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An Immunocytochemical Study of Interchromatin Granule Clusters in Early Mouse Embryos

Interchromatin granule clusters (IGCs) are universal nuclear domains. Their molecular composition and functions were studied in detail in somatic cells. Here, we studied IGCs in the nuclei of early mouse embryos during zygotic gene activation (ZGA). We found that the size of IGCs gradually increases...

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Detalles Bibliográficos
Autores principales: Bogolyubova, Irina, Bogolyubov, Dmitry
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3784238/
https://www.ncbi.nlm.nih.gov/pubmed/24106723
http://dx.doi.org/10.1155/2013/931564
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author Bogolyubova, Irina
Bogolyubov, Dmitry
author_facet Bogolyubova, Irina
Bogolyubov, Dmitry
author_sort Bogolyubova, Irina
collection PubMed
description Interchromatin granule clusters (IGCs) are universal nuclear domains. Their molecular composition and functions were studied in detail in somatic cells. Here, we studied IGCs in the nuclei of early mouse embryos during zygotic gene activation (ZGA). We found that the size of IGCs gradually increases during realization of ZGA events. Using immunocytochemical approaches, we showed that the molecular composition of IGCs is also modified in mouse embryos. The hyperphosphorylated form of RNA polymerase II and the transcription factor TFIID have been revealed in IGCs before the end of ZGA. Association of these factors with IGCs became more noticeable during ZGA realization. Our data suggest that IGCs in early mouse embryos have some functional peculiarities connected most probably with IGC formation de novo. We believe that IGCs in early mouse embryos not only are storage sites of splicing factors but also may be involved in mRNA metabolism and represent the multifunctional nuclear domains.
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spelling pubmed-37842382013-10-08 An Immunocytochemical Study of Interchromatin Granule Clusters in Early Mouse Embryos Bogolyubova, Irina Bogolyubov, Dmitry Biomed Res Int Research Article Interchromatin granule clusters (IGCs) are universal nuclear domains. Their molecular composition and functions were studied in detail in somatic cells. Here, we studied IGCs in the nuclei of early mouse embryos during zygotic gene activation (ZGA). We found that the size of IGCs gradually increases during realization of ZGA events. Using immunocytochemical approaches, we showed that the molecular composition of IGCs is also modified in mouse embryos. The hyperphosphorylated form of RNA polymerase II and the transcription factor TFIID have been revealed in IGCs before the end of ZGA. Association of these factors with IGCs became more noticeable during ZGA realization. Our data suggest that IGCs in early mouse embryos have some functional peculiarities connected most probably with IGC formation de novo. We believe that IGCs in early mouse embryos not only are storage sites of splicing factors but also may be involved in mRNA metabolism and represent the multifunctional nuclear domains. Hindawi Publishing Corporation 2013 2013-09-11 /pmc/articles/PMC3784238/ /pubmed/24106723 http://dx.doi.org/10.1155/2013/931564 Text en Copyright © 2013 I. Bogolyubova and D. Bogolyubov. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Bogolyubova, Irina
Bogolyubov, Dmitry
An Immunocytochemical Study of Interchromatin Granule Clusters in Early Mouse Embryos
title An Immunocytochemical Study of Interchromatin Granule Clusters in Early Mouse Embryos
title_full An Immunocytochemical Study of Interchromatin Granule Clusters in Early Mouse Embryos
title_fullStr An Immunocytochemical Study of Interchromatin Granule Clusters in Early Mouse Embryos
title_full_unstemmed An Immunocytochemical Study of Interchromatin Granule Clusters in Early Mouse Embryos
title_short An Immunocytochemical Study of Interchromatin Granule Clusters in Early Mouse Embryos
title_sort immunocytochemical study of interchromatin granule clusters in early mouse embryos
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3784238/
https://www.ncbi.nlm.nih.gov/pubmed/24106723
http://dx.doi.org/10.1155/2013/931564
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