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Protein kinase C activation decreases peripheral actin network density and increases central nonmuscle myosin II contractility in neuronal growth cones

Protein kinase C (PKC) can dramatically alter cell structure and motility via effects on actin filament networks. In neurons, PKC activation has been implicated in repulsive guidance responses and inhibition of axon regeneration; however, the cytoskeletal mechanisms underlying these effects are not...

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Autores principales: Yang, Qing, Zhang, Xiao-Feng, Van Goor, David, Dunn, Ashleigh P., Hyland, Callen, Medeiros, Nelson, Forscher, Paul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3784383/
https://www.ncbi.nlm.nih.gov/pubmed/23966465
http://dx.doi.org/10.1091/mbc.E13-05-0289
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author Yang, Qing
Zhang, Xiao-Feng
Van Goor, David
Dunn, Ashleigh P.
Hyland, Callen
Medeiros, Nelson
Forscher, Paul
author_facet Yang, Qing
Zhang, Xiao-Feng
Van Goor, David
Dunn, Ashleigh P.
Hyland, Callen
Medeiros, Nelson
Forscher, Paul
author_sort Yang, Qing
collection PubMed
description Protein kinase C (PKC) can dramatically alter cell structure and motility via effects on actin filament networks. In neurons, PKC activation has been implicated in repulsive guidance responses and inhibition of axon regeneration; however, the cytoskeletal mechanisms underlying these effects are not well understood. Here we investigate the acute effects of PKC activation on actin network structure and dynamics in large Aplysia neuronal growth cones. We provide evidence of a novel two-tiered mechanism of PKC action: 1) PKC activity enhances myosin II regulatory light chain phosphorylation and C-kinase–potentiated protein phosphatase inhibitor phosphorylation. These effects are correlated with increased contractility in the central cytoplasmic domain. 2) PKC activation results in significant reduction of P-domain actin network density accompanied by Arp2/3 complex delocalization from the leading edge and increased rates of retrograde actin network flow. Our results show that PKC activation strongly affects both actin polymerization and myosin II contractility. This synergistic mode of action is relevant to understanding the pleiotropic reported effects of PKC on neuronal growth and regeneration.
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spelling pubmed-37843832013-12-16 Protein kinase C activation decreases peripheral actin network density and increases central nonmuscle myosin II contractility in neuronal growth cones Yang, Qing Zhang, Xiao-Feng Van Goor, David Dunn, Ashleigh P. Hyland, Callen Medeiros, Nelson Forscher, Paul Mol Biol Cell Articles Protein kinase C (PKC) can dramatically alter cell structure and motility via effects on actin filament networks. In neurons, PKC activation has been implicated in repulsive guidance responses and inhibition of axon regeneration; however, the cytoskeletal mechanisms underlying these effects are not well understood. Here we investigate the acute effects of PKC activation on actin network structure and dynamics in large Aplysia neuronal growth cones. We provide evidence of a novel two-tiered mechanism of PKC action: 1) PKC activity enhances myosin II regulatory light chain phosphorylation and C-kinase–potentiated protein phosphatase inhibitor phosphorylation. These effects are correlated with increased contractility in the central cytoplasmic domain. 2) PKC activation results in significant reduction of P-domain actin network density accompanied by Arp2/3 complex delocalization from the leading edge and increased rates of retrograde actin network flow. Our results show that PKC activation strongly affects both actin polymerization and myosin II contractility. This synergistic mode of action is relevant to understanding the pleiotropic reported effects of PKC on neuronal growth and regeneration. The American Society for Cell Biology 2013-10-01 /pmc/articles/PMC3784383/ /pubmed/23966465 http://dx.doi.org/10.1091/mbc.E13-05-0289 Text en © 2013 Yang et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Yang, Qing
Zhang, Xiao-Feng
Van Goor, David
Dunn, Ashleigh P.
Hyland, Callen
Medeiros, Nelson
Forscher, Paul
Protein kinase C activation decreases peripheral actin network density and increases central nonmuscle myosin II contractility in neuronal growth cones
title Protein kinase C activation decreases peripheral actin network density and increases central nonmuscle myosin II contractility in neuronal growth cones
title_full Protein kinase C activation decreases peripheral actin network density and increases central nonmuscle myosin II contractility in neuronal growth cones
title_fullStr Protein kinase C activation decreases peripheral actin network density and increases central nonmuscle myosin II contractility in neuronal growth cones
title_full_unstemmed Protein kinase C activation decreases peripheral actin network density and increases central nonmuscle myosin II contractility in neuronal growth cones
title_short Protein kinase C activation decreases peripheral actin network density and increases central nonmuscle myosin II contractility in neuronal growth cones
title_sort protein kinase c activation decreases peripheral actin network density and increases central nonmuscle myosin ii contractility in neuronal growth cones
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3784383/
https://www.ncbi.nlm.nih.gov/pubmed/23966465
http://dx.doi.org/10.1091/mbc.E13-05-0289
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