Cargando…

Intestinal lamina propria dendritic cells maintain T cell homeostasis but do not affect commensalism

Dendritic cells (DCs) in the intestinal lamina propria (LP) are composed of two CD103(+) subsets that differ in CD11b expression. We report here that Langerin is expressed by human LP DCs and that transgenic human langerin drives expression in CD103(+)CD11b(+) LP DCs in mice. This subset was ablated...

Descripción completa

Detalles Bibliográficos
Autores principales: Welty, Nathan E., Staley, Christopher, Ghilardi, Nico, Sadowsky, Michael J., Igyártó, Botond Z., Kaplan, Daniel H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3782055/
https://www.ncbi.nlm.nih.gov/pubmed/24019552
http://dx.doi.org/10.1084/jem.20130728
_version_ 1782285512027930624
author Welty, Nathan E.
Staley, Christopher
Ghilardi, Nico
Sadowsky, Michael J.
Igyártó, Botond Z.
Kaplan, Daniel H.
author_facet Welty, Nathan E.
Staley, Christopher
Ghilardi, Nico
Sadowsky, Michael J.
Igyártó, Botond Z.
Kaplan, Daniel H.
author_sort Welty, Nathan E.
collection PubMed
description Dendritic cells (DCs) in the intestinal lamina propria (LP) are composed of two CD103(+) subsets that differ in CD11b expression. We report here that Langerin is expressed by human LP DCs and that transgenic human langerin drives expression in CD103(+)CD11b(+) LP DCs in mice. This subset was ablated in huLangerin-DTA mice, resulting in reduced LP Th17 cells without affecting Th1 or T reg cells. Notably, cognate DC–T cell interactions were not required for Th17 development, as this response was intact in huLangerin-Cre I-Aβ(fl/fl) mice. In contrast, responses to intestinal infection or flagellin administration were unaffected by the absence of CD103(+)CD11b(+) DCs. huLangerin-DTA x BatF3(−/−) mice lacked both CD103(+) LP DC subsets, resulting in defective gut homing and fewer LP T reg cells. Despite these defects in LP DCs and resident T cells, we did not observe alterations of intestinal microbial communities. Thus, CD103(+) LP DC subsets control T cell homeostasis through both nonredundant and overlapping mechanisms.
format Online
Article
Text
id pubmed-3782055
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-37820552014-03-23 Intestinal lamina propria dendritic cells maintain T cell homeostasis but do not affect commensalism Welty, Nathan E. Staley, Christopher Ghilardi, Nico Sadowsky, Michael J. Igyártó, Botond Z. Kaplan, Daniel H. J Exp Med Article Dendritic cells (DCs) in the intestinal lamina propria (LP) are composed of two CD103(+) subsets that differ in CD11b expression. We report here that Langerin is expressed by human LP DCs and that transgenic human langerin drives expression in CD103(+)CD11b(+) LP DCs in mice. This subset was ablated in huLangerin-DTA mice, resulting in reduced LP Th17 cells without affecting Th1 or T reg cells. Notably, cognate DC–T cell interactions were not required for Th17 development, as this response was intact in huLangerin-Cre I-Aβ(fl/fl) mice. In contrast, responses to intestinal infection or flagellin administration were unaffected by the absence of CD103(+)CD11b(+) DCs. huLangerin-DTA x BatF3(−/−) mice lacked both CD103(+) LP DC subsets, resulting in defective gut homing and fewer LP T reg cells. Despite these defects in LP DCs and resident T cells, we did not observe alterations of intestinal microbial communities. Thus, CD103(+) LP DC subsets control T cell homeostasis through both nonredundant and overlapping mechanisms. The Rockefeller University Press 2013-09-23 /pmc/articles/PMC3782055/ /pubmed/24019552 http://dx.doi.org/10.1084/jem.20130728 Text en © 2013 Welty et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Article
Welty, Nathan E.
Staley, Christopher
Ghilardi, Nico
Sadowsky, Michael J.
Igyártó, Botond Z.
Kaplan, Daniel H.
Intestinal lamina propria dendritic cells maintain T cell homeostasis but do not affect commensalism
title Intestinal lamina propria dendritic cells maintain T cell homeostasis but do not affect commensalism
title_full Intestinal lamina propria dendritic cells maintain T cell homeostasis but do not affect commensalism
title_fullStr Intestinal lamina propria dendritic cells maintain T cell homeostasis but do not affect commensalism
title_full_unstemmed Intestinal lamina propria dendritic cells maintain T cell homeostasis but do not affect commensalism
title_short Intestinal lamina propria dendritic cells maintain T cell homeostasis but do not affect commensalism
title_sort intestinal lamina propria dendritic cells maintain t cell homeostasis but do not affect commensalism
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3782055/
https://www.ncbi.nlm.nih.gov/pubmed/24019552
http://dx.doi.org/10.1084/jem.20130728
work_keys_str_mv AT weltynathane intestinallaminapropriadendriticcellsmaintaintcellhomeostasisbutdonotaffectcommensalism
AT staleychristopher intestinallaminapropriadendriticcellsmaintaintcellhomeostasisbutdonotaffectcommensalism
AT ghilardinico intestinallaminapropriadendriticcellsmaintaintcellhomeostasisbutdonotaffectcommensalism
AT sadowskymichaelj intestinallaminapropriadendriticcellsmaintaintcellhomeostasisbutdonotaffectcommensalism
AT igyartobotondz intestinallaminapropriadendriticcellsmaintaintcellhomeostasisbutdonotaffectcommensalism
AT kaplandanielh intestinallaminapropriadendriticcellsmaintaintcellhomeostasisbutdonotaffectcommensalism