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MiR-27a Regulates Apoptosis in Nucleus Pulposus Cells by Targeting PI3K

The precise role of apoptosis in the pathogenesis of intervertebral disc degeneration (IDD) remains to be elucidated. We analyzed degenerative nucleus pulposus (NP) cells and found that the expression of miR-27a was increased. The overexpression of miR-27a was further verified using real-time RT-PCR...

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Autores principales: Liu, Gang, Cao, Peng, Chen, Huajiang, Yuan, Wen, Wang, Jianxi, Tang, Xianye
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3783482/
https://www.ncbi.nlm.nih.gov/pubmed/24086481
http://dx.doi.org/10.1371/journal.pone.0075251
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author Liu, Gang
Cao, Peng
Chen, Huajiang
Yuan, Wen
Wang, Jianxi
Tang, Xianye
author_facet Liu, Gang
Cao, Peng
Chen, Huajiang
Yuan, Wen
Wang, Jianxi
Tang, Xianye
author_sort Liu, Gang
collection PubMed
description The precise role of apoptosis in the pathogenesis of intervertebral disc degeneration (IDD) remains to be elucidated. We analyzed degenerative nucleus pulposus (NP) cells and found that the expression of miR-27a was increased. The overexpression of miR-27a was further verified using real-time RT-PCR. Bioinformatics target prediction identified phosphoinositide-3 kinases (PI3K) as putative targets of miR-27a. Furthermore, miR-27a inhibited PI3K expression by directly targeting their 3’-UTRs, and this inhibition was abolished by mutation of the miR-27a binding sites. Various cellular processes including cell growth, proliferation, migration and adhesion are regulated by activation of the PI3K/AKT signaling pathway, and nucleus pulposus cells are known to strongly express the phosphorylated survival protein AKT. Our results identify PI3K as a novel target of miR-27a. Upregulation of miR-27a thus targets PI3K, initiating apoptosis of nucleus pulposus cells. This present study revealed that downregulated miR-27a might develop a novel intervention for IDD treatment through the prevention of apoptosis in Nucleus pulposus Cells.
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spelling pubmed-37834822013-10-01 MiR-27a Regulates Apoptosis in Nucleus Pulposus Cells by Targeting PI3K Liu, Gang Cao, Peng Chen, Huajiang Yuan, Wen Wang, Jianxi Tang, Xianye PLoS One Research Article The precise role of apoptosis in the pathogenesis of intervertebral disc degeneration (IDD) remains to be elucidated. We analyzed degenerative nucleus pulposus (NP) cells and found that the expression of miR-27a was increased. The overexpression of miR-27a was further verified using real-time RT-PCR. Bioinformatics target prediction identified phosphoinositide-3 kinases (PI3K) as putative targets of miR-27a. Furthermore, miR-27a inhibited PI3K expression by directly targeting their 3’-UTRs, and this inhibition was abolished by mutation of the miR-27a binding sites. Various cellular processes including cell growth, proliferation, migration and adhesion are regulated by activation of the PI3K/AKT signaling pathway, and nucleus pulposus cells are known to strongly express the phosphorylated survival protein AKT. Our results identify PI3K as a novel target of miR-27a. Upregulation of miR-27a thus targets PI3K, initiating apoptosis of nucleus pulposus cells. This present study revealed that downregulated miR-27a might develop a novel intervention for IDD treatment through the prevention of apoptosis in Nucleus pulposus Cells. Public Library of Science 2013-09-25 /pmc/articles/PMC3783482/ /pubmed/24086481 http://dx.doi.org/10.1371/journal.pone.0075251 Text en © 2013 Liu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Liu, Gang
Cao, Peng
Chen, Huajiang
Yuan, Wen
Wang, Jianxi
Tang, Xianye
MiR-27a Regulates Apoptosis in Nucleus Pulposus Cells by Targeting PI3K
title MiR-27a Regulates Apoptosis in Nucleus Pulposus Cells by Targeting PI3K
title_full MiR-27a Regulates Apoptosis in Nucleus Pulposus Cells by Targeting PI3K
title_fullStr MiR-27a Regulates Apoptosis in Nucleus Pulposus Cells by Targeting PI3K
title_full_unstemmed MiR-27a Regulates Apoptosis in Nucleus Pulposus Cells by Targeting PI3K
title_short MiR-27a Regulates Apoptosis in Nucleus Pulposus Cells by Targeting PI3K
title_sort mir-27a regulates apoptosis in nucleus pulposus cells by targeting pi3k
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3783482/
https://www.ncbi.nlm.nih.gov/pubmed/24086481
http://dx.doi.org/10.1371/journal.pone.0075251
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