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Phytochemical study and screening for antimicrobial activity of flavonoids of Euphorbia hirta

OBJECTIVE: This study aims to phytochemical and antimicrobial study of Euphorbia hirta Euphorbiaceae). MATERIALS AND METHODS: Antimicrobial activity of flavonoids (free and bound) of Euphorbia hirta L. was determined by disc diffusion assay against four bacteria (Escherichia coli, Pseudomonas aerugi...

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Autores principales: Singh, Geeta, Kumar, Padma
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3783663/
https://www.ncbi.nlm.nih.gov/pubmed/24083146
http://dx.doi.org/10.4103/2229-516X.117082
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author Singh, Geeta
Kumar, Padma
author_facet Singh, Geeta
Kumar, Padma
author_sort Singh, Geeta
collection PubMed
description OBJECTIVE: This study aims to phytochemical and antimicrobial study of Euphorbia hirta Euphorbiaceae). MATERIALS AND METHODS: Antimicrobial activity of flavonoids (free and bound) of Euphorbia hirta L. was determined by disc diffusion assay against four bacteria (Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis, and Staphylococcus aureus) and four fungi (Aspergillus flavus, Aspergillus niger, Trichophyton mentagrophytes, and Candida albicans). Minimum inhibitory concentration (MIC) of the extract was evaluated through micro broth dilution method, while minimum bactericidal/fungicidal concentration was determined by subculturing the relevant samples. Total activity (TA) of extracts against each sensitive pathogen was also evaluated. RESULTS: Out of fungi; A. flavus, A. niger, and T. mentagrophytes were found to be resistant, against which none of the tested extracts showed activity. Bound flavonoids extract of root showed best activity against C. albicans (inhibition zone (IZ) 27.66, MIC 0.039, minimum fungicidal concentration (MFC) 0.039). TA of free flavonoid extract of root was found to be the same for P. mirabilis and S. aureus (192.30 ml/g). Two flavonoids quercetin and kaempferol were identified in the bound flavonoids of stem extract which showed activity against all the microorganisms. CONCLUSION: Results of the present investigation indicate that E. hirta has good antimicrobial activity with low range of MIC, hence can be exploited for future plant-based antimicrobial drugs.
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spelling pubmed-37836632013-09-30 Phytochemical study and screening for antimicrobial activity of flavonoids of Euphorbia hirta Singh, Geeta Kumar, Padma Int J Appl Basic Med Res Original Article OBJECTIVE: This study aims to phytochemical and antimicrobial study of Euphorbia hirta Euphorbiaceae). MATERIALS AND METHODS: Antimicrobial activity of flavonoids (free and bound) of Euphorbia hirta L. was determined by disc diffusion assay against four bacteria (Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis, and Staphylococcus aureus) and four fungi (Aspergillus flavus, Aspergillus niger, Trichophyton mentagrophytes, and Candida albicans). Minimum inhibitory concentration (MIC) of the extract was evaluated through micro broth dilution method, while minimum bactericidal/fungicidal concentration was determined by subculturing the relevant samples. Total activity (TA) of extracts against each sensitive pathogen was also evaluated. RESULTS: Out of fungi; A. flavus, A. niger, and T. mentagrophytes were found to be resistant, against which none of the tested extracts showed activity. Bound flavonoids extract of root showed best activity against C. albicans (inhibition zone (IZ) 27.66, MIC 0.039, minimum fungicidal concentration (MFC) 0.039). TA of free flavonoid extract of root was found to be the same for P. mirabilis and S. aureus (192.30 ml/g). Two flavonoids quercetin and kaempferol were identified in the bound flavonoids of stem extract which showed activity against all the microorganisms. CONCLUSION: Results of the present investigation indicate that E. hirta has good antimicrobial activity with low range of MIC, hence can be exploited for future plant-based antimicrobial drugs. Medknow Publications & Media Pvt Ltd 2013 /pmc/articles/PMC3783663/ /pubmed/24083146 http://dx.doi.org/10.4103/2229-516X.117082 Text en Copyright: © International Journal of Applied and Basic Medical Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Singh, Geeta
Kumar, Padma
Phytochemical study and screening for antimicrobial activity of flavonoids of Euphorbia hirta
title Phytochemical study and screening for antimicrobial activity of flavonoids of Euphorbia hirta
title_full Phytochemical study and screening for antimicrobial activity of flavonoids of Euphorbia hirta
title_fullStr Phytochemical study and screening for antimicrobial activity of flavonoids of Euphorbia hirta
title_full_unstemmed Phytochemical study and screening for antimicrobial activity of flavonoids of Euphorbia hirta
title_short Phytochemical study and screening for antimicrobial activity of flavonoids of Euphorbia hirta
title_sort phytochemical study and screening for antimicrobial activity of flavonoids of euphorbia hirta
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3783663/
https://www.ncbi.nlm.nih.gov/pubmed/24083146
http://dx.doi.org/10.4103/2229-516X.117082
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