Gender and Timing during Ontogeny Matter: Effects of a Temporary High Temperature on Survival, Body Size and Colouration in Harmonia axyridis

The ambient temperature experienced during development is a crucial factor affecting survival and adult phenotype in ectotherms. Moreover, the exact response of individuals to different temperature regimes is frequently sex-specific. This sex-specific response can result in varying levels of sexual dimorphism according to the experienced conditions. The majority of studies have investigated the effects of temperature on individuals reared under a constant temperature regime throughout their whole preimaginal development, whereas information on stage-dependent variation in temperature effects is scarce. Here we investigate how the stage at which elevated temperature is experienced influences survival, adult body size and colouration in the harlequin ladybird Harmonia axyridis form succinea. The effects of timing of exposure to elevated temperature on the adult phenotype are assessed separately for males and females. Control individuals were reared at a constant temperature of 20°C. Beetles in other treatments were additionally exposed to 33°C for 48 hours during the following developmental stages: egg, 1(st) to 2(nd) larval instar, 3(rd) larval instar, 4(th) larval instar and pupa. Exposure to an elevated temperature during the early developmental stages resulted in lower survival, but the adult phenotype of survivors was almost unaffected. Exposure to an elevated temperature during the later developmental stages (4(th) larval instar or pupa) resulted in the decreased melanisation of elytra, decreased structural body size and increased dry mass. Furthermore, the timing of high temperature exposure affected the degree of sexual dimorphism in elytral melanisation and dry mass. We demonstrate that the effects of elevated temperature can vary according to the developmental stage at exposure. Detailed information on how ambient temperature affects the developmental biology of ectotherms is crucial for modeling population growth and predicting the spread of invasive species such as Harmonia axyridis.