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A new strategy for gene targeting and functional proteomics using the DT40 cell line
DT40 cells derived from chicken B lymphocytes exhibit exceptionally high homologous recombination rates. Therefore, they can be used as a convenient tool and model for gene targeting experiments. However, lack of efficient cloning strategies, protein purification protocols and a well annotated prote...
Autores principales: | Orlowska, Kinga P., Klosowska, Kamila, Szczesny, Roman J., Cysewski, Dominik, Krawczyk, Pawel S., Dziembowski, Andrzej |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3783193/ https://www.ncbi.nlm.nih.gov/pubmed/23892402 http://dx.doi.org/10.1093/nar/gkt650 |
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