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Thymosin ß(4) expression in colorectal polyps and adenomas

OBJECTIVE: Thymosin beta 4 (Tβ4) is a ubiquitous peptide that plays pivotal roles in the cytoskeletal system and in cell differentiation. Recently, a role for Tβ4 has been proposed in experimental and human carcinogenesis, including gastrointestinal cancer. This study was aimed at evaluating the rel...

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Autores principales: Nemolato, Sonia, Cabras, Tiziana, Restivo, Angelo, Zorcolo, Luigi, Di Felice, Eliana, Fanni, Daniela, Gerosa, Clara, Messana, Irene, Castagnola, Massimo, Faa, Gavino, Casula, Giuseppe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3782725/
https://www.ncbi.nlm.nih.gov/pubmed/24141838
http://dx.doi.org/10.6061/clinics/2013(09)08
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author Nemolato, Sonia
Cabras, Tiziana
Restivo, Angelo
Zorcolo, Luigi
Di Felice, Eliana
Fanni, Daniela
Gerosa, Clara
Messana, Irene
Castagnola, Massimo
Faa, Gavino
Casula, Giuseppe
author_facet Nemolato, Sonia
Cabras, Tiziana
Restivo, Angelo
Zorcolo, Luigi
Di Felice, Eliana
Fanni, Daniela
Gerosa, Clara
Messana, Irene
Castagnola, Massimo
Faa, Gavino
Casula, Giuseppe
author_sort Nemolato, Sonia
collection PubMed
description OBJECTIVE: Thymosin beta 4 (Tβ4) is a ubiquitous peptide that plays pivotal roles in the cytoskeletal system and in cell differentiation. Recently, a role for Tβ4 has been proposed in experimental and human carcinogenesis, including gastrointestinal cancer. This study was aimed at evaluating the relationship between Tβ4 immunoreactivity and the initial steps of carcinogenesis. METHODS: In total, 60 intestinal biopsies, including 10 hyperplastic polyps, 10 sessile serrated adenomas/polyps, 15 colorectal adenomas with low-grade dysplasia, 15 adenomas with high-grade dysplasia, 15 adenocarcinomas and 10 samples of normal colon mucosa, were analyzed for Tβ4 expression by immunohistochemistry. RESULTS: Weak cytoplasmic reactivity for Tβ4 was detected in the normal colon mucosa. No reactivity for Tβ4 was found in hyperplastic and sessile serrated polyps/adenomas. Tβ4 expression was observed in 10/15 colorectal adenocarcinomas. In adenomas with low-grade dysplasia, Tβ4 immunoreactivity was mainly detected in dysplastic glands but was absent in hyperplastic glands. Tβ4 immunoreactivity was characterized by spot-like perinuclear staining. In high-grade dysplastic polyps, immunostaining for Tβ4 appeared diffuse throughout the entire cytoplasm of dysplastic cells. Spot-like perinuclear reactivity was detected in adenocarcinoma tumor cells. CONCLUSIONS: Our study shows for the first time that Tβ4 is expressed during different steps of colon carcinogenesis. The shift of Tβ4 immunolocalization from low-grade to high-grade dysplastic glands suggests a role for Tβ4 in colorectal carcinogenesis. However, the real meaning of Tβ4 reactivity in dysplastic intestinal epithelium remains unknown.
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spelling pubmed-37827252013-09-25 Thymosin ß(4) expression in colorectal polyps and adenomas Nemolato, Sonia Cabras, Tiziana Restivo, Angelo Zorcolo, Luigi Di Felice, Eliana Fanni, Daniela Gerosa, Clara Messana, Irene Castagnola, Massimo Faa, Gavino Casula, Giuseppe Clinics (Sao Paulo) Clinical Science OBJECTIVE: Thymosin beta 4 (Tβ4) is a ubiquitous peptide that plays pivotal roles in the cytoskeletal system and in cell differentiation. Recently, a role for Tβ4 has been proposed in experimental and human carcinogenesis, including gastrointestinal cancer. This study was aimed at evaluating the relationship between Tβ4 immunoreactivity and the initial steps of carcinogenesis. METHODS: In total, 60 intestinal biopsies, including 10 hyperplastic polyps, 10 sessile serrated adenomas/polyps, 15 colorectal adenomas with low-grade dysplasia, 15 adenomas with high-grade dysplasia, 15 adenocarcinomas and 10 samples of normal colon mucosa, were analyzed for Tβ4 expression by immunohistochemistry. RESULTS: Weak cytoplasmic reactivity for Tβ4 was detected in the normal colon mucosa. No reactivity for Tβ4 was found in hyperplastic and sessile serrated polyps/adenomas. Tβ4 expression was observed in 10/15 colorectal adenocarcinomas. In adenomas with low-grade dysplasia, Tβ4 immunoreactivity was mainly detected in dysplastic glands but was absent in hyperplastic glands. Tβ4 immunoreactivity was characterized by spot-like perinuclear staining. In high-grade dysplastic polyps, immunostaining for Tβ4 appeared diffuse throughout the entire cytoplasm of dysplastic cells. Spot-like perinuclear reactivity was detected in adenocarcinoma tumor cells. CONCLUSIONS: Our study shows for the first time that Tβ4 is expressed during different steps of colon carcinogenesis. The shift of Tβ4 immunolocalization from low-grade to high-grade dysplastic glands suggests a role for Tβ4 in colorectal carcinogenesis. However, the real meaning of Tβ4 reactivity in dysplastic intestinal epithelium remains unknown. Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo 2013-09 /pmc/articles/PMC3782725/ /pubmed/24141838 http://dx.doi.org/10.6061/clinics/2013(09)08 Text en Copyright © 2013 Hospital das Clínicas da FMUSP http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Clinical Science
Nemolato, Sonia
Cabras, Tiziana
Restivo, Angelo
Zorcolo, Luigi
Di Felice, Eliana
Fanni, Daniela
Gerosa, Clara
Messana, Irene
Castagnola, Massimo
Faa, Gavino
Casula, Giuseppe
Thymosin ß(4) expression in colorectal polyps and adenomas
title Thymosin ß(4) expression in colorectal polyps and adenomas
title_full Thymosin ß(4) expression in colorectal polyps and adenomas
title_fullStr Thymosin ß(4) expression in colorectal polyps and adenomas
title_full_unstemmed Thymosin ß(4) expression in colorectal polyps and adenomas
title_short Thymosin ß(4) expression in colorectal polyps and adenomas
title_sort thymosin ß(4) expression in colorectal polyps and adenomas
topic Clinical Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3782725/
https://www.ncbi.nlm.nih.gov/pubmed/24141838
http://dx.doi.org/10.6061/clinics/2013(09)08
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