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Stability Studies of Thiocolchicoside in Bulk and Capsules Using RP-HPTLC/Densitometry
A new stability-indicating reversed-phase high-performance thin-layer chromatographic (RP-HPTLC) method for densitometric analysis of thiocolchicoside was developed and validated. The chromatograms were developed using aluminum plates pre-coated with silica gel 60 RP-18 F(254)S as a stationary phase...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3786465/ https://www.ncbi.nlm.nih.gov/pubmed/24109541 http://dx.doi.org/10.1155/2013/142628 |
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author | Rajput, Dnyansing K. Shirkhedkar, Atul A. Rajput, Jyoti K. Patel, Harun M. Surana, Sanjay J. |
author_facet | Rajput, Dnyansing K. Shirkhedkar, Atul A. Rajput, Jyoti K. Patel, Harun M. Surana, Sanjay J. |
author_sort | Rajput, Dnyansing K. |
collection | PubMed |
description | A new stability-indicating reversed-phase high-performance thin-layer chromatographic (RP-HPTLC) method for densitometric analysis of thiocolchicoside was developed and validated. The chromatograms were developed using aluminum plates pre-coated with silica gel 60 RP-18 F(254)S as a stationary phase and methanol : water (70 : 30 v/v) as a mobile phase. The compact band for thiocolchicoside was observed at R (f) value of 0.60 ± 0.02 at an absorption wavelength of 377 nm. The linear regression data for the calibration plots (r (2) = 0.9984) was found with respect to peak area in the concentration range of 100–600 ng per band. The limit of detection (LOD) and limit of quantification (LOQ) were 9.77 ng and 29.63 ng, respectively. The drug was exposed to acidic and alkaline hydrolysis, oxidation, photo degradation, and dry heat conditions. The peaks of degradation products were well-resolved from the peak of the standard drug with significantly different R (f) values. Statistical analysis proved that the established RP-HPTLC method is reproducible, selective, and accurate for the determination of thiocolchicoside in its formulations. The method can effectively separate the drug from its degradation products, and it can be considered as stability-indicating assay. |
format | Online Article Text |
id | pubmed-3786465 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-37864652013-10-09 Stability Studies of Thiocolchicoside in Bulk and Capsules Using RP-HPTLC/Densitometry Rajput, Dnyansing K. Shirkhedkar, Atul A. Rajput, Jyoti K. Patel, Harun M. Surana, Sanjay J. J Anal Methods Chem Research Article A new stability-indicating reversed-phase high-performance thin-layer chromatographic (RP-HPTLC) method for densitometric analysis of thiocolchicoside was developed and validated. The chromatograms were developed using aluminum plates pre-coated with silica gel 60 RP-18 F(254)S as a stationary phase and methanol : water (70 : 30 v/v) as a mobile phase. The compact band for thiocolchicoside was observed at R (f) value of 0.60 ± 0.02 at an absorption wavelength of 377 nm. The linear regression data for the calibration plots (r (2) = 0.9984) was found with respect to peak area in the concentration range of 100–600 ng per band. The limit of detection (LOD) and limit of quantification (LOQ) were 9.77 ng and 29.63 ng, respectively. The drug was exposed to acidic and alkaline hydrolysis, oxidation, photo degradation, and dry heat conditions. The peaks of degradation products were well-resolved from the peak of the standard drug with significantly different R (f) values. Statistical analysis proved that the established RP-HPTLC method is reproducible, selective, and accurate for the determination of thiocolchicoside in its formulations. The method can effectively separate the drug from its degradation products, and it can be considered as stability-indicating assay. Hindawi Publishing Corporation 2013 2013-09-11 /pmc/articles/PMC3786465/ /pubmed/24109541 http://dx.doi.org/10.1155/2013/142628 Text en Copyright © 2013 Dnyansing K. Rajput et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Rajput, Dnyansing K. Shirkhedkar, Atul A. Rajput, Jyoti K. Patel, Harun M. Surana, Sanjay J. Stability Studies of Thiocolchicoside in Bulk and Capsules Using RP-HPTLC/Densitometry |
title | Stability Studies of Thiocolchicoside in Bulk and Capsules Using RP-HPTLC/Densitometry |
title_full | Stability Studies of Thiocolchicoside in Bulk and Capsules Using RP-HPTLC/Densitometry |
title_fullStr | Stability Studies of Thiocolchicoside in Bulk and Capsules Using RP-HPTLC/Densitometry |
title_full_unstemmed | Stability Studies of Thiocolchicoside in Bulk and Capsules Using RP-HPTLC/Densitometry |
title_short | Stability Studies of Thiocolchicoside in Bulk and Capsules Using RP-HPTLC/Densitometry |
title_sort | stability studies of thiocolchicoside in bulk and capsules using rp-hptlc/densitometry |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3786465/ https://www.ncbi.nlm.nih.gov/pubmed/24109541 http://dx.doi.org/10.1155/2013/142628 |
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