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Toxoplasma gondii Soluble Tachyzoite Antigen Triggers Protective Mechanisms against Fatal Intestinal Pathology in Oral Infection of C57BL/6 Mice

Toxoplasma gondii induces a potent IL-12 response early in infection that results in IFN-γ-dependent control of parasite growth. It was previously shown that T. gondii soluble tachyzoite antigen (STAg) injected 48 hr before intraperitoneal infection reduces lipoxin A(4) and 5-lipoxygenase (5-LO)-dep...

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Autores principales: Benevides, Luciana, Cardoso, Cristina R., Milanezi, Cristiane M., Castro-Filice, Letícia S., Barenco, Paulo V. C., Sousa, Romulo O., Rodrigues, Rosangela M., Mineo, José R., Silva, João S., Silva, Neide M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3782460/
https://www.ncbi.nlm.nih.gov/pubmed/24086456
http://dx.doi.org/10.1371/journal.pone.0075138
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author Benevides, Luciana
Cardoso, Cristina R.
Milanezi, Cristiane M.
Castro-Filice, Letícia S.
Barenco, Paulo V. C.
Sousa, Romulo O.
Rodrigues, Rosangela M.
Mineo, José R.
Silva, João S.
Silva, Neide M.
author_facet Benevides, Luciana
Cardoso, Cristina R.
Milanezi, Cristiane M.
Castro-Filice, Letícia S.
Barenco, Paulo V. C.
Sousa, Romulo O.
Rodrigues, Rosangela M.
Mineo, José R.
Silva, João S.
Silva, Neide M.
author_sort Benevides, Luciana
collection PubMed
description Toxoplasma gondii induces a potent IL-12 response early in infection that results in IFN-γ-dependent control of parasite growth. It was previously shown that T. gondii soluble tachyzoite antigen (STAg) injected 48 hr before intraperitoneal infection reduces lipoxin A(4) and 5-lipoxygenase (5-LO)-dependent systemic IL-12 and IFN-γ production as well as hepatic immunopathology. This study investigated the ability of STAg-pretreatment to control the fatal intestinal pathology that develops in C57BL/6 mice orally infected with 100 T. gondii cysts. STAg-pretreatment prolonged the animals’ survival by decreasing tissue parasitism and pathology, mainly in the ilea. Protection was associated with decreases in the systemic IFN-γ levels and IFN-γ and TNF message levels in the ilea and with increased TGF-β production in this tissue, but protection was independent of 5-LO and IL-4. STAg-pretreatment decreased CD4(+) T cell, NK cell, CD11b(+) monocyte and CD11b(+)CD11c(+) dendritic cell numbers in the lamina propria and increased CD8(+) T cells in the intestinal epithelial compartment. In parallel, decreases were observed in iNOS and IL-17 expression in this organ. These results demonstrate that pretreatment with STAg can induce the recruitment of protective CD8(+) T cells to the intraepithelial compartment and decrease proinflammatory immune mechanisms that promote intestinal pathology in T. gondii infection.
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spelling pubmed-37824602013-10-01 Toxoplasma gondii Soluble Tachyzoite Antigen Triggers Protective Mechanisms against Fatal Intestinal Pathology in Oral Infection of C57BL/6 Mice Benevides, Luciana Cardoso, Cristina R. Milanezi, Cristiane M. Castro-Filice, Letícia S. Barenco, Paulo V. C. Sousa, Romulo O. Rodrigues, Rosangela M. Mineo, José R. Silva, João S. Silva, Neide M. PLoS One Research Article Toxoplasma gondii induces a potent IL-12 response early in infection that results in IFN-γ-dependent control of parasite growth. It was previously shown that T. gondii soluble tachyzoite antigen (STAg) injected 48 hr before intraperitoneal infection reduces lipoxin A(4) and 5-lipoxygenase (5-LO)-dependent systemic IL-12 and IFN-γ production as well as hepatic immunopathology. This study investigated the ability of STAg-pretreatment to control the fatal intestinal pathology that develops in C57BL/6 mice orally infected with 100 T. gondii cysts. STAg-pretreatment prolonged the animals’ survival by decreasing tissue parasitism and pathology, mainly in the ilea. Protection was associated with decreases in the systemic IFN-γ levels and IFN-γ and TNF message levels in the ilea and with increased TGF-β production in this tissue, but protection was independent of 5-LO and IL-4. STAg-pretreatment decreased CD4(+) T cell, NK cell, CD11b(+) monocyte and CD11b(+)CD11c(+) dendritic cell numbers in the lamina propria and increased CD8(+) T cells in the intestinal epithelial compartment. In parallel, decreases were observed in iNOS and IL-17 expression in this organ. These results demonstrate that pretreatment with STAg can induce the recruitment of protective CD8(+) T cells to the intraepithelial compartment and decrease proinflammatory immune mechanisms that promote intestinal pathology in T. gondii infection. Public Library of Science 2013-09-24 /pmc/articles/PMC3782460/ /pubmed/24086456 http://dx.doi.org/10.1371/journal.pone.0075138 Text en © 2013 Benevides et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Benevides, Luciana
Cardoso, Cristina R.
Milanezi, Cristiane M.
Castro-Filice, Letícia S.
Barenco, Paulo V. C.
Sousa, Romulo O.
Rodrigues, Rosangela M.
Mineo, José R.
Silva, João S.
Silva, Neide M.
Toxoplasma gondii Soluble Tachyzoite Antigen Triggers Protective Mechanisms against Fatal Intestinal Pathology in Oral Infection of C57BL/6 Mice
title Toxoplasma gondii Soluble Tachyzoite Antigen Triggers Protective Mechanisms against Fatal Intestinal Pathology in Oral Infection of C57BL/6 Mice
title_full Toxoplasma gondii Soluble Tachyzoite Antigen Triggers Protective Mechanisms against Fatal Intestinal Pathology in Oral Infection of C57BL/6 Mice
title_fullStr Toxoplasma gondii Soluble Tachyzoite Antigen Triggers Protective Mechanisms against Fatal Intestinal Pathology in Oral Infection of C57BL/6 Mice
title_full_unstemmed Toxoplasma gondii Soluble Tachyzoite Antigen Triggers Protective Mechanisms against Fatal Intestinal Pathology in Oral Infection of C57BL/6 Mice
title_short Toxoplasma gondii Soluble Tachyzoite Antigen Triggers Protective Mechanisms against Fatal Intestinal Pathology in Oral Infection of C57BL/6 Mice
title_sort toxoplasma gondii soluble tachyzoite antigen triggers protective mechanisms against fatal intestinal pathology in oral infection of c57bl/6 mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3782460/
https://www.ncbi.nlm.nih.gov/pubmed/24086456
http://dx.doi.org/10.1371/journal.pone.0075138
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