Cargando…
The prediction of T- and B-combined epitope and tertiary structure of the Eg95 antigen of Echinococcus granulosus
Echinococcosis, also known as hydatid disease, is a type of zoonotic parasitic disease caused by the Echinococcus larvae infection. The disease is severely harmful to both humans and animals. Research and development of an epitope vaccine is crucial. To determine the dominant epitopes of the Eg95 an...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3786808/ https://www.ncbi.nlm.nih.gov/pubmed/24137242 http://dx.doi.org/10.3892/etm.2013.1187 |
_version_ | 1782477776745398272 |
---|---|
author | MA, XIUMIN ZHOU, XIAOTAO ZHU, YUEJIE LI, YANHUA WANG, HONGYING MAMUTI, WULAMU LI, YUJIAO WEN, HAO DING, JIANBING |
author_facet | MA, XIUMIN ZHOU, XIAOTAO ZHU, YUEJIE LI, YANHUA WANG, HONGYING MAMUTI, WULAMU LI, YUJIAO WEN, HAO DING, JIANBING |
author_sort | MA, XIUMIN |
collection | PubMed |
description | Echinococcosis, also known as hydatid disease, is a type of zoonotic parasitic disease caused by the Echinococcus larvae infection. The disease is severely harmful to both humans and animals. Research and development of an epitope vaccine is crucial. To determine the dominant epitopes of the Eg95 antigen, the tertiary structure and the T- and B-combined epitope of the Eg95 protein for Echinococcus granulosus were predicted and analyzed in the present study. The tertiary structure of the Eg95 protein was predicted using the 3DLigandsite server and RasMol software. The T- and B-combined epitope of the Eg95 antigen was analyzed using the DNAStar (V5.0), IEDB, SYFPEITHI and BIMAS. Tertiary structure prediction results showed that there were potential epitopes in Eg95 antigen. Bioinformatics analysis revealed the T- and B-combined epitopes of Eg95 antigen. Four and six T- and B-combined epitopes induced immune responses in humans and mice. Additionally, four T- and B-combined epitopes induced immune responses in both humans and mice. The tertiary structure and T- and B-combined epitopes of the Eg95 protein were also determined. The results obtained in the present study may be beneficial in the investigation of Eg95 antigenicity and the development of dominant epitope vaccines. |
format | Online Article Text |
id | pubmed-3786808 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-37868082013-10-17 The prediction of T- and B-combined epitope and tertiary structure of the Eg95 antigen of Echinococcus granulosus MA, XIUMIN ZHOU, XIAOTAO ZHU, YUEJIE LI, YANHUA WANG, HONGYING MAMUTI, WULAMU LI, YUJIAO WEN, HAO DING, JIANBING Exp Ther Med Articles Echinococcosis, also known as hydatid disease, is a type of zoonotic parasitic disease caused by the Echinococcus larvae infection. The disease is severely harmful to both humans and animals. Research and development of an epitope vaccine is crucial. To determine the dominant epitopes of the Eg95 antigen, the tertiary structure and the T- and B-combined epitope of the Eg95 protein for Echinococcus granulosus were predicted and analyzed in the present study. The tertiary structure of the Eg95 protein was predicted using the 3DLigandsite server and RasMol software. The T- and B-combined epitope of the Eg95 antigen was analyzed using the DNAStar (V5.0), IEDB, SYFPEITHI and BIMAS. Tertiary structure prediction results showed that there were potential epitopes in Eg95 antigen. Bioinformatics analysis revealed the T- and B-combined epitopes of Eg95 antigen. Four and six T- and B-combined epitopes induced immune responses in humans and mice. Additionally, four T- and B-combined epitopes induced immune responses in both humans and mice. The tertiary structure and T- and B-combined epitopes of the Eg95 protein were also determined. The results obtained in the present study may be beneficial in the investigation of Eg95 antigenicity and the development of dominant epitope vaccines. D.A. Spandidos 2013-09 2013-06-28 /pmc/articles/PMC3786808/ /pubmed/24137242 http://dx.doi.org/10.3892/etm.2013.1187 Text en Copyright © 2013, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Articles MA, XIUMIN ZHOU, XIAOTAO ZHU, YUEJIE LI, YANHUA WANG, HONGYING MAMUTI, WULAMU LI, YUJIAO WEN, HAO DING, JIANBING The prediction of T- and B-combined epitope and tertiary structure of the Eg95 antigen of Echinococcus granulosus |
title | The prediction of T- and B-combined epitope and tertiary structure of the Eg95 antigen of Echinococcus granulosus |
title_full | The prediction of T- and B-combined epitope and tertiary structure of the Eg95 antigen of Echinococcus granulosus |
title_fullStr | The prediction of T- and B-combined epitope and tertiary structure of the Eg95 antigen of Echinococcus granulosus |
title_full_unstemmed | The prediction of T- and B-combined epitope and tertiary structure of the Eg95 antigen of Echinococcus granulosus |
title_short | The prediction of T- and B-combined epitope and tertiary structure of the Eg95 antigen of Echinococcus granulosus |
title_sort | prediction of t- and b-combined epitope and tertiary structure of the eg95 antigen of echinococcus granulosus |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3786808/ https://www.ncbi.nlm.nih.gov/pubmed/24137242 http://dx.doi.org/10.3892/etm.2013.1187 |
work_keys_str_mv | AT maxiumin thepredictionoftandbcombinedepitopeandtertiarystructureoftheeg95antigenofechinococcusgranulosus AT zhouxiaotao thepredictionoftandbcombinedepitopeandtertiarystructureoftheeg95antigenofechinococcusgranulosus AT zhuyuejie thepredictionoftandbcombinedepitopeandtertiarystructureoftheeg95antigenofechinococcusgranulosus AT liyanhua thepredictionoftandbcombinedepitopeandtertiarystructureoftheeg95antigenofechinococcusgranulosus AT wanghongying thepredictionoftandbcombinedepitopeandtertiarystructureoftheeg95antigenofechinococcusgranulosus AT mamutiwulamu thepredictionoftandbcombinedepitopeandtertiarystructureoftheeg95antigenofechinococcusgranulosus AT liyujiao thepredictionoftandbcombinedepitopeandtertiarystructureoftheeg95antigenofechinococcusgranulosus AT wenhao thepredictionoftandbcombinedepitopeandtertiarystructureoftheeg95antigenofechinococcusgranulosus AT dingjianbing thepredictionoftandbcombinedepitopeandtertiarystructureoftheeg95antigenofechinococcusgranulosus AT maxiumin predictionoftandbcombinedepitopeandtertiarystructureoftheeg95antigenofechinococcusgranulosus AT zhouxiaotao predictionoftandbcombinedepitopeandtertiarystructureoftheeg95antigenofechinococcusgranulosus AT zhuyuejie predictionoftandbcombinedepitopeandtertiarystructureoftheeg95antigenofechinococcusgranulosus AT liyanhua predictionoftandbcombinedepitopeandtertiarystructureoftheeg95antigenofechinococcusgranulosus AT wanghongying predictionoftandbcombinedepitopeandtertiarystructureoftheeg95antigenofechinococcusgranulosus AT mamutiwulamu predictionoftandbcombinedepitopeandtertiarystructureoftheeg95antigenofechinococcusgranulosus AT liyujiao predictionoftandbcombinedepitopeandtertiarystructureoftheeg95antigenofechinococcusgranulosus AT wenhao predictionoftandbcombinedepitopeandtertiarystructureoftheeg95antigenofechinococcusgranulosus AT dingjianbing predictionoftandbcombinedepitopeandtertiarystructureoftheeg95antigenofechinococcusgranulosus |