pathophysiology of this tumor has been studied through the primary culture of these cells. However, success...

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Autores principales: Singh, Shalini, Mak, Isabella, Power, Patricia, Cunnigham, Melissa, Turcotte, Robert, Ghert, Michelle
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2010
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3781737/
https://www.ncbi.nlm.nih.gov/pubmed/24198518
http://dx.doi.org/10.2147/SCCAA.S14265
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author Singh, Shalini
Mak, Isabella
Power, Patricia
Cunnigham, Melissa
Turcotte, Robert
Ghert, Michelle
author_facet Singh, Shalini
Mak, Isabella
Power, Patricia
Cunnigham, Melissa
Turcotte, Robert
Ghert, Michelle
author_sort Singh, Shalini
collection PubMed
description The neoplastic stem-like stromal cell of giant cell tumor of bone (GCT) survives for multiple passages in primary culture with a stable phenotype, and exhibits multipotent characteristics. The pathophysiology of this tumor has been studied through the primary culture of these cells. However, successful gene transfer of these cells has not been reported to date. In this short report, we describe the development of the first reported technique that results in efficient gene transfection in primary stem-like cells of GCT.
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spelling pubmed-37817372013-11-06 Gene transfection in primary stem-like cells of giant cell tumor of bone Singh, Shalini Mak, Isabella Power, Patricia Cunnigham, Melissa Turcotte, Robert Ghert, Michelle Stem Cells Cloning Short Report The neoplastic stem-like stromal cell of giant cell tumor of bone (GCT) survives for multiple passages in primary culture with a stable phenotype, and exhibits multipotent characteristics. The pathophysiology of this tumor has been studied through the primary culture of these cells. However, successful gene transfer of these cells has not been reported to date. In this short report, we describe the development of the first reported technique that results in efficient gene transfection in primary stem-like cells of GCT. Dove Medical Press 2010-09-28 /pmc/articles/PMC3781737/ /pubmed/24198518 http://dx.doi.org/10.2147/SCCAA.S14265 Text en © 2010 Singh et al, publisher and licensee Dove Medical Press Ltd This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.
spellingShingle Short Report
Singh, Shalini
Mak, Isabella
Power, Patricia
Cunnigham, Melissa
Turcotte, Robert
Ghert, Michelle
Gene transfection in primary stem-like cells of giant cell tumor of bone
title Gene transfection in primary stem-like cells of giant cell tumor of bone
title_full Gene transfection in primary stem-like cells of giant cell tumor of bone
title_fullStr Gene transfection in primary stem-like cells of giant cell tumor of bone
title_full_unstemmed Gene transfection in primary stem-like cells of giant cell tumor of bone
title_short Gene transfection in primary stem-like cells of giant cell tumor of bone
title_sort gene transfection in primary stem-like cells of giant cell tumor of bone
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3781737/
https://www.ncbi.nlm.nih.gov/pubmed/24198518
http://dx.doi.org/10.2147/SCCAA.S14265
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