Diversity of endophytic bacteria in medicinally important Nepenthes species

BACKGROUND: Nepenthes species are used in traditional medicines to treat various health ailments. However, we do not know which types of endophytic bacteria (EB) are associated with Nepenthes spp. OBJECTIVE: The objective of this study was to isolate and to identify EB associated with Nepenthes spp....

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Detalles Bibliográficos
Autores principales: Bhore, Subhash J., Komathi, Vijayan, Kandasamy, Kodi I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2013
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3783794/
https://www.ncbi.nlm.nih.gov/pubmed/24082746
http://dx.doi.org/10.4103/0976-9668.117022
Descripción
Sumario:BACKGROUND: Nepenthes species are used in traditional medicines to treat various health ailments. However, we do not know which types of endophytic bacteria (EB) are associated with Nepenthes spp. OBJECTIVE: The objective of this study was to isolate and to identify EB associated with Nepenthes spp. MATERIALS AND METHODS: Surface-sterilized leaf and stem tissues from nine Nepenthes spp. collected from Peninsular Malaysia were used to isolate EB. Isolates were identified using the polymerase chain reaction-amplified 16S ribosomal DNA (rDNA) sequence similarity based method. RESULTS: Cultivable, 96 isolates were analyzed; and the 16S rDNA sequences analysis suggest that diverse bacterial species are associated with Nepenthes spp. Majority (55.2%) of the isolates were from Bacillus genus, and Bacillus cereus was the most dominant (14.6%) among isolates. CONCLUSION: Nepenthes spp. do harbor a wide array of cultivable endophytic bacteria.

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