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Barcoding T Cell Calcium Response Diversity with Methods for Automated and Accurate Analysis of Cell Signals (MAAACS)

We introduce a series of experimental procedures enabling sensitive calcium monitoring in T cell populations by confocal video-microscopy. Tracking and post-acquisition analysis was performed using Methods for Automated and Accurate Analysis of Cell Signals (MAAACS), a fully customized program that...

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Detalles Bibliográficos
Autores principales: Salles, Audrey, Billaudeau, Cyrille, Sergé, Arnauld, Bernard, Anne-Marie, Phélipot, Marie-Claire, Bertaux, Nicolas, Fallet, Mathieu, Grenot, Pierre, Marguet, Didier, He, Hai-Tao, Hamon, Yannick
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3784497/
https://www.ncbi.nlm.nih.gov/pubmed/24086124
http://dx.doi.org/10.1371/journal.pcbi.1003245
Descripción
Sumario:We introduce a series of experimental procedures enabling sensitive calcium monitoring in T cell populations by confocal video-microscopy. Tracking and post-acquisition analysis was performed using Methods for Automated and Accurate Analysis of Cell Signals (MAAACS), a fully customized program that associates a high throughput tracking algorithm, an intuitive reconnection routine and a statistical platform to provide, at a glance, the calcium barcode of a population of individual T-cells. Combined with a sensitive calcium probe, this method allowed us to unravel the heterogeneity in shape and intensity of the calcium response in T cell populations and especially in naive T cells, which display intracellular calcium oscillations upon stimulation by antigen presenting cells.