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Homologous Recombination Occurs in Entamoeba and Is Enhanced during Growth Stress and Stage Conversion
Homologous recombination (HR) has not been demonstrated in the parasitic protists Entamoeba histolytica or Entamoeba invadens, as no convenient method is available to measure it. However, HR must exist to ensure genome integrity, and possible genetic exchange, especially during stage conversion from...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3787063/ https://www.ncbi.nlm.nih.gov/pubmed/24098652 http://dx.doi.org/10.1371/journal.pone.0074465 |
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author | Singh, Nishant Bhattacharya, Alok Bhattacharya, Sudha |
author_facet | Singh, Nishant Bhattacharya, Alok Bhattacharya, Sudha |
author_sort | Singh, Nishant |
collection | PubMed |
description | Homologous recombination (HR) has not been demonstrated in the parasitic protists Entamoeba histolytica or Entamoeba invadens, as no convenient method is available to measure it. However, HR must exist to ensure genome integrity, and possible genetic exchange, especially during stage conversion from trophozoite to cyst. Here we show the up regulation of mitotic and meiotic HR genes in Entamoeba during serum starvation, and encystation. To directly demonstrate HR we use a simple PCR-based method involving inverted repeats, which gives a reliable read out, as the recombination junctions can be determined by sequencing the amplicons. Using this read out, we demonstrate enhanced HR under growth stress in E. histolytica, and during encystation in E. invadens. We also demonstrate recombination between chromosomal inverted repeats. This is the first experimental demonstration of HR in Entamoeba and will help future investigations into this process, and to explore the possibility of meiosis in Entamoeba. |
format | Online Article Text |
id | pubmed-3787063 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-37870632013-10-04 Homologous Recombination Occurs in Entamoeba and Is Enhanced during Growth Stress and Stage Conversion Singh, Nishant Bhattacharya, Alok Bhattacharya, Sudha PLoS One Research Article Homologous recombination (HR) has not been demonstrated in the parasitic protists Entamoeba histolytica or Entamoeba invadens, as no convenient method is available to measure it. However, HR must exist to ensure genome integrity, and possible genetic exchange, especially during stage conversion from trophozoite to cyst. Here we show the up regulation of mitotic and meiotic HR genes in Entamoeba during serum starvation, and encystation. To directly demonstrate HR we use a simple PCR-based method involving inverted repeats, which gives a reliable read out, as the recombination junctions can be determined by sequencing the amplicons. Using this read out, we demonstrate enhanced HR under growth stress in E. histolytica, and during encystation in E. invadens. We also demonstrate recombination between chromosomal inverted repeats. This is the first experimental demonstration of HR in Entamoeba and will help future investigations into this process, and to explore the possibility of meiosis in Entamoeba. Public Library of Science 2013-09-30 /pmc/articles/PMC3787063/ /pubmed/24098652 http://dx.doi.org/10.1371/journal.pone.0074465 Text en © 2013 Singh et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Singh, Nishant Bhattacharya, Alok Bhattacharya, Sudha Homologous Recombination Occurs in Entamoeba and Is Enhanced during Growth Stress and Stage Conversion |
title | Homologous Recombination Occurs in Entamoeba and Is Enhanced during Growth Stress and Stage Conversion |
title_full | Homologous Recombination Occurs in Entamoeba and Is Enhanced during Growth Stress and Stage Conversion |
title_fullStr | Homologous Recombination Occurs in Entamoeba and Is Enhanced during Growth Stress and Stage Conversion |
title_full_unstemmed | Homologous Recombination Occurs in Entamoeba and Is Enhanced during Growth Stress and Stage Conversion |
title_short | Homologous Recombination Occurs in Entamoeba and Is Enhanced during Growth Stress and Stage Conversion |
title_sort | homologous recombination occurs in entamoeba and is enhanced during growth stress and stage conversion |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3787063/ https://www.ncbi.nlm.nih.gov/pubmed/24098652 http://dx.doi.org/10.1371/journal.pone.0074465 |
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