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Chromosome position at the spindle equator is regulated by chromokinesin and a bipolar microtubule array
The chromosome alignment is mediated by polar ejection and poleward forces acting on the chromosome arm and kinetochores, respectively. Although components of the motile machinery such as chromokinesin have been characterized, their dynamics within the spindle is poorly understood. Here we show that...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3786301/ https://www.ncbi.nlm.nih.gov/pubmed/24077015 http://dx.doi.org/10.1038/srep02808 |
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author | Takagi, Jun Itabashi, Takeshi Suzuki, Kazuya Ishiwata, Shin'ichi |
author_facet | Takagi, Jun Itabashi, Takeshi Suzuki, Kazuya Ishiwata, Shin'ichi |
author_sort | Takagi, Jun |
collection | PubMed |
description | The chromosome alignment is mediated by polar ejection and poleward forces acting on the chromosome arm and kinetochores, respectively. Although components of the motile machinery such as chromokinesin have been characterized, their dynamics within the spindle is poorly understood. Here we show that a quantum dot (Qdot) binding up to four Xenopus chromokinesin (Xkid) molecules behaved like a nanosize chromosome arm in the meiotic spindle, which is self-organized in cytoplasmic egg extracts. Xkid-Qdots travelled long distances along microtubules by changing several tracks, resulting in their accumulation toward and distribution around the metaphase plate. The analysis indicated that the direction of motion and velocity depend on the distribution of microtubule polarity within the spindle. Thus, this mechanism is governed by chromokinesin motors, which is dependent on symmetrical microtubule orientation that may allow chromosomes to maintain their position around the spindle equator until correct microtubule–kinetochore attachment is established. |
format | Online Article Text |
id | pubmed-3786301 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-37863012013-09-30 Chromosome position at the spindle equator is regulated by chromokinesin and a bipolar microtubule array Takagi, Jun Itabashi, Takeshi Suzuki, Kazuya Ishiwata, Shin'ichi Sci Rep Article The chromosome alignment is mediated by polar ejection and poleward forces acting on the chromosome arm and kinetochores, respectively. Although components of the motile machinery such as chromokinesin have been characterized, their dynamics within the spindle is poorly understood. Here we show that a quantum dot (Qdot) binding up to four Xenopus chromokinesin (Xkid) molecules behaved like a nanosize chromosome arm in the meiotic spindle, which is self-organized in cytoplasmic egg extracts. Xkid-Qdots travelled long distances along microtubules by changing several tracks, resulting in their accumulation toward and distribution around the metaphase plate. The analysis indicated that the direction of motion and velocity depend on the distribution of microtubule polarity within the spindle. Thus, this mechanism is governed by chromokinesin motors, which is dependent on symmetrical microtubule orientation that may allow chromosomes to maintain their position around the spindle equator until correct microtubule–kinetochore attachment is established. Nature Publishing Group 2013-09-30 /pmc/articles/PMC3786301/ /pubmed/24077015 http://dx.doi.org/10.1038/srep02808 Text en Copyright © 2013, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Article Takagi, Jun Itabashi, Takeshi Suzuki, Kazuya Ishiwata, Shin'ichi Chromosome position at the spindle equator is regulated by chromokinesin and a bipolar microtubule array |
title | Chromosome position at the spindle equator is regulated by chromokinesin and a bipolar microtubule array |
title_full | Chromosome position at the spindle equator is regulated by chromokinesin and a bipolar microtubule array |
title_fullStr | Chromosome position at the spindle equator is regulated by chromokinesin and a bipolar microtubule array |
title_full_unstemmed | Chromosome position at the spindle equator is regulated by chromokinesin and a bipolar microtubule array |
title_short | Chromosome position at the spindle equator is regulated by chromokinesin and a bipolar microtubule array |
title_sort | chromosome position at the spindle equator is regulated by chromokinesin and a bipolar microtubule array |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3786301/ https://www.ncbi.nlm.nih.gov/pubmed/24077015 http://dx.doi.org/10.1038/srep02808 |
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