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Regulation of paclitaxel-induced programmed cell death by autophagic induction: A model for cervical cancer

OBJECTIVE: Autophagy plays a vital role in homeostasis by combining organelles and cellular proteins with lysosome under starvation conditions. In addition, autophagy provides tumor cells with a source of energy. Continued autophagy will induce cells death. Here we aim to see if autophagic induction...

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Autores principales: Chi, Eun Young, Viriyapak, Boonlert, Kwack, Hyun Sung, Lee, Yoon Kyung, Kim, Sang Il, Lee, Keun Ho, Park, Tae Churl
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Obstetrics and Gynecology; Korean Society of Contraception and Reproductive Health; Korean Society of Gynecologic Endocrinology; Korean Society of Gynecologic Endoscopy and Minimal Invasive Surgery; Korean Society of Maternal Fetal Medicine; Korean Society of Ultrasound in Obstetrics and Gynecology 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3784092/
https://www.ncbi.nlm.nih.gov/pubmed/24327986
http://dx.doi.org/10.5468/OGS.2013.56.2.84
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author Chi, Eun Young
Viriyapak, Boonlert
Kwack, Hyun Sung
Lee, Yoon Kyung
Kim, Sang Il
Lee, Keun Ho
Park, Tae Churl
author_facet Chi, Eun Young
Viriyapak, Boonlert
Kwack, Hyun Sung
Lee, Yoon Kyung
Kim, Sang Il
Lee, Keun Ho
Park, Tae Churl
author_sort Chi, Eun Young
collection PubMed
description OBJECTIVE: Autophagy plays a vital role in homeostasis by combining organelles and cellular proteins with lysosome under starvation conditions. In addition, autophagy provides tumor cells with a source of energy. Continued autophagy will induce cells death. Here we aim to see if autophagic induction has an effect on conventional chemotherapeutic agents. METHODS: Rapamycin, or mammalian target of rapamycin and paclitaxel, apoptosis-inducing agents were used autophagy in HeLa cervical cancer cells. RESULTS: Growth inhibition of cells was not observed after the application of 0, 10, 20 nM of paclitaxel with or without rapamycin. Using a 5 nM concentration of paclitaxel, rapamycin administration inhibited cell growth significantly compared to no treatment. This implies the synergic antitumor effect of paclitaxel and rapamycin. Paclitaxel itself did not show any autophagic effect on cells but did show cell apoptosis by flow cytometry. Light chain 3, a microtubule-associated protein, which reflect autophagy, was increased with 5 nM of paclitaxel after pretreatment with 10 nM of rapamycin. CONCLUSION: These findings suggest that the autophagic inducer, rapamycin, can potentiate autophagic cell death when added as an apoptosis-inducing chemotherapeutic agent. In conclusion, the control of autophagy may be a future target for chemotherapy.
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spelling pubmed-37840922013-12-10 Regulation of paclitaxel-induced programmed cell death by autophagic induction: A model for cervical cancer Chi, Eun Young Viriyapak, Boonlert Kwack, Hyun Sung Lee, Yoon Kyung Kim, Sang Il Lee, Keun Ho Park, Tae Churl Obstet Gynecol Sci Original Article OBJECTIVE: Autophagy plays a vital role in homeostasis by combining organelles and cellular proteins with lysosome under starvation conditions. In addition, autophagy provides tumor cells with a source of energy. Continued autophagy will induce cells death. Here we aim to see if autophagic induction has an effect on conventional chemotherapeutic agents. METHODS: Rapamycin, or mammalian target of rapamycin and paclitaxel, apoptosis-inducing agents were used autophagy in HeLa cervical cancer cells. RESULTS: Growth inhibition of cells was not observed after the application of 0, 10, 20 nM of paclitaxel with or without rapamycin. Using a 5 nM concentration of paclitaxel, rapamycin administration inhibited cell growth significantly compared to no treatment. This implies the synergic antitumor effect of paclitaxel and rapamycin. Paclitaxel itself did not show any autophagic effect on cells but did show cell apoptosis by flow cytometry. Light chain 3, a microtubule-associated protein, which reflect autophagy, was increased with 5 nM of paclitaxel after pretreatment with 10 nM of rapamycin. CONCLUSION: These findings suggest that the autophagic inducer, rapamycin, can potentiate autophagic cell death when added as an apoptosis-inducing chemotherapeutic agent. In conclusion, the control of autophagy may be a future target for chemotherapy. Korean Society of Obstetrics and Gynecology; Korean Society of Contraception and Reproductive Health; Korean Society of Gynecologic Endocrinology; Korean Society of Gynecologic Endoscopy and Minimal Invasive Surgery; Korean Society of Maternal Fetal Medicine; Korean Society of Ultrasound in Obstetrics and Gynecology 2013-03 2013-03-12 /pmc/articles/PMC3784092/ /pubmed/24327986 http://dx.doi.org/10.5468/OGS.2013.56.2.84 Text en Copyright © 2013 Korean Society of Obstetrics and Gynecology http://creativecommons.org/licenses/by-nc/3.0/ Articles published in Obstet Gynecol Sci are open-access, distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Chi, Eun Young
Viriyapak, Boonlert
Kwack, Hyun Sung
Lee, Yoon Kyung
Kim, Sang Il
Lee, Keun Ho
Park, Tae Churl
Regulation of paclitaxel-induced programmed cell death by autophagic induction: A model for cervical cancer
title Regulation of paclitaxel-induced programmed cell death by autophagic induction: A model for cervical cancer
title_full Regulation of paclitaxel-induced programmed cell death by autophagic induction: A model for cervical cancer
title_fullStr Regulation of paclitaxel-induced programmed cell death by autophagic induction: A model for cervical cancer
title_full_unstemmed Regulation of paclitaxel-induced programmed cell death by autophagic induction: A model for cervical cancer
title_short Regulation of paclitaxel-induced programmed cell death by autophagic induction: A model for cervical cancer
title_sort regulation of paclitaxel-induced programmed cell death by autophagic induction: a model for cervical cancer
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3784092/
https://www.ncbi.nlm.nih.gov/pubmed/24327986
http://dx.doi.org/10.5468/OGS.2013.56.2.84
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