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Utilization of transposable element mPing as a novel genetic tool for modification of the stress response in rice

Transposable elements (TEs) are DNA fragments that have the ability to move from one chromosomal location to another. The insertion of TEs into gene-rich regions often affects changes in the expression of neighboring genes. Miniature Ping (mPing) is an active miniature inverted-repeat TE discovered...

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Autores principales: Yasuda, Kanako, Ito, Makoto, Sugita, Tomohiko, Tsukiyama, Takuji, Saito, Hiroki, Naito, Ken, Teraishi, Masayoshi, Tanisaka, Takatoshi, Okumoto, Yutaka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3782648/
https://www.ncbi.nlm.nih.gov/pubmed/24078785
http://dx.doi.org/10.1007/s11032-013-9885-1
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author Yasuda, Kanako
Ito, Makoto
Sugita, Tomohiko
Tsukiyama, Takuji
Saito, Hiroki
Naito, Ken
Teraishi, Masayoshi
Tanisaka, Takatoshi
Okumoto, Yutaka
author_facet Yasuda, Kanako
Ito, Makoto
Sugita, Tomohiko
Tsukiyama, Takuji
Saito, Hiroki
Naito, Ken
Teraishi, Masayoshi
Tanisaka, Takatoshi
Okumoto, Yutaka
author_sort Yasuda, Kanako
collection PubMed
description Transposable elements (TEs) are DNA fragments that have the ability to move from one chromosomal location to another. The insertion of TEs into gene-rich regions often affects changes in the expression of neighboring genes. Miniature Ping (mPing) is an active miniature inverted-repeat TE discovered in the rice genome. It has been found to show exceptionally active transposition in a few japonica rice varieties, including Gimbozu, where mPing insertion rendered adjacent genes stress-inducible. In the Gimbozu population, it is highly possible that several genes with modified expression profiles are segregating due to the de novo mPing insertions. In our study, we utilized a screening system for detecting de novo mPing insertions in the upstream region of target genes and evaluated the effect of mPing on the stress response of the target genes. Screening for 17 targeted genes revealed five genes with the mPing insertion in their promoters. In most cases, the alteration of gene expression was observed under stress conditions, and there was no change in the expression levels of those five genes under normal conditions. These results indicate that the mPing insertion can be used as a genetic tool to modify an expression pattern of a target gene under stress conditions without changing the expression profiles of those under natural conditions. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11032-013-9885-1) contains supplementary material, which is available to authorized users.
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spelling pubmed-37826482013-09-25 Utilization of transposable element mPing as a novel genetic tool for modification of the stress response in rice Yasuda, Kanako Ito, Makoto Sugita, Tomohiko Tsukiyama, Takuji Saito, Hiroki Naito, Ken Teraishi, Masayoshi Tanisaka, Takatoshi Okumoto, Yutaka Mol Breed Article Transposable elements (TEs) are DNA fragments that have the ability to move from one chromosomal location to another. The insertion of TEs into gene-rich regions often affects changes in the expression of neighboring genes. Miniature Ping (mPing) is an active miniature inverted-repeat TE discovered in the rice genome. It has been found to show exceptionally active transposition in a few japonica rice varieties, including Gimbozu, where mPing insertion rendered adjacent genes stress-inducible. In the Gimbozu population, it is highly possible that several genes with modified expression profiles are segregating due to the de novo mPing insertions. In our study, we utilized a screening system for detecting de novo mPing insertions in the upstream region of target genes and evaluated the effect of mPing on the stress response of the target genes. Screening for 17 targeted genes revealed five genes with the mPing insertion in their promoters. In most cases, the alteration of gene expression was observed under stress conditions, and there was no change in the expression levels of those five genes under normal conditions. These results indicate that the mPing insertion can be used as a genetic tool to modify an expression pattern of a target gene under stress conditions without changing the expression profiles of those under natural conditions. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11032-013-9885-1) contains supplementary material, which is available to authorized users. Springer Netherlands 2013-06-08 2013 /pmc/articles/PMC3782648/ /pubmed/24078785 http://dx.doi.org/10.1007/s11032-013-9885-1 Text en © The Author(s) 2013 https://creativecommons.org/licenses/by/2.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Article
Yasuda, Kanako
Ito, Makoto
Sugita, Tomohiko
Tsukiyama, Takuji
Saito, Hiroki
Naito, Ken
Teraishi, Masayoshi
Tanisaka, Takatoshi
Okumoto, Yutaka
Utilization of transposable element mPing as a novel genetic tool for modification of the stress response in rice
title Utilization of transposable element mPing as a novel genetic tool for modification of the stress response in rice
title_full Utilization of transposable element mPing as a novel genetic tool for modification of the stress response in rice
title_fullStr Utilization of transposable element mPing as a novel genetic tool for modification of the stress response in rice
title_full_unstemmed Utilization of transposable element mPing as a novel genetic tool for modification of the stress response in rice
title_short Utilization of transposable element mPing as a novel genetic tool for modification of the stress response in rice
title_sort utilization of transposable element mping as a novel genetic tool for modification of the stress response in rice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3782648/
https://www.ncbi.nlm.nih.gov/pubmed/24078785
http://dx.doi.org/10.1007/s11032-013-9885-1
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