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The Use of Porous Scaffold as a Tumor Model
Background. Human cancer is a three-dimensional (3D) structure consisting of neighboring cells, extracellular matrix, and blood vessels. It is therefore critical to mimic the cancer cells and their surrounding environment during in vitro study. Our aim was to establish a 3D cancer model using a synt...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3786466/ https://www.ncbi.nlm.nih.gov/pubmed/24101930 http://dx.doi.org/10.1155/2013/396056 |
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author | Zhang, Mei Boughton, Philip Rose, Barbara Lee, C. Soon Hong, Angela M. |
author_facet | Zhang, Mei Boughton, Philip Rose, Barbara Lee, C. Soon Hong, Angela M. |
author_sort | Zhang, Mei |
collection | PubMed |
description | Background. Human cancer is a three-dimensional (3D) structure consisting of neighboring cells, extracellular matrix, and blood vessels. It is therefore critical to mimic the cancer cells and their surrounding environment during in vitro study. Our aim was to establish a 3D cancer model using a synthetic composite scaffold. Methods. High-density low-volume seeding was used to promote attachment of a non-small-cell lung cancer cell line (NCI-H460) to scaffolds. Growth patterns in 3D culture were compared with those of monolayers. Immunohistochemistry was conducted to compare the expression of Ki67, CD44, and carbonic anhydrase IX. Results. NCI-H460 readily attached to the scaffold without surface pretreatment at a rate of 35% from a load of 1.5 × 10(6) cells. Most cells grew vertically to form clumps along the surface of the scaffold, and cell morphology resembled tissue origin; 2D cultures exhibited characteristics of adherent epithelial cancer cell lines. Expression patterns of Ki67, CD44, and CA IX varied markedly between 3D and monolayer cultures. Conclusions. The behavior of cancer cells in our 3D model is similar to tumor growth in vivo. This model will provide the basis for future study using 3D cancer culture. |
format | Online Article Text |
id | pubmed-3786466 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-37864662013-10-07 The Use of Porous Scaffold as a Tumor Model Zhang, Mei Boughton, Philip Rose, Barbara Lee, C. Soon Hong, Angela M. Int J Biomater Research Article Background. Human cancer is a three-dimensional (3D) structure consisting of neighboring cells, extracellular matrix, and blood vessels. It is therefore critical to mimic the cancer cells and their surrounding environment during in vitro study. Our aim was to establish a 3D cancer model using a synthetic composite scaffold. Methods. High-density low-volume seeding was used to promote attachment of a non-small-cell lung cancer cell line (NCI-H460) to scaffolds. Growth patterns in 3D culture were compared with those of monolayers. Immunohistochemistry was conducted to compare the expression of Ki67, CD44, and carbonic anhydrase IX. Results. NCI-H460 readily attached to the scaffold without surface pretreatment at a rate of 35% from a load of 1.5 × 10(6) cells. Most cells grew vertically to form clumps along the surface of the scaffold, and cell morphology resembled tissue origin; 2D cultures exhibited characteristics of adherent epithelial cancer cell lines. Expression patterns of Ki67, CD44, and CA IX varied markedly between 3D and monolayer cultures. Conclusions. The behavior of cancer cells in our 3D model is similar to tumor growth in vivo. This model will provide the basis for future study using 3D cancer culture. Hindawi Publishing Corporation 2013 2013-09-11 /pmc/articles/PMC3786466/ /pubmed/24101930 http://dx.doi.org/10.1155/2013/396056 Text en Copyright © 2013 Mei Zhang et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Zhang, Mei Boughton, Philip Rose, Barbara Lee, C. Soon Hong, Angela M. The Use of Porous Scaffold as a Tumor Model |
title | The Use of Porous Scaffold as a Tumor Model |
title_full | The Use of Porous Scaffold as a Tumor Model |
title_fullStr | The Use of Porous Scaffold as a Tumor Model |
title_full_unstemmed | The Use of Porous Scaffold as a Tumor Model |
title_short | The Use of Porous Scaffold as a Tumor Model |
title_sort | use of porous scaffold as a tumor model |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3786466/ https://www.ncbi.nlm.nih.gov/pubmed/24101930 http://dx.doi.org/10.1155/2013/396056 |
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