Complete Genome Sequence of a Cytomegalovirus Towne-BAC (Bacterial Artificial Chromosome) Isolate Maintained in Escherichia coli for 10 Years and Then Serially Passaged in Human Fibroblasts

Here, we present the complete genome sequence of a cytomegalovirus, the Towne-BAC (bacterial artificial chromosome) isolate, which was maintained in bacterial cells for 10 years and then serially passaged in human fibroblasts for 10 passages. A total of 132 nucleotide differences were discovered in...

Descripción completa

Detalles Bibliográficos
Autores principales: Brechtel, Teal, Tyner, Molly, Tandon, Ritesh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2013
Materias:
Viruses
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3784777/
https://www.ncbi.nlm.nih.gov/pubmed/24072857
http://dx.doi.org/10.1128/genomeA.00693-13
_version_ 1782477593890521088
author Brechtel, Teal
Tyner, Molly
Tandon, Ritesh
author_facet Brechtel, Teal
Tyner, Molly
Tandon, Ritesh
author_sort Brechtel, Teal
collection PubMed
description Here, we present the complete genome sequence of a cytomegalovirus, the Towne-BAC (bacterial artificial chromosome) isolate, which was maintained in bacterial cells for 10 years and then serially passaged in human fibroblasts for 10 passages. A total of 132 nucleotide differences were discovered in the Towne sequence compared to the reference sequence (GenBank accession no. AC146851).
format Online
Article
Text
id pubmed-3784777
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher American Society for Microbiology
record_format MEDLINE/PubMed
spelling pubmed-37847772013-09-30 Complete Genome Sequence of a Cytomegalovirus Towne-BAC (Bacterial Artificial Chromosome) Isolate Maintained in Escherichia coli for 10 Years and Then Serially Passaged in Human Fibroblasts Brechtel, Teal Tyner, Molly Tandon, Ritesh Genome Announc Viruses Here, we present the complete genome sequence of a cytomegalovirus, the Towne-BAC (bacterial artificial chromosome) isolate, which was maintained in bacterial cells for 10 years and then serially passaged in human fibroblasts for 10 passages. A total of 132 nucleotide differences were discovered in the Towne sequence compared to the reference sequence (GenBank accession no. AC146851). American Society for Microbiology 2013-09-26 /pmc/articles/PMC3784777/ /pubmed/24072857 http://dx.doi.org/10.1128/genomeA.00693-13 Text en Copyright © 2013 Brechtel et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license (http://creativecommons.org/licenses/by/3.0/) .
spellingShingle Viruses
Brechtel, Teal
Tyner, Molly
Tandon, Ritesh
Complete Genome Sequence of a Cytomegalovirus Towne-BAC (Bacterial Artificial Chromosome) Isolate Maintained in Escherichia coli for 10 Years and Then Serially Passaged in Human Fibroblasts
title Complete Genome Sequence of a Cytomegalovirus Towne-BAC (Bacterial Artificial Chromosome) Isolate Maintained in Escherichia coli for 10 Years and Then Serially Passaged in Human Fibroblasts
title_full Complete Genome Sequence of a Cytomegalovirus Towne-BAC (Bacterial Artificial Chromosome) Isolate Maintained in Escherichia coli for 10 Years and Then Serially Passaged in Human Fibroblasts
title_fullStr Complete Genome Sequence of a Cytomegalovirus Towne-BAC (Bacterial Artificial Chromosome) Isolate Maintained in Escherichia coli for 10 Years and Then Serially Passaged in Human Fibroblasts
title_full_unstemmed Complete Genome Sequence of a Cytomegalovirus Towne-BAC (Bacterial Artificial Chromosome) Isolate Maintained in Escherichia coli for 10 Years and Then Serially Passaged in Human Fibroblasts
title_short Complete Genome Sequence of a Cytomegalovirus Towne-BAC (Bacterial Artificial Chromosome) Isolate Maintained in Escherichia coli for 10 Years and Then Serially Passaged in Human Fibroblasts
title_sort complete genome sequence of a cytomegalovirus towne-bac (bacterial artificial chromosome) isolate maintained in escherichia coli for 10 years and then serially passaged in human fibroblasts
topic Viruses
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3784777/
https://www.ncbi.nlm.nih.gov/pubmed/24072857
http://dx.doi.org/10.1128/genomeA.00693-13
work_keys_str_mv AT brechtelteal completegenomesequenceofacytomegalovirustownebacbacterialartificialchromosomeisolatemaintainedinescherichiacolifor10yearsandthenseriallypassagedinhumanfibroblasts
AT tynermolly completegenomesequenceofacytomegalovirustownebacbacterialartificialchromosomeisolatemaintainedinescherichiacolifor10yearsandthenseriallypassagedinhumanfibroblasts
AT tandonritesh completegenomesequenceofacytomegalovirustownebacbacterialartificialchromosomeisolatemaintainedinescherichiacolifor10yearsandthenseriallypassagedinhumanfibroblasts

Ejemplares similares