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Phytochemical study and screening for antimicrobial activity of flavonoids of Euphorbia hirta
OBJECTIVE: This study aims to phytochemical and antimicrobial study of Euphorbia hirta Euphorbiaceae). MATERIALS AND METHODS: Antimicrobial activity of flavonoids (free and bound) of Euphorbia hirta L. was determined by disc diffusion assay against four bacteria (Escherichia coli, Pseudomonas aerugi...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2013
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3783663/ https://www.ncbi.nlm.nih.gov/pubmed/24083146 http://dx.doi.org/10.4103/2229-516X.117082 |
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author | Singh, Geeta Kumar, Padma |
author_facet | Singh, Geeta Kumar, Padma |
author_sort | Singh, Geeta |
collection | PubMed |
description | OBJECTIVE: This study aims to phytochemical and antimicrobial study of Euphorbia hirta Euphorbiaceae). MATERIALS AND METHODS: Antimicrobial activity of flavonoids (free and bound) of Euphorbia hirta L. was determined by disc diffusion assay against four bacteria (Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis, and Staphylococcus aureus) and four fungi (Aspergillus flavus, Aspergillus niger, Trichophyton mentagrophytes, and Candida albicans). Minimum inhibitory concentration (MIC) of the extract was evaluated through micro broth dilution method, while minimum bactericidal/fungicidal concentration was determined by subculturing the relevant samples. Total activity (TA) of extracts against each sensitive pathogen was also evaluated. RESULTS: Out of fungi; A. flavus, A. niger, and T. mentagrophytes were found to be resistant, against which none of the tested extracts showed activity. Bound flavonoids extract of root showed best activity against C. albicans (inhibition zone (IZ) 27.66, MIC 0.039, minimum fungicidal concentration (MFC) 0.039). TA of free flavonoid extract of root was found to be the same for P. mirabilis and S. aureus (192.30 ml/g). Two flavonoids quercetin and kaempferol were identified in the bound flavonoids of stem extract which showed activity against all the microorganisms. CONCLUSION: Results of the present investigation indicate that E. hirta has good antimicrobial activity with low range of MIC, hence can be exploited for future plant-based antimicrobial drugs. |
format | Online Article Text |
id | pubmed-3783663 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-37836632013-09-30 Phytochemical study and screening for antimicrobial activity of flavonoids of Euphorbia hirta Singh, Geeta Kumar, Padma Int J Appl Basic Med Res Original Article OBJECTIVE: This study aims to phytochemical and antimicrobial study of Euphorbia hirta Euphorbiaceae). MATERIALS AND METHODS: Antimicrobial activity of flavonoids (free and bound) of Euphorbia hirta L. was determined by disc diffusion assay against four bacteria (Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis, and Staphylococcus aureus) and four fungi (Aspergillus flavus, Aspergillus niger, Trichophyton mentagrophytes, and Candida albicans). Minimum inhibitory concentration (MIC) of the extract was evaluated through micro broth dilution method, while minimum bactericidal/fungicidal concentration was determined by subculturing the relevant samples. Total activity (TA) of extracts against each sensitive pathogen was also evaluated. RESULTS: Out of fungi; A. flavus, A. niger, and T. mentagrophytes were found to be resistant, against which none of the tested extracts showed activity. Bound flavonoids extract of root showed best activity against C. albicans (inhibition zone (IZ) 27.66, MIC 0.039, minimum fungicidal concentration (MFC) 0.039). TA of free flavonoid extract of root was found to be the same for P. mirabilis and S. aureus (192.30 ml/g). Two flavonoids quercetin and kaempferol were identified in the bound flavonoids of stem extract which showed activity against all the microorganisms. CONCLUSION: Results of the present investigation indicate that E. hirta has good antimicrobial activity with low range of MIC, hence can be exploited for future plant-based antimicrobial drugs. Medknow Publications & Media Pvt Ltd 2013 /pmc/articles/PMC3783663/ /pubmed/24083146 http://dx.doi.org/10.4103/2229-516X.117082 Text en Copyright: © International Journal of Applied and Basic Medical Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Singh, Geeta Kumar, Padma Phytochemical study and screening for antimicrobial activity of flavonoids of Euphorbia hirta |
title | Phytochemical study and screening for antimicrobial activity of flavonoids of Euphorbia hirta |
title_full | Phytochemical study and screening for antimicrobial activity of flavonoids of Euphorbia hirta |
title_fullStr | Phytochemical study and screening for antimicrobial activity of flavonoids of Euphorbia hirta |
title_full_unstemmed | Phytochemical study and screening for antimicrobial activity of flavonoids of Euphorbia hirta |
title_short | Phytochemical study and screening for antimicrobial activity of flavonoids of Euphorbia hirta |
title_sort | phytochemical study and screening for antimicrobial activity of flavonoids of euphorbia hirta |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3783663/ https://www.ncbi.nlm.nih.gov/pubmed/24083146 http://dx.doi.org/10.4103/2229-516X.117082 |
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