A base pair resolution map of nucleosome positions in yeast

The exact positions of nucleosomes along genomic DNA can influence many aspects of chromosome function, yet existing methods for mapping nucleosomes do not provide the necessary single base pair accuracy to determine these positions. Here we develop and apply a new approach for direct mapping of nuc...

Descripción completa

Detalles Bibliográficos
Autores principales: Brogaard, Kristin, Xi, Liqun, Wang, Ji-Ping, Widom, Jonathan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3786739/
https://www.ncbi.nlm.nih.gov/pubmed/22722846
http://dx.doi.org/10.1038/nature11142
_version_ 1782477772819529728
author Brogaard, Kristin
Xi, Liqun
Wang, Ji-Ping
Widom, Jonathan
author_facet Brogaard, Kristin
Xi, Liqun
Wang, Ji-Ping
Widom, Jonathan
author_sort Brogaard, Kristin
collection PubMed
description The exact positions of nucleosomes along genomic DNA can influence many aspects of chromosome function, yet existing methods for mapping nucleosomes do not provide the necessary single base pair accuracy to determine these positions. Here we develop and apply a new approach for direct mapping of nucleosome centers based on chemical modification of engineered histones. The resulting map locates nucleosome positions genome-wide in unprecedented detail and accuracy. It reveals novel aspects of the in vivo nucleosome organization that are linked to transcription factor binding, RNA polymerase pausing, and the higher order structure of the chromatin fiber.
format Online
Article
Text
id pubmed-3786739
institution National Center for Biotechnology Information
language English
publishDate 2012
record_format MEDLINE/PubMed
spelling pubmed-37867392013-09-30 A base pair resolution map of nucleosome positions in yeast Brogaard, Kristin Xi, Liqun Wang, Ji-Ping Widom, Jonathan Nature Article The exact positions of nucleosomes along genomic DNA can influence many aspects of chromosome function, yet existing methods for mapping nucleosomes do not provide the necessary single base pair accuracy to determine these positions. Here we develop and apply a new approach for direct mapping of nucleosome centers based on chemical modification of engineered histones. The resulting map locates nucleosome positions genome-wide in unprecedented detail and accuracy. It reveals novel aspects of the in vivo nucleosome organization that are linked to transcription factor binding, RNA polymerase pausing, and the higher order structure of the chromatin fiber. 2012-06-28 /pmc/articles/PMC3786739/ /pubmed/22722846 http://dx.doi.org/10.1038/nature11142 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Brogaard, Kristin
Xi, Liqun
Wang, Ji-Ping
Widom, Jonathan
A base pair resolution map of nucleosome positions in yeast
title A base pair resolution map of nucleosome positions in yeast
title_full A base pair resolution map of nucleosome positions in yeast
title_fullStr A base pair resolution map of nucleosome positions in yeast
title_full_unstemmed A base pair resolution map of nucleosome positions in yeast
title_short A base pair resolution map of nucleosome positions in yeast
title_sort base pair resolution map of nucleosome positions in yeast
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3786739/
https://www.ncbi.nlm.nih.gov/pubmed/22722846
http://dx.doi.org/10.1038/nature11142
work_keys_str_mv AT brogaardkristin abasepairresolutionmapofnucleosomepositionsinyeast
AT xiliqun abasepairresolutionmapofnucleosomepositionsinyeast
AT wangjiping abasepairresolutionmapofnucleosomepositionsinyeast
AT widomjonathan abasepairresolutionmapofnucleosomepositionsinyeast
AT brogaardkristin basepairresolutionmapofnucleosomepositionsinyeast
AT xiliqun basepairresolutionmapofnucleosomepositionsinyeast
AT wangjiping basepairresolutionmapofnucleosomepositionsinyeast
AT widomjonathan basepairresolutionmapofnucleosomepositionsinyeast

Ejemplares similares